• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the role of the serotonergic neuron system in the plasticity of the developing brain

Research Project

Project/Area Number 07457181
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

TAKEUCHI Yoshihiro  Kyoto Prefectural University of Medicine Department of Pediatrics, Assistant Professor, 医学部, 講師 (80188169)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsSerotonin / Dopamine / Plasticity / Sprouting / Histochemistry / 細胞化学
Research Abstract

Serotonin (5-HT) is related to the plasticity of the brain, and 5-HT heterotypic sprouting occurs in the neostriatum of rats that suffer 6-hydroxydopamine (6-OHDA) lesioning in the neonatal period. 6-OHDA was administered to the right lateral ventricle of animals (rat and mouse) at 3 days, 7 days, 12 days and 28 days after birth. Then the animals were perfused transcardially at 2 days, 5 weeks, 10 weeks and 1 year after the administration of 6-OHDA.Immunohistochemical studies were performed with anti-tyrosine hydroxylase (TH) and anti-5-HT antibodies. NADPH-diaphorase histochemistry was also performed. Quantitative analysis on NADPH-positive cells and fiber terminals were performed using NIH Image at three coronal levels.
This study has shown that remarkable hyperinnervation of serotonin fibers occurred in the striatum of mice that had undergene the same kind of unilateral 6-OHDA lesioning at even 12 days after birth. This is an important finding as to the critical period for serotonergic heterotypic sprouting after dopamine denervation. Noteworthy is that intrinsic serotonergic heterotypic sprouting persisted throughout the time course of the study, namely, to one year after 6-OHDA lesioning, suggesting that the sprouting is long lasting. There is no significant change in the density of NADPH-positive structures in the striatum during the development of serotonergic heterotypic sprouting after dopamine-depletion. This is the first demonstration that 5-HT hyperinnervation occurs in the neostriatum of mice suffering 6-OHDA lesioning at even 12 days after birth, and that 5-HT heterotypic sprouting may be permanent.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Y.Takeuchi: "Serotonergic heterotypic sprouting in catecholamine-depleted animals:Plasticity of the striatum and the cerebral cortex." Developmental Brain Dysfunction. 9. 268-281 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takeuchi: "Serotonergic heterotypic sprouting in the developing brain." Acta Histochemica et Cytochemica. 29. 357-358 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] I.Yamazoe: "Serotonergic heterotypic sprouting in the dopamine-depleted mouse striatum-with special references to NADPH-diaphorase-positive structures." Acta Histochemica et Cytochemica. 29. 1006-1007 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takeuchi: "Thyrotropin-releasing hormone(Protirelin):Role in the treatment of epilepsy." CNS Drugs. 6. 341-350 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Wada: "Adrenoleukodystrophy induced by abnormal electro-encephalography." Acta Pediatrica Japonica. 38. 352-356 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takeuchi, A.Nishimura, I.Yamazoe, H.Matsushita, T.Sawada: "Serotonergic heterotypic sprouting in catecholamine-depleted animals : Plasticity of the striatum and the cerebral cortex" Developmental Brain Dysfunction. 9. 268-281 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takeuchi: "Serotonergic heterotypic sprouting in the developing brain." Acta Histochemica et Cytochemica. 29 (Suppl). 357-358 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] I.Yamazoe, Y.Takeuchi, A.Nishimura, H.Matsushita, S.Kawase, T.Sawada: "Serotonergic heterotypic sprouting in the dopamine-depleted mouse striatum-with special references to NADPH-diaphorase-positive structures." Acta Histochemica et Cytochemica. 29 (Suppl). 1006-1007 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Takeuchi: "Thyrotropin-releasing hormone (Protirelin) : Role in the treatment of epilepsy." CNS Drugs. 6. 341-350 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Wada, Y.Takeuchi, F.Fujiwara, I.Odani, T.Sawada: "Adrenoleukodystrophy induced by abnormal electro-encephalography." Acta Pediatrica Japonica. 38. 352-356 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Takeuchi: "Serotonergic heterotypic sprouting catecholamine-depleted animals : Plasticity of the striatum and the cerebral cortex." Developmental Brain Dysfunction. 9. 268-281 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Takeuchi: "Serotonergic heterotypic sprouting in the developing brain." Acta Histochemica et Cytochemica. 29. 357-358 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] I. Yamazoe: "Serotonergic heterotypic sprouting in the dopamine-depleted mouse striatum-with special references to NADPH-diaphorase-positive structures." Acta Histochemica et Cytochemica. 29. 1006-1007 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y. Takeuchi: "Thyrotropin-releasing hormone (Protirelin) : Role in the treatment of epilepsy." CNS Drugs. 6. 341-350 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N. Wada: "Adrenoleukodystrophy induced by abnormal electro-encephalography." Acta Pediatrica Japonica. 38. 352-356 (1996)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi