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Use of bovine-RBC-substituted SCID mice for the studies of Babesia parasites

Research Project

Project/Area Number 07456146
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionRakuro-gakuen University

Principal Investigator

TSUJI Masayoshi  Rakuno-gakuen Univ., Scool of Vet.Med., Associate Prof., 獣医学部, 助教授 (10150088)

Co-Investigator(Kenkyū-buntansha) MIYAZAWA Masaaki  Kinki Univ., Scool of Medicine, Professor, 医学部, 教授 (60167757)
ISHIHARA Chiaki  Rakuno-gakuen Univ., Scool of Vet.Med., Professor, 獣医学部, 教授 (90082172)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsSCID mice / Animal model / Babesia ovata / Babesia bovis / Cerebral babesiosis / Antigenic variation / Babesia
Research Abstract

Various experimental systems were developed for the studies of Babesia parasites using bovine-red blood cell-substituted (Bo-RBC-SCID mice), and following results were obtained
(1) Isolation of a new Babesia parasite : Using Bo-RBC-SCID mice, a new bovine Babesia parasite (Oshima strain) were isolated from grazing cattle in Hokkaido, Japan. The isolate differed from Miyake strain, which is the type strain of Babesia ovata, in terms of morphology, antigenic structure and genomic DNA,but shared the same vector tick, Haemaphysalis longicornis. Thus, Babesia ovata oshimensis n. var. has been proposed. Bo-RBC-SCID mice infected with Babesia parasites showed various clinical symptoms, including hemoglobinuria, jaundice and nerve symptoms, and therefore may serve as a useful model system for studying the mechanisms of disease caused by Babesia parasites.
(2) Persistent infection and antigenic variation of Babesia : Epidemiological survey with parasite isolation using Bo-RBC-SCID mice revealed t … More hat Babesia parasites persistently infect cattle in Okushiri island, Hokkaido. Significant variations in antigenic profiles and genomic DNA were observed among the samples obtained at different time points from a single infected animal, implicating possible antigenic variations. Monoclonal antibodies against B.ovata Oshima strain has been made, and a clone, 1A2, which produces antibody inhibitory to the parasite growth in Bo-RBC-SCID mice was selected. The 1A2 antibody was found to recognize a 56-kDa merozoite surface protein (P56) which may potentially be involved in antigenic variation. The cDNA clone encoding P56 was obtained, sequenced, and expressed in E.coli.
(3) A mouse model for cerebral babesiosis : Bo-RBC-SCID mice infected with Babesia bovis were found to display central nerve symptoms. Light microscopy revealed sequestration of parasitized RBC in the microvessels in the brain of SCID mice. Electron microscopy showed the presence of "knob" like conical projections on the surface of infected RBCs, with which the parasitized RBCs appeared to adhere to the capillary endothelium in SCID mice brain. Those findings are strikingly similar to those found in bovine cerebral babesiosis and also in human cerebral malaria, and therefore B.bovis-infection in Bo-RBC-SCID mice can be used as a model for those disease. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (55 results)

All Other

All Publications (55 results)

  • [Publications] Tsuji M., Terada Y., Arai S., Okada H., and Ishihara C: " Use of the Bo-RBC-SCID mouse model for isolation of a Babesia parasite from grazing calves in Japan" Exp. Parasitol. 81. 512-518 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakamura Y., Tsuji M., Arai S., and Ishihara C.: "Amethod for rapid and complete substitution of the circulating erythrocytes in SCID mice with bovine erythrocytes and use of the substituted mice for bovine hemoprotozoa infection." J.Immunol. Methods. 188. 247-254 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohta M., Tsuji M., Tsuji N., and Fujisaki K: "Morphological, serological and antigenic characteristics, and protein profile of newly isolated Japanese bovine Babesia parasite with particular reference to those of B.ovata." J. Vet. Med. Sci.,. 57. 671-675 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji M., Ishihara C., Arai S., Hiratai R., and Azuma l.: "Establishment of a SCID mouse model having circulating human red blood cells and a possible growth of Plasmodium falciparum in the mouse" Vaccine. 13. 1389-1392 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada Y., Tsuji M., Hagiwara K., Takahashi K., and Ishihara C.,: "Clearance of Theileria sergentiinfected bovine red blood cells in SCID mice." Vet. Parasitol. 60. 221-228 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hagiwara K., Tsuji M., Ishihara C., Tajima M., Kurosawa T., and Takahashi K.,: "Serum from Theileria sergenti infected cattle accelerate the clearance of bovine erythrocytes in SCID mice. Parasitol. Res." 81:. 470-474 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishihara C., Shimakawa S., Tsuji M., Arikawa J., and Tokura S: "Asulfated chitin, SCM-chitin lll, inhibits the clearance of human erythrocytes from the blood circulation in the erythrocyte- transfused SCID mice." Immunopharmacology. 29. 65-71 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi T., Tsuji M., Kikuchi N., Ishihara C., Osanai T. Kasai N., Yanagawa R., and Hiramune T.: "Assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16S rRNA genes of Corynebacteria." J.Vet. Med.Sci.57(3). 515-517 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wei Q., Tsuji M., Takahashi T., Ishihara C., and Itoh T.: "Taxonomic status of CAR bacillus based on the small subunit ribosomal RNA sequences." Chin Med. Sci. J.,. 10. 195-198 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohta M., Kawazu S., Terada Y., Kamio T., Tsuji, M.and Fujisaki K.: "Experimental transmission of Babesia ovata oshimensis n. var. of cattle in Japan by Haemaphysalis longicornis." J.Vet. Med.Sci.,. 58:. 1153-1155 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji M., Fujioka S., Arai S., Tanayama H., Ishihara C., and Aikawa M.: "Amouse model for cerebral babesiosis" Parasitol. Today. 12. 203-205 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi T., Kaneko M., Mori Y., Tsuji M., Kikuchi N., and Hiramune T: "Phylogenetic analyses of Staphylococcus based on the 16S rRNA sequence and assignment of clinical isolates from animals" J.Vet. Med.Sci.,. 59. 775-783 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hagiwara K., Takahashi M., Ichikawa T., Tsuji M., Ikuta K., and Ishihara C: "Inhibitory effect of heparin on red blood cell invasion by Theileria sergenti merozoites." Int.J.Parasitol. 27. 535-539 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hagiwara K., Takahashi K., Taniyama H., Kawamoto S., Kurosawa T., Ikuta K., and Ishihara C.: "Detection of Theileria sergenti schizonts in bovine lymph node." Int. J. Parasitol.27(11). 1375-1378 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishihara C., Miyamoto A., Kim S., Arai S., Taniyama H., Maejima K., and Tsuji M.: "Candida guilliermondii infection in SCID mice in association with the acceleration for the elimination of transfused human red cells." Exp. Anim.,. 47(1). 69-73 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada Y., Tsuji M., Nakamura Y., Hagiwara K., Takahashi K., and Ishihara C.: "Amethod for analysis of cell to cell transmission of Theileria sergenti piroplasms." J.Vet Med.Sci.,. 60(1). 115-117 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishihara C., Hiratai R., Tsuji M., Yagi K., Nose M.and Azuma I.: "Mannan decelerate the clearance of human red blood cells in SCID mouse." Immunopharmacology. 38. 223-228 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kim S-J., Tsuji M., Kubota S., Wei Q., Lee J-M, Ishihara C., and Onuma M.: "Sequence analysis of the major piroplasm surface protein gene of benign bovine Theileria parasites in East Asia." Int. J. Parasitol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Araia S., Tsuji M., Kim S-J., Nakadea T., Kanno Y., and Ishihara C.: "Babesia canis infection in canine-red blood cell-substituted SCID mice" Int.J.Parasitol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 辻 正義: "リボゾームRNAの塩基配列に基づくティザ-菌(Clostridium piliforme)の系統分類" アニッテクス. 7(2). 19-25 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石原 智明、辻 正義: "SCID マウス" 小児外科. 29(2). 260-6 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 辻 正義、新井 智、金 三柱、萩原 克郎、石原 智明: "ヒトとマラリアのモデル-赤血球置き換えSCIDマウスを用いた住血原虫症の研究-" 日本疾患モデル学会. 13. 29-35 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石原 智明: "scid マウス、笠井 憲雪・板垣真一編:疾患モデル動物、前島一淑・長澤弘監修:バイオメデイカルリサーチマニュアル、第4巻" 養賢堂, 168-76 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 石原 智明: "感染症解析のための動物実験モデルの作出とその応用、坪倉・平棟・金子編:微生物の世界-微生物とヒト・動物とのかかわり-" 養賢堂、東京, 225-41 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 辻 正義: "ヒトマラリアのモデル-赤血球置き換えSCIDマウスを用いた住血原虫症の研究-、安倍 千之編;SCID・疾患モデル研究" 日本医学館、東京, 107-114 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji M., Terada Y., Arai S., Okada H., and Ishihara C.: "Use of the Bo-RBC-SCID mouse model for isolation of a Babesia parasite from grazing calves in Japan" Exp. Parasitol.81. 512-518 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakamura Y., Tsuji M., Arai S., and Ishihara C.: "A method for rapid and complete substitution of the circulating erythrocytes in SCID mice with bovine erythrocytes and use of the substituted mice for bovine hemoprotozoa infection" J.Immunol. Methods. 188. 247-254 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohata M., Tsuji M., Tsuji N., and Fujisaki K.: "Morphological, serological and antigenic characteristics, and protein profile of newly isolated Japanese bovine Babesia parasite with particular reference to those of B.ovata" J.Vet. Med. Sci.57. 671-675 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji M., Ishihara C., Arai S., Hiratai R., and Azuma I.: "Establishment of a SCID mouse model having circulating human red blood cells and a possible growth of Plasmodium falciparum in the mouse" Vaccine. 13. 1389-1392 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada Y., Tsuji M., Hagiwara K., Takahashi K., and Ishihara C.: "Clearance of Theileria sergenti-infected bovine red blood cells in SCID mice" Vet. Parasitol.60. 221-228 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hagiwara K., Tsuji M., Ishihara C., Tajima M., Kurosawa T., and Takahashi K.: "Serum from Theileria sergenti infected cattle accelerate the clearance of bovine erythrocytes in SCID mice" Parasitol. Res.81. 470-474 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishihara C., Shimakawa S., Tusuji M., Arikawa J., and Tokura S.: "A sulfated chitin, SCM-chitin III, inhibits the clearance of human erythrocytes from the blood circulation in the erythrocyte-transfused SCID mice" Immunopharmacology. 29. 65-71 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi T., Tsuji M., Kikuchi N., Ishihara C., Osanai T., Kasai N., Yanagawa R., and Hiramune T.: "Assignment of the bacterial agent of urinary calculus in young rats by the comparative sequence analysis of the 16S rRNA genes of Corynebacteria" J.Vet. Med. Sci.57(3). 515-517 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wei Q., Tsuji M., Takahashi T., Ishihara C., and Itoh T: "Taxonomic status of CAR bacillus based on the small subunit ribosomal RNA sequences" Chin. Med. Sci. J.10. 195-198 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohta M., Kawazu S., Terada Y., Kamio T., Tsuji M., and Fujisaki K.: "Experimental transmission of Babesia ovata oshimensis n. var. of cattle in Japan by Haemaphysalis longicornis." J.Vet. Med. Sci.58. 1153-1155 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuji M., Fujioka S., Arai S., Tanayama H., Ishihara C., and Aikawa M.: "A mouse model for cerebral babesiosis" Parasitol. Today. 12. 203-205 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takahashi T., Kaneko M., Mori Y., Tsuji M., Kikuchi N., and Hiramune T.: "Phylogenetic analyzes of Staphylococcus based on the 16S rRNA sequence and assignment of clinical isolates from animals" J.Vet. Med. Sci.59. 775-783 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hagiwara K., Takahashi M., Ichikawa T., Tsuji M., Ikuta K., and Ishihara C.: "Inhibitory effect of heparin on red blood cell invasion by Theileria sergenti merozoites." Int. J.Parasitol.27. 535-539 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hagiwara K., Takahashi K., Taniyama H., Kawamoto S., Kurosawa T., Ikuta K., and Ishihara C.: "Detection of Theileria sergenti schizonts in bovine lymph node" Int. J.Parasitol.27(11). 1375-1378 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishihara C., Miyamoto A., Kim S., Arai S., Taniyama H., Maejima K., and Tsuji M.: "Candida guilliermondii infection in SCID mice in association with the acceleration for the elimination of transfused human red cells." Exp. Anim.47(1). 69-73 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada Y., Tsuji M., Nakamura Y., Hagiwara K., Takahashi K., and Ishihara C.: "A method for analysis of cell to cell transmission of Theileria sergenti piroplasms." J.Vet. Med. Sci.60(1). 115-117 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishiara C., Hiratai R., Tsuji M., Yagi K., Nose M., and Azuma I.: "Mannan decelerate the clearance of human red blood cells in SCID mouse." Immunopharmacology. 38. 223-228 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Km S-J., Tsuji M., Kubota S., Wei Q., Lee J-M., Ishihara C., and Onuma M.: "Sequence analysis of major piroplasm surface protein gene of benign bovine Theileria parasites in East Asia." Int. J.Parasitol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Araia S., Tsuji M., Kim S-J., Nakadea T., Kanno Y., and Ishihara C.: "Babesia canis infection in canine-red blood cell-substituted SCID mice" Int. J.Parasitol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ishihara C.,Hiratai R.,et al.: "Mannan decelerate the clearance of human red blood cells in SCID mouse." Immunopharmacology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Terada Y.,Tsuji M.et al.: "A method for analysis of cell to cell transmission of Theileria sergenti piroplasms." J.Vet.Med.Sci.60(1). 115-117 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ishihara C.,Miyamoto A.et al.: "Candida guilliermondii infection in SCID mice in association with the acceleration of the elimination of transfused human red blood cells." Exp.Anim.47(1). 69-73 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hagiwara K.,Takahashi M.et al.: "Inhibitory effect of heparin on red blood cell invasion by Theileria sergenti merozoites." Int.J.Parasitol.27(5). 535-539 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masayoshi Tsuji et al.: "A mouse model for cerebral baesiosis" Parasitol.Today. 12. 203-205 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masata Ohta et al.: "Experimental transmission of Babesia ovata oshimensis n.var.of Cattle in Japan by Haemaphysalis longicornis" J.Vet.Med.Sci.58. 1153-1155 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masayoshi Tsuji et al.: "Use of the Bo-RBC-SCID mouse model for isolation of a Babesia parasite from grazing calves in Japan." Experimental Parasitology. 81. 512-518 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masato Ohta et al.: "Morphological, serological and antigenic characteristics, and protein of newly isolated Japanese bovine Babesia parasite with particular reference to those of B. ovata." Journal of Veterinary Medical Science. 57. 671-675 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yurie Nakamura et al.: "A method for rapid and complete substitution of the circulating erythrocytes in SCID mice with bovine erythrocytes and use of the substituted mice for bovine hemoprotozoa infections." Journal of immunological methods. 188. 247-254 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yutaka Terada et al.: "Clearance of Theileria sergenti-infected bovine red blood cells in severe combined immune deficiency mice." Veterinary Parasitology. 60. 221-228 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayoshi Tsuji et al.: "A mouse model for cerebral babesiosis" Parasitology Today. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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