| Project/Area Number |
07457144
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIMURA Sanae Tohoku Univ.Hospital, Lecturer, 医学部・附属病院, 講師 (20154312)
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| Co-Investigator(Kenkyū-buntansha) |
KAKUTA Yasunori Tohoku Univ.Hospital, Research Associate, 医学部・附属病院, 助手 (80142933)
OKAYAMA Hiroshi Tohoku Univ.Hospital, Research Associate, 医学部・附属病院, 助手 (10160730)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Airway Epithelial Cells / Airway Gland Cells / Airway Smooth muscle cells / Patch clamp method / Ion channel / Membrane Potentials / IP_3 / Cyclic ADP rihore / ヒト気道 / 神経節細胞 / クロライドイオンチャンネル / カリウムイオンチャンネル / ステロイド / 気道神経節細胞 / 細胞膜電位 / 細胞内セカンドメッセンジャー / 気管支 / パッチクランプ法 / 受容体 |
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| Research Abstract |
Airways consist of epithelial cells, gland cells, smooth muscle cells and nerves. We examined the function and regulation of ion channels of these structural cells and obtained the following findings.
1) In superficial epithelium, non-ciliated cells play main role in chloride (Cl^-) secretion rather than do ciliated cells.
2) Cyclic AMP-dependent Cl^- channels (CFTR) newly develop in superficial epithelial cells in bronchitic airways.
3) In gland cells, both IP_3-sensitive and cyclic ADP ribose-sensitive Ca^<2+>-pools are necessary for initiating the activation of Cl^- channels.
4) Cholinergic stimulation induces depolarization of membrane potentials in airway smooth muscle cells, while both histamine and endothelin induce the mambrane hyperpolarization through the activation of K_<DR> channels.
5) GABA receptor-operated Cl^- channels are active in airway ganglion cells.
6) Both Na^+/K^+/Cl^- cotransporter and Na^+/K^+ pump are related to Cl^- secretion in superficial epithelial cells and gland cells.
7) Glucocorticoid suppresses the function of Cl^- channels in both superficial epithelial cells and gland cells.
8) In rabbit epithelial cells, Ca^<2+>-sensitive Cl^- channelsmainly operate, while CFTR mainly does in human superficial epithelium. PGE_2-release from human epithelium modulates both gland cell and smooth muscle cell functions, but does not in animal airways.
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