| Project/Area Number |
07456151
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
作物学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
KUBOTA Fumitake Kyushu Univ., Agriculture Dept., Professor, 農学部, 教授 (50136602)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Chlorophyll quenching / CO_2 assimilation / Gas exchange rate / Intermittent Illumination / Photosystem / RuBP Oxygenase / RuBP Carboxylase / Stomata / ガス交換 / クロロフィル / 蛍光消光 / 個葉 / 光合成 / 明反応系 / 暗反応系 / 蛍光発光 / 炭酸ガス交換速度 / マングビーン |
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| Research Abstract |
This study was conducted to understand the CO_2 assimilation-governing mechanism by relating the leaf CER (CO_2 exchange rate) with the chemical energy production-consumption balance between photosystem and CO_2 assimilation. (1) CER of mungbean leaves were determined as a time course under the intermittent illumination with 20 second-intervals of light on/off in the different combinations of CO_2 and O_2 concentrations and light intensities. Based on the calculation of the energy produced at the light-on (20s) moments and the weight of CO_2 assimilated at the light-off (20s) moments, the effect of the intermittent illumination on the Rubisco (RuBP Carboxylase/Oxygenase) activity was estimated. CER or light utilization efficiency was increased at the intermittent illumination in high light intensities, because CO_2 was possible to be assimilated at the light-off moments by using the accumulated energy at the light-on moments. Light activation level of Rubisco varied with CO_2 and O_2 concentrations. The relative activity between carboxylase and oxygenase differed depending on the environmental conditions, affecting the CER and light utilization efficiency at the intermittent illumination. (2) Then, to get more clear explain for the action balance between photosystem and CO_2 assimilation system, the relationships between CER and chlorophyll fluorescence quenching were studied. The improved measurement tools were skillfully assembled to make a simultaneous determination of both factors. It was evident in mungbean leaves that the energy consumption by photorespiration significantly affected, in relation to environmental factors, both CER and the potimal energy utilization efficiency in photosynthesis.
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