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Ion channel function and regulation of airway structural cells

Research Project

Project/Area Number 07457144
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

SHIMURA Sanae  Tohoku Univ.Hospital, Lecturer, 医学部・附属病院, 講師 (20154312)

Co-Investigator(Kenkyū-buntansha) KAKUTA Yasunori  Tohoku Univ.Hospital, Research Associate, 医学部・附属病院, 助手 (80142933)
OKAYAMA Hiroshi  Tohoku Univ.Hospital, Research Associate, 医学部・附属病院, 助手 (10160730)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsAirway Epithelial Cells / Airway Gland Cells / Airway Smooth muscle cells / Patch clamp method / Ion channel / Membrane Potentials / IP_3 / Cyclic ADP rihore / ヒト気道 / 神経節細胞 / クロライドイオンチャンネル / カリウムイオンチャンネル / ステロイド / 気道神経節細胞 / 細胞膜電位 / 細胞内セカンドメッセンジャー / 気管支 / パッチクランプ法 / 受容体
Research Abstract

Airways consist of epithelial cells, gland cells, smooth muscle cells and nerves. We examined the function and regulation of ion channels of these structural cells and obtained the following findings.
1) In superficial epithelium, non-ciliated cells play main role in chloride (Cl^-) secretion rather than do ciliated cells.
2) Cyclic AMP-dependent Cl^- channels (CFTR) newly develop in superficial epithelial cells in bronchitic airways.
3) In gland cells, both IP_3-sensitive and cyclic ADP ribose-sensitive Ca^<2+>-pools are necessary for initiating the activation of Cl^- channels.
4) Cholinergic stimulation induces depolarization of membrane potentials in airway smooth muscle cells, while both histamine and endothelin induce the mambrane hyperpolarization through the activation of K_<DR> channels.
5) GABA receptor-operated Cl^- channels are active in airway ganglion cells.
6) Both Na^+/K^+/Cl^- cotransporter and Na^+/K^+ pump are related to Cl^- secretion in superficial epithelial cells and gland cells.
7) Glucocorticoid suppresses the function of Cl^- channels in both superficial epithelial cells and gland cells.
8) In rabbit epithelial cells, Ca^<2+>-sensitive Cl^- channelsmainly operate, while CFTR mainly does in human superficial epithelium. PGE_2-release from human epithelium modulates both gland cell and smooth muscle cell functions, but does not in animal airways.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] 佐藤 正俊: "Isoprecterenol augments ATP-evoked CD-Aecretion across Canine trachel epitlelium" Respiration Physiclogy. 99. 13-18 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 長岐 雅敏: "Nitric oxide regulation of glycocnjugate gate sevretion from feline and lueran airways in vitro" Respiration Physiclogy. 102. 89-95 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 増田 徹: "Eosinoohil penatration throgy cultured luran aiwoay epithelial cell layer" American Journal of Repiratlorg cell and Moleare Bidigy. 12. 633-641 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 長岐 雅敏: "radykinen regulation of aiway submucasal gland secretion:roke of bradykinim recepter subtyse" American Journal of Repiratlorg. 270. L904-L913 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岩瀬 信久: "Sigrature currents of SO_2-induced brmdutis in rabuit" Journal of Clenical Invertigtion. 99. 1651-1661 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 岩瀬 信久: "ATP-induced CD-secretion with Na^+ aksorption in rabkit traoheul epitheliun" Respiration Physiology. 107. 173-180 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 志村 早苗: "尖症性気道疾患としての喘息の病態" メディカルトリビューン,東京, 200 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 志村 早苗: "分子生物学からみた気管支喘息" 現代医学社、東京, 450 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Satoh M,Yamaya M,Shimura S,Masuda T,Yamamoto M,Sasaki T,Sasaki H,Shirato K.: "Isoptoterenol augments ATP-evoked Cl^- secretion across canine tracheal epithelium." Respiration Physiology. 99 (1). 13-18 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagaki M,Shimura S,Irokawa T,Sasaki T,Shirato K.: "Nitric oxide regulation of glycoconjugate secretion from feline and human airways in vitro." Respiration Physiology. 102 (1). 89-95 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masuda T,Yamaya M,Shimura S,Hoshi H,Sasaki H,Shirato K.: "Eosinophil penetration through cultured human airway epithelial cell layr." American Journal of Respiratory Cell and Molecular Biology. 12 (6). 633-641 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagaki M,Shimura S,Irokawa T,Sasaki T,Oshiro T,Nara M,Kakuta Y,Shirato K.: "Bradykinin regulatio of airway submucosal gland secretion : role of bradykinin receptor subtype." American Journal of Physiology : Lung Cellular and Molecular Physiology. 270 (6 pt 1). L904-L913 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Irokawa T,Nagaki M,Shimura S,Sasaki T,Yamaya M,Yamauchi K,Shirato K.: "HMT regulates histamine-induced glycoconjugate secretion from human airways in vitro." Respiration Physiology. 108 (3). 233-240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwase N,Sasaki T,Shimura S,Hoshi T,Sasamori K,Takahashi K,Shirato K.: "Signature current of SO_<2-> induced bronchitis in rabbit." Journal of Clinical Investigation. 99 (7). 1651-1661 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwase N,Sasaki T,Shimura S,Yamamoto T,Suzuki S,Shirato K.: "ATP-induced Cl^- secretion with suppressed Na^+ absorption in rabbit tracheal epithelium." Respiration Physiology. 107 (2). 173-180 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ebihara K,Sasaki T,Hida W,Shimura S,Takasawa S,Okayama M,Shirato K.: "Role of cyclic ADP ribose in ATP-induced potassium currents in alveolar macrophages." Journal of Biological Chemistry. 272 (20). 16023-16029 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saitoh H,Okayama H,Shimura S,Fushimi T,Shirato K.: "Surfactant protein A2 gene expression by huyman submucosal glands cells." American Journal of Respiratory Cell and Molecular Bilology. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nara M,Sasaki T,Shimura S,Oshiro T,Irokawa T,Kakuta Y,Shirato K.: "Effects of histamine and endotelin-1 on membrane potentials and ion currents in bovine tracheal smooth muscle cells." American Journal of Respiratory Cell and Molecular Bilology. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Iwase N,Sasaki T,Shimura S,Irokawa T,Sasamori K,Shirato K.: "Role of non-ciliated epithelial cells in ATP-induced Cl^- secretion in rabbit tracheal epithelium" Respiration Physiology. (submitted.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sasaki T,Shimura S,Takasawa S,Nagaki M,Nara M,Okayama M,Shirato K.: "Cyclic ADP-ribose, a novel Ca^<2+>-mobilizing second messenger, operates in airway gland acinar cells in vitro." American Journal of Physiology : Lung Cellular and Molecular Physiology. (submitted.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Irokawa T,Sasaki T,Shimura S,Oshiro T,Nara M,Takishima T,Shirato K.: "Cholinomimetic action of macrolide antibiotics on airway gland electrolyte secretion." American Journal of Physiology : Lung Cellular and Molecular Physiology. (submitted.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 色川俊也: "HMY regulates histamine-induced glycoconjugate secretion from human airways in vitro" Respiration Physiology. 108. 230-240 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岩瀬信久: "Signation current of SD_2-induced bronchitis in rabbit" Journal of Clinical Investigation. 99. 1651-1661 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 岩瀬信久: "ATP-induced Cl^- secretion with suppressed Na^+ absorption in rabbit tracheal epithelium" Respiration Physiology. 107. 173-180 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 海老原 覚: "Role of cyclic ADP-ribose in ATP-induced pottassium currents in alveolar macrophagei" Journal of Biological Chemistry. 272. 16023-16029 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 斉藤弘樹: "Surfactant protein A_2 gene expression by human submucosal gland cells" American Journal of Respiratory Cell and Moleculer Biology. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 奈良正之: "Effects of histamine and endothelin-1 on membrane potentials and ion curre nts in bovine tracheal smooth muscle cells" American Journal of Respiratory Cell and Molecular Biology. (発表予定). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 長岐 雅俊: "Bradykimin regulation of airway submurcasal gland secretion : Role of bradykimin receptor subtype" Americam Journal of Physiology. 270・L14. L907-L913 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岩瀬 信久: "Signature current of SO_2-induced broiclitis in rabbit" Journal of Clinical Investigation. 99(発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岩瀬 信久: "ATP-induced Cl-secretion with suppressed Na^<2+> alssorption in rabbit tracleol epithelium" Respiration Physiology. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 岩瀬 信久: "The role of nonciliated cells in ATP-induced Cl-current across rabbit tracleal epithelium" Respiration Physiology. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 奈良 正之: "Physiolgical roles of membrane potentials and ion currents in berine tracleol smoothmuscle cells" American Journal of Physiology. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐々木 司: "Cyclic ADP-ribose, a norel Ca^<2+>-mokilizing secand messenger, operated in airway gland acilen cells in citro" Journal of Clinical Investigation. (発表予定). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 長岐雅俊: "Nitric oxide vegulation of glycoconjugate secretin from feline and human sirways in vitro" Respiration Physiology. 102. 89-95 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 佐藤正俊: "Isoproterenol augments ATP-evoked Cl^- secretion acress canine tracheal epithelium" Respiratin Physiology. 99. 13-18 (19 5)

    • Related Report
      1995 Annual Research Report
  • [Publications] 志村早苗: "平滑筋細胞" 最近医学. 50. 2139-2145 (19 5)

    • Related Report
      1995 Annual Research Report
  • [Publications] 奈良正之: "Effects of endothelin and histamine in mewbrane potentials of bovine tracheal smooth muscle cells" Am.J.Physiol,:Lung Cell.Mol.Phyeiol.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 奈良正之: "IP_3-mediated spontaneous transient outward currents in single tracheal smooth muscle" Am.J.Pespir.Cell Mol.Biol.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岩瀬信久: "ATP-induced Cl^- secretion with suppressed Na^+ alssorption in rabbit tracheal epithelium:the role of nonciliated cell" Am.J.Pespir.Cell Mol.Biol.(in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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