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DIRECT IMAGING OF E.coil POLYMERASE AND TRANSCRIPTION COMPLEX WITH PROMOTER REGION OF MUTANT DNA (UV5).

Research Project

Project/Area Number 07457005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagoya University

Principal Investigator

USUKURA Jiro  Associate professor of Department of anatomy Nagoya University School of Medicine, 医学部, 助教授 (30143415)

Co-Investigator(Kenkyū-buntansha) OBATA Shuichi  Assistant of Department of anatomy Nagoya University School of Medicine, 医学部, 助手 (10204273)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
Keywordslow angle shadowing / DNA / transcription / RNA polymerase / CREB / CRP / E.coil / Electron microscopy / 転写
Research Abstract

Improved low angle rotary shadowing was available for structural analysis of RNA polymerase and its binding to DNA (transcription complex). The shadowed image of the polymerase was basically composed of four subunits in two different sizes and one rod like structure. A dimeric structure of two big subunits was stacked on another small dimeric structure, forming large channel between them, 3nm in width. Rod like structure was found in this channel. Big and small subunits seem to correspond to b, b^ユ and 2 a subunits respectively, judging from differences in their molecular weight that have been revealed biochemically so far. On binding with the promoter region of DNA,DNA strand was found in this large channel. Comparative observation of core enzyme without s factor suggested that s factor should be rod like structure placed along this channel together with DNA.Another narrow space was recognizable between b and b^ユ subunits. Taking the affinity of b subunit to mRNA into account, this slit may be a channel for newly synthesizing mRNA.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Arai, M., Nishizawa, Y. et al.: "Confocal imaging of megamitochondria formation induced by ethanol and hydrazine in culture cells (RL-34)." Bioimaes. Vol. 3. 25-30 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Adachi, K., Matsuhashi, T. et al.: "Studies on urea synthesis in the liver of rats treated chronically with ethanol using perfused livers, isolated..." Biochem. Pathol.vol. 23. 667-676 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Adachi, K., Matsuhashi, T. et al.: "Suppression of the hydrazine-induced formation of megamitochondria in the rat liver by coenzyme." Toxicol. Lett. vol. 86. 47-54 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taya, S., Taniguchi, Y., et al.: "Development of γ type energy filtering TEM." J. Electrom. Microsc.Vol. 45. 307-313 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazaki, A., Bondarenko, V. A., et al.: "Possible stimulation of retinal rod recovery to dark state by cGMP release from a cGMP phosphodiesterase noncatalytic site." J. Biol. Chem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirako, Y., Usukura, J., et al.: "Demonstration of the molecular shape of BP180, a 180-kDa bullous pemphigoid antigen and its potential for trimer formatior." J. Biol. Chem.vol. 271. 13739-13745 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arai, M., Nishizawa, Y., et al.: "Confocal imaging of megamitochondria formation induced by ethanol and hydrazine in culture cells (RL-34)." Bioimages. vol.3. 25-30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Adachi, K., Matsuhashi, T., et al.: "Studies on urea synthesis in the liver of rats treated chronically with ethanol using perfused livers, isolated..." Biochem.Pathol.vol.23. 667-676 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taya, S., Taniguchi, Y., et al.: "Development of g-type energy filtering TEM." J.Electrom.Microsc.vol.45. 307-313 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Adachi, K., Matsuhashi, T., et al.: "Suppression of the hydrazine-induced formation of megamitochondria in the rat liver by coenzyme." Toxicol.Lett.vol.86. 47-54 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazaki, A., Bondarenko, V.A., et al.: "Possible stimulation of retinal rod recovery to dark state by cGMP release from a cGMP phosphodiesterase noncatalytic site." J.Biol.Chem.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroko, Y., Usukura, J., et al.: "Demonstration of the molecular shape of BP180, a 180-kDa bullous pemphigoid antigen and its potential for trimer formatior." J.Biol.Chem.vol.271. 13739-13745 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arai,M.,Nishizawa,Y.et al.: "Confocal imaging of megamitochondria formation induced by ethanol and hydrazine in culture cells(RL-34)." Bioimaes. Vol.3. 24-30 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Adachi,K.,Matsuhashi,T.et al.: "Studies on urea synthesis in the liver of rats treated chronically with ethanol using perfused livers,isolated..." Biochem.Pathol.vol.23. 667-676 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Adachi,K.,Matsuhashi,T.et al.: "Suppression of the hydrazine-induced formation of megamitochondria in the rat liver by coenzyme." Toxicol.Lett. vol.86. 47-54 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taya,S.,Taniguchi,Y.,et al.: "Development of γ type energy filtering TEM." J.Electrom.Microsc.vol.45. 307-313 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamazaki,A.,Bondarenko,V.A.,et al.: "Possible stimulation of retinal rod recovery to dark state by cGMP release from a cGMP phosphodiesterase noncatalytic site." J.Biol.Chem.(in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirako,Y.,Usukura,J.,et al.: "Demonstration of the molecular shape of BP180,a 180-kDa bullous pemphigoid antigen and its potential for trimer formatic" J.Biol.Chem.vol.271. 13739-13745 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Usukura,J.: "Morphogenesis of photoreceptor outer segments in retinal development." Prog. Retinal Eye Res.,. 15. 113-125 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hirako,Y.: "Demonstration of the molecular shape of BP 180 and its potention for trimer formation." J. Biol. Chem.(in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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