Regulatory Mechanism for Oxalate Production by the Enzymes and Its Related System in Wood-rotting Fungi.
| Project/Area Number |
07456155
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIMADA Mikio Kyoto University, Wood Research Institute, Professor, 木質科学研究所, 教授 (50027166)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Takefumi Kyoto University, Wood Research Institute, Research Instructor, 木質科学研究所, 助手 (60212148)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Oxalic acid / Glyoxylic acid / Flavohemoprotein / Glyoxylate dehydrogenase / Brown-rot fungi / White-rot fungi / Basidiomycetes / Tyomyces palustris / Oxalic acid / Glyoxylic acid / Biosynthesis / Glyoxylate oxidase / White-rot fungi / Brown-rot fungi / Basidiomycetes / Tyromyces palustris / Wood-rotting fungi / グリオキシレート / グリオキシレート酸化酵素 / カワラタケ / 木材腐朽 / 担子菌 / 褐色腐朽 |
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| Research Abstract |
A role of oxalate producing enzyme systems in wood rotting fungi has not been elucidated. Most of brown rot fungi which attack wooden parts of houses have a strong activity to produce oxalic acid and they are tolerant to copper wood preservaties, including CCA (Copper, chrome and arsenate), because they detoxicate the copper with oxalate produced to form insoluble copper oxalate.
As a result, following findings have been obtained.
1.Oxalate which was known to inhibit the one elctron oxidation by lignin peroxidase was found also to inhibit the cleavage nonphenolic beta-O-4LCC model compound between lignin model moiety and glucose.
2.Three enzymes, including oxalate producing enzyme (glyoxylate oxidase), oxalate decarboxylase, and formate dehydrogenase were isolated from a white-rot fungus Coriolus versicolor. Formate dehydrogenase was first cell-free extracted from wood rotting fungi by this investigation.
3.We succeeded in isolating and purifying a new type of cytochrome c dependent glyoxylate dehydrogenase from copper tolerant brown rot fungus Tyromyces palustris. Spectral analysis of the enzyme showed that it belongs to a family of flavohemoproteins. FMN was found to be a prosthetic group. Thus, oxalate production was found to be catalyzed by the electron transport system with cytochrome c and may be controlled by inhibition of the electron transportation system.
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Report
(4 results)
Research Products
(13 results)
