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Phenotypic and functional analysis of Dendritic cells in Liver

Research Project

Project/Area Number 07457083
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionKyoto University

Principal Investigator

INABA Kayo  Graduate School of Science, Department of Zoology, Kyoto University Associate Professor, 大学院・理学研究科, 助教授 (00115792)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥5,200,000 (Direct Cost: ¥5,200,000)
Keywordsdendritic cells / liver / cytokines / maturation / phenotype / function / MHCクラスII
Research Abstract

Dendritic cells (DCs) are a system of potent antigen-presenting cells that are distributed in lymphoid and nonlymphoid tissues where they are usually distinguished by their morphology and class II antigen expression. DCs in peripheral nonlymphoid tissues are thought to be a relatively immature cell type that capture antigens and migrate to T areas of the draining lymphoid organs. However, heterogeneity of DCs in nonlymphoid tissues is not well known. Therefore, the aim of this project is further characterization of DCs in terms of phenotype, function and distribution in the liver.
A procedure was developed to maximize the yield of viable, nonparenchymal hepatic leukocytes by collagenase digestion of perfused liver fragments followed by density centrifugation with Nyco Prep^<TM>. FACS analyzes on isolates showed that N418 (CD11c)^+ cells are categorized into three different subpopulations besed on the cell size and the expression of class II,CD86 and DEC-205. All of them were CD3^-, CD4^-, and CD8^-, but strongly expressed CD45 and moderate levels of CD11b and CD44. The most immature DCs were small in size and negative or very little for class II,but expressed low level of B220. The intensity of class II was shown to correlate to the cell size. The large Class II^<high> cells with dendritic shape primarily localized in the triads but not in sinus, and CD11c^+ cells in sinus were negative for class II and relatively small round cells in immunohistochemical observation. When even small CD11c^+ cells, however, were cultured in the presence of GM-CSF for a few days, they became larger and increased class II,CD86 and DEC-205 expression, and potent allostimulatory activity was detected. These observations indicate that developmentally different populations of DCs are present in the distinguished part of the liver.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] Umezu, H., Naito, M., Inaba, K. et al.: "Ultrastructural and immunophenotypic differentiation of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor(GM-CSF)." J. Submicrose, Cytol. Pathol.27. 227-234 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K., Swiggard, W., Inaba, M., et al.: "Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes." Cell. Immunology. 163. 145-156 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Witmer-Pack, M. D., Swiggard, W. J., Inaba, K., et al/: "Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II. Expression in situ in lymphoid and nonlymphoid tissues." Cell. Immunology. 163. 157-162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Haruna, H., Inaba, M., Inaba, K., et al.: "Abnormalities of B cells and dendritic cells in SAMP1 mice." Eur. J. Immunol.25. 1319-1325 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K. and Inaba, M.: "Dendritic cells in airway(Japanese)" Respiration Research. 14. 826-831 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hagi, K., Inaba, K., Sakuta, H. and Muramtsu, S: "Enhancement of murine bone marrow macrophage differentiation by β-endorphin." Blood. 86. 1316-1325 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K., Inaba, M., Witemr-Pack, M., et al.: "Expression of B7 costimulator molecules on mouse dendritic cells." Adv. Exp. Med. Biol.378. 65-70 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi, K., Naito, M., Inaba, K. and Akagawa, K.: "Development and differentiation of dendritic cells-Two functionally related but ultrastructurally distinct dendritic cell subpopulationns." Dendritic cells. 5. 1-5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Granelli-Piperno, A., Pope, M., Inaba, K. and Steinman, R, M/: "Coexpression of Rel and SP-1 transcription factors in HIV induced, dendritic cell-T cell syncytia" Proc. Natl. Acad. Sci. USA. 92. 10944-10948 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Young, J. W. and Inaba, K.: "Dendritic cells as adjuvants for class I restricted anti-tumor immunity." J. Exp. Med.183. 7-11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fazle Akbar, S. M., Inaba, K. and Onji, M.: "Upregulation of MHC class II antigen on dendritic cells from hepatitis B virus transgenic mice by γ-interferon : abrogation of immune response defect to a T-cell-dependent antigens." Immunology. 87. 519-527 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ortner, U., Inaba, K., Koch F., et al.: "An improved method to obtain murine migratory cutaneous dendritic cells." J. Immunol. Methods. 193. 71-79 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K.: "Endocytic activity of dendritic cells and role of C-type lectin for antigen presentation. (Japanese)" Clinical Immunol.29. 141-147 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaguchi, Y., Tumura, H., Miwa, M. and Inaba, K.: "Contrasting effect of TGF β1 and TNFα on the development of dendritic cells from progenitors" Stem cells. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Doi, H., Inaba, M., Yamamoto, Y., et al.: "Pluripotent hemopoietic stem cells are c-kit^<<xlow>." Proc. Natl. Acad. Sci. USA. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K.: "Dendritic cells as antigen pressnting cells in vivo." Immunol. Cell Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Umezu, H., Naito, M., Inaba, K.and Takahashi, K.: "Ultrastructural and immunophenotypic differentiation of dendritic cells form mouse bone marrow cultures supplemented with granulocyte/macrophage colonystimulating factor (GM-CSF)." J.Submicrosc, Cytol.Pathol. 27. 227-234 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K., Swiggard, W., Inaba, M., Meltzer, J., Mirza, A., Sasagawa, T., Nussenzweig, M.C.and Steinman, R.M.: "Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I.Expression on dendritic cells and other subsets of mouse leukocytes" Cell.Immunol.163(1). 145-156 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Witmer-Pack, M.D., Swiggard, W.J., Inaba, K., Mirza, A.and Steinman, R.M.: "Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. II.Expression in situ in lymphoid and nonlymphoid tissues" Cell.Immunol.163(1). 157-162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Haruna, H., Inaba, M., Inaba, K., Taketani, S., Sugiura, K., Fukuba, Y., Doi, H., Tokunaga, R., and Ikehara, S.: "Abnormalities of B cells dendritic cells in SAMPl mice" Eur.J.Immunol.25(5). 1319-1325 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K.and Inaba, M.: "Dendritic cells in airway (Japanese)" Respiration Research. 14(8). 826-831 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hagi, K., Inaba, K., Sakuta, H.and Muramtsu, S.: "Enhancement of murine bone marrow macrophage differenttiation by beta-endorphin." Blood. 86(4). 1316-1321 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, k., Inaba, .M., Witemr-Pack, M., Hathcock, K., Hodes, R., and Steinman, R.M.: "Expression of B7 costimulator molecules on mouse dendritic cells." Adv.Exp.Med.Biol.378. 65-70 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi, K., Naito, M., Inaba, K.and Akagawa, K.: "Development and differentiation of dendritic cells-Two functionally related but ultrastructurally distinct cell subpopulations." Dendritic cells. 5. 1-5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Granelli-Piperno, A., Pope, M., Inaba, K.and Steinman, R.M: "Coexperssion of Rel and SP-1 transcription factors in HIV induced, dendritic cell-T cell syncytia" Proc.Natl.Acad.Sci.USA.92(24). 10944-10948 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Young, J.W.and Inaba, K.: "Dendritic cells as adjuvants for class I restricted anti-tumor immunity." J.Exp.Med.183(1). 7-11 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fazle Akbar, S.M., Inaba, K.and Onji, M.: "Upregulation of MHC class II antigen on dendritic cells from hepatitis B virus transgenic mice by gamma-interferon : abrogation of immune response defect to a T-cell-dependent antigens." Immunology. 87. 519-527 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ortner, U., Inaba, K., Koch F., Heine, M., Miwa, M., Schuler, G.and Romani, N.: "An improved method to obtain murine migratory cutaneous dendritic cells." J.Immunol.Methods. 193. 71-79 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba K.: "Endocytic activity of dendritic cells and role of C-type lectin for antigen presentation. (Japanese)" Clinical Immunology. 29(1). 141-147 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaguchi, Y., Tumura, H., Miwa, M.and Inaba, K.: "Contrasting effect of TGFbeta1 and TNFalpha on the development of dendritic cells from progenitors in mouse bone marrow" Stem Cells. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Doi, H., Inaba, M., Yamamto, Y., Taketani, S., Mori, S.-I., Sugira, A., Ogata, H., Toki, J., Hisha H., Inaba, K., Sogo, S., Adachi, M., Matsuda, T., Good, R.A.and Ikehara, S.: "Pluripoent hemopoietic stem cells are c-kit<low." Proc.Natl.Acad.Sci.USA. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K.: "Dendritic cells as antigen presenting cells in vivo." Immunol.Cell.Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Young,J.W.: "Dendritic cells as adjuvants for class I restricted anti-tumor immunity." J.Exp.Med. 183. 7-11 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Frazle Akbar: "Upregulation of MHC class II antigen on dendritic cells from hepatitis B virus transgenic mice by γ-interferon : abrogation of immune response defect to a T-cell-dependent antigens" Immunology. 87. 519-527 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ortner,U.: "An improved method to obtain murine migratory cutaneous dendritic cells." J.Immunol.Methods. 193. 71-79 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakuta,H.: "Calcitonin gene-related peptide enhances apoptosis of thymocytes." J.Neurommunol.67. 103-109 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inaba,K.: "Dendritic cells as antigen presenting cells in vivo." Immunol.Cell Biol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamaguchi,Y.: "Contrasting effect of TGFβ1 and TNFα on the development of dendritic cells from progenitors in mouse bone marrow." Stem Cells. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] K. Inaba: "Tissue distribution of DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes" Cell. Immunol.163. 145-156 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Haruna: "Abnormalities of B cells and dendritic cells in SAMP1 mice" Eur. J. Immunol.25. 1319-1325 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Inaba: "Expression of B7 costimulatory molecules on mouse dendritic cells" Adv. Exp. Med. Biol.378. 65-70 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Takahashi: "Development and differentiation of dendritic cells-Two functionally related but ultrastructually didtincttually distinct dendritic cell subpopulation" Dendritic Cells. 5. 1-5 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Granelli-Piperno, A: "Coexpression of Rel and SP-1 transcription factors in HIV induced, dendritic cell-T cell syncytia" Proc. Natl. Acad. Sci. USA. 92. 10944-10948 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Frazle Akbar: "Upregulation of MHC class II antigen on dendritic cells from hepatitis B virus transgenic mice by γ-interferon: abrogation of immune response defect to a T-cell-dependent antigens" Immunology. (in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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