| Project/Area Number |
07457148
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
ABE Shosaku Sapporo Medical University, School of Medicine Third Department of Internal Medicine, Professor, 医学部, 教授 (60113510)
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| Co-Investigator(Kenkyū-buntansha) |
HIRASAWA Michio Sapporo Medical University, School of Medicine, Instructor, 医学部, 助手 (90264530)
SHIJUBO Noriharu Sapporo Medical University, School of Medicine, Assistant professor, 医学部, 助手 (70231355)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Lung cancer / Invasion / Hematogenous metastasis / Occult metastatic lesion / Cytokeratin 18 / Integrin / Catepsin B / Prognostic marker / サイトケラチン |
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| Research Abstract |
In order to detect occult metastatic lesions in non-small cell lung cancer without clinically hematogenous distant metastasis, cytokeratin positive cell in bone marrow aspirates were analyzed by immunocytochemical method using monoclonal antibody to cytokeratin 18.
Cytokeratin 18 positive cells were detected in 34/64 (53%) among non-small cell carcinoma (Stage I 32.3%, III 63.6%, IV 100%).
The alpha_2, alpha_3, alpha_5, alpha_6, integrin subunits were frequently expressed. The detection rate of cytokeratin 18-positive cells of alpha_2 integrin subunitt positive lung cancer was significantly higher than that of the alpha_2 subunit negative group. A highly significant correlation was revealed between the alpha_6beta_4 integrin subunitt and the expression of Catepsin B in lung cancer,
Integrins play very important roles as cellular adhesion molecules in occult metastatic lesion formation of lung cancer.
Especially ; alpha_2beta_1 integrin subunit may contribute to metastatic release from a primary site, migration into bloodstream and occult matastatisis formation in non-small cell carcinoma.
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