Mechanisms of resistance against rabies virus by anti-nucleoprotein (N) antibodies and the analysis of localization of the N in the virion
| Project/Area Number |
07457079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Oita Medical University |
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Principal Investigator |
MIFUNE Kumato Oita Medical University, Department of Microbiology, Professor, 医学部, 教授 (70039915)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIZONO Akira Oita Medical University, Department of Microbiology, Research Assoc., 医学部, 助手 (70218155)
SHICHIJO Akehisa Oita Medical University, Department of Microbiology, Associate Prof., 医学部, 助教授 (90039917)
MANNEN Kazuaki Oita Medical University, Animal Laboratory Center, Assistant Professor, 医学部, 助教授 (20145361)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Rabies virus / Internal proteins of rabies virion / Anti-nucleoprotein antibodies / Resistance against rabies virus / Immunoelectronmicroscopic study / ウイルス内部構成蛋白 / 感染防御機構 / 核蛋白粒子内局在 |
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| Research Abstract |
By using the techniques of pre-embedding colloidal gold method in immunoelectronmicroscopic study, positive reactions were observed on the surface of rabies virions being budded from virus-infected cells when stained with anti-nucleoprotein (N) polyclonal and monoclonal antibodies. This positive reaction disappeared by prior-absorption of the antibodies with excess amount of N.Anti-glycoprotein polyclonal antibodies also exhibited positive reactions but control antibodies did not.
The epitopes which can be recognized by anti-N antibodies were not expressed on the surface of virus-infected cells as determined by FACScan and complement-mediated cell lysis experiment with anti-N antibodies, although the epitopes recognized by cytotoxic T lymphocytes were shown to be expressed in our previous studies.
Our preliminary experiments demonstrated that hemagglutination of human erythrocytes by rabies virus is not inhibited in the presence of anti-N antibodies, however, subsequent hemolysis after exposure of the erythrocytes to acid environment is significantly inhibited by anti-N antibodies.
Possible significance of the positive reactions with anti-N antibodies observed on the surface of rabies virions was discussed.
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Report
(3 results)
Research Products
(4 results)
