• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular immnologic studies in the pathogenesis and regulation of autoimmune demyelinating diseases

Research Project

Project/Area Number 07457155
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionShinshu University

Principal Investigator

KOH Shosei  Shinshu University Hispital, Department of Medicine (Neurology), Assistant Professor, 医学部・附属病院, 講師 (80143981)

Co-Investigator(Kenkyū-buntansha) INOUE Atsushi  Shinshu University School of Medicine, Department of Medicine (Neurology), Assoc, 医学部, 助手 (50213145)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordsmultiple sclerosis / demyelination / Theiler's virus / antibody / adhesion molecules / 実験的自己免疫性脳脊髄炎 / タイラー脳脊髄炎ウイルス / 免疫寛容
Research Abstract

We examined the role of leukocyte function-asociated antigen-1 (LFA-1) and its counter-receptor intercellular adhesion molecule-1 (ICAM-1), one of the most important pairs among adhesion molecules, in the development of Theiler's murine encephalomyelitis virus induced demyelinating disease (TMEV-IDD). Immunohistochemical study showed hyperexpression of ICAM-1 on vascular endothelial cells and LFA-1 on mononuclear infiltrating cells in spinal cord from TMEV infected mice. Treatment with anti ICAM-1 or anti LFA-1 monoclonal antibody showed suppressive effect on developement of demyelinating disease both clinically and histologically. There was downregulation in the CNS of the respective adhesion molecule after treatment. In mice treated with monoclonal antobodies to these adhesion molecules, TMEV specific delayd type hypersensitrvity and the proliferative response of T cells were decreased, and the production of TNFa and IFNg in the spleen cells was decreased and that of IL4 remained unchanged. These data suggest that ICAM-1-LFA-1 pathway is critically involved in the pathogenesis of TMEV-IDD and that antibodies to these adhesion molecules could be a novel therapeutic approach to demyelinating disease such as human multiple sclerosis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Inoue A, et al.: "Anti-adhesion molecule therapy in Theiler's murine encephalomyclitis virus-induced demyelinating disease." Internat Immunol. 9(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iwahashi T, et al.: "A study on a new antineural antibody in a case of paraneoplastic sensory neuropathy associated with breast carcinoma." J Neurol Neurosurg Psychiat,. 63. 516-519 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yahikozawa H, el al.: "Major linear antibody epitopes and capsid proteins differentially induce protective immunity against Theiler's virus-induced demyelinating disease." J Virol,. 71. 3105-3113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazaki M, et al: "Phosphatidylserine Suppresses Theiler's murine encephalomyelitis virus-induced demyelinating disease." J Neuroimmunol. 75. 113-122 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A, Koh C-S, et al.: "Detection of the soluble form of the Fas molecule in patients with multiple sclerosis and human T-lymphotropic virus type I-associated myelopathy." J Neuroimmunol. 75. 141-146 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A, Koh C-S, et al.: "The fibrin deposition in the central nervous system correlates with thedegree of Theiler's murine encephalomyelitis virus-induced demyelinating disease." J Neuroimmunol. 77. 185-194 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A, Koh C-S, et al.: "The level of tumor necrosis factor-α producing cells in the central nervous system correlates with the degree of Theiler's murine encephalomyelitis virus-induced demyelinating disease." Intermnat Immunol. 8. 1001-1008 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A, Koh C-S, et al.: "Suppression of cell-transferred experimental autoimmune encephalomyelitis in defibrinated Lewisrats." J Neuroimmunol. 71. 131-137 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hanaoka T,Fujimori M,Shingu K,Hirose S,Maruyama M,Ito K,Asanuma K,Kasuga Y,Kobayashi S,Iwahashi T,Inoue A,Koh C-S,Matsuyama I,Tsuchiya S,Amano J: "A case of occult breast cancer with paraneoplastic polyneuropathy" Breast Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koh C-S,Takahashi K,Kwaan HC: "Two-color fluorescence analysis of fibrin networks from normal and X-ray irradiated fibrinogens and incorporation of fibrinolytic components by a confocal laser-scanning microscopy" Bioimages. 5. 49-58 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Koh C-S,Yamazaki M,Ichikawa M,Isobe M,Ishihara Y,Yagita H,Kim BS: "Anti-adhesion molecule therapy in Theiler's murine encephalomyelitis virus - induced demyelinating disease" Internat.Immunol.9 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iwahashi T,Koh C-S,inoue A,Yanagisawa N: "Tumor necrosis factor-a and trnasforming growth factor-b production by mononuclear cells in the spinal cords of Lewis rats with experimental autoimmune encephalomyelitis" Tohoku J Exp.Med.183. 123-133 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakurai S,Inoue A,Koh C-S,Ohwa M,Yanagisawa N: "Soluble form of selectins in blood of patients with acute myocardial infarction and coronary intervention" Vascular Medicine. 2. 163-168 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iwahashi T,A.Inoue, Koh C-S,N.Yanagisawa: "A sutyd on a new antineural antibody in a case of paraneoplastic sensory neuropathy associated with breast carcinoma" J Neurol.Neurosurg.Psychiat.63. 516-519 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekijima Y,Tokuda T,Hashimoto T,Koh C-S,Shoji S,Yanagisawa N: "Serial magnetic resonance imaging (MRI) study of a patient with Balo's concentric sclerosis treated with immunoadsorption plasmapheresis" Multi Scler. 2. 291-294 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yahikozawa H,Inoue A,Koh C-S,Choe Y-K,Kim BS: "Major linear antibody epitopes and capsid proteins differentially induce protective immunity against Theiler's virus-induced demyelinating disease" J Virol.71. 3105-3113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazaki M,Inoue A,Koh C-S,Sakai T,Ishihara Y: "Phosphatidylserine suppresses Theiler's murine encephalomyelitis virus-induced demyelinating disease" J Neuroimmunol.75. 113-122 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Koh C-S,Sakai T,Yamazaki M,Yanagisawa N,Usuku K,Osame M: "Detection of the soluble form of the Fas molecule in patients with multiple sclerosis and human T-lymphotropic virus type I-associated myelopathy" J Neuroimmunol.75. 141-146 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Koh C-S,Yamazaki M,Yanagisawa N,Ishihara Y,Kim BS: "The fibrin deposition in the central nervous system correlates with the degree of Theiler's murine encephalomyelitis virus-induced demyelinating disease" J Neuroimmunol.77. 185-194 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazaki M,Koh C-S,Inoue A,Yanagisawa N: "Comparative study of the efficacy of plasma exchange, immunoadsorption plasmapheresis and corticosteroid administration for the treatment of Guillain-Barre syndrome" Jpn.J Apheresis.16. 204-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Tsuyusaki J,Sakai T,Koh C-S,Yanagisawa N,Arakura H,Oguchi H: "Immunoadsorption plasmapheresis in inflammatory demyelinating disease" Jpn.J Apheresis.16. 206-207 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Koh C-S,Yahikozawa H,Yanagisawa N,Yagita H,Ishihara Y,Kim BS: "The level of tumor necrosis factor-Ephi producing cells in the central nervous system correlates with the degree of Theiler's murine encephalomyelitis virus - induced demyelinating disease." Internat.Immunol.8. 1001-1008 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Koh C-S,Yanagisawa N,Taketomi T,Ishihara Y: "Suppression of Theiler's murine encephalomyelitis virus - induced demyelinating disease by administration of gangliosides" J Neuroimmunol.64. 45-53 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue A,Koh C-S,Shimada K,Yanagisawa N,Yoshimura K: "Suppression of cell-transferred experimental autoimmune encephalomyelitis in defibrinated Lewis rats" J Neuroimmunol.71. 131-137 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamazaki M,et al: "Phosphatidylserine suppresses Theiler′s murine encephalomyelitis virus-induced demyelinating disease." J Neuroimmunol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue A,Koh C-S,et al: "Detection of the soluble from of the Fas molecule in patients with multiple sclerosis and human T-1ymphotropic virus type I-associated myelopathy." J Neuroimmunol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue A,Koh C-S,et al: "The fibrin deposition in the central nervous system correlates with the degree of Theiler′s murine encephalomyelitis virus-induced demyelinating disease." J Neuroimmunol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Sekijima Y,et al: "Serial magnetic resonance imaging(MRI)study of a patient with Bolo′s concentric sclerosis treated with immunoadsorption plasmapheresis." Multiple Sclerosis. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Yahikozawa H,et al: "Major linear antibody epitopes and capsid proteins differentjally induce protective immunity against Theiler′s virus-induced demyelinating disease." J Virol. 71. 3105-3113 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamazaki M,Koh C-S,et al: "Comparative study of the efficacy of plasma exchange,immunoadsorption plasmapheresis and corticosteroid administration for the treatment of Guillain-Barre syndrome." Jpn J Apheresis. 16. 204-205 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue A,Tsuyusaki J et al: "Immunoadsorption plasmapheresis in inflammatory demyelinating disease." Jpn J Apheresis. 16. 206-207 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue A,Koh C-S,et al: "The level of tumor necrosis factor-Eφ producing cells in the central nervous system correlates with the degree of Theiler′s murine encephalomyelitis virus-induced demyelinating disease." Internat Immunol. 8. 1001-1008 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue A,Koh C-S,et al: "Suppression of Theiler′s murine encephalomyelitis virus-induced demyelinating disease by administration of gangliosides." J Neuroimmunol. 64. 45-53 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue A,Koh C-S,et al: "Suppression of cell-transferred experimental autoimmune encephalomyelitis in defibrinated Lewis rats." J Neuroimmunol. 71. 131-137 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sakurai S,et al: "Soluble form of of selectins inblood of patients with acute myocardial infarction and coronary intervention." Vascular Medicine. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Koh C-S,Inoue A,et al: "Combined therapy of corticosteroid and intermittent treatment with immunoadsorption plasmapheresis in polymyositis/dermatomyositis." Jpn J Apheresis. 14. 65-66 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Inoue,C.-S.Koh,et al: "Suppression of Theiler′s murine encephalomyelitis virus induced demyelinating disease by administration of gangliosides" Journal of Neuroimmunology. 64. 45-53 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Inoue,C.-S.Koh,et al: "The level of tumor necrosis factor-α producing cells in the spinal cord correlates with the degree of Theiler′s murine encephalomyelitis virus-induced demyelinating disease." International Immunology. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.-S.Koh,A.Inoue,et al: "Immunoadsorption plasmapheresis in Balo′s concentric sclerosis" Japanese Journal of Apheresis. 15. 42-44 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Sekijima,et al: "Serial magnetic resonance imaging(MRI)study of a patient with Balo′s concentric sclerosis treated with immunoadsorption plasmapheresis" Multiple sclerosis. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Inoue,C.-S.Koh,et al: "Immunoadsorption plasmapheresis in inflammatory demyelinating diseases" Japanese Journal of Apheresis. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] C.-S.Koh,A.Inoue,et al: "The role of immunoadsorption plasmapheresis in chronic inflammatory demyelinating polyneuropathy" Japanese Journal of Apheresis. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi