| Project/Area Number |
07457182
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kitasato University |
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Principal Investigator |
MATSUURA Nobuo Kitasato Univ.School of Medicine, Professor, 医学部・小児科, 教授 (50002332)
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| Co-Investigator(Kenkyū-buntansha) |
KAZAHARI Mayum Kitasato Univ.School of Medicine, Research Associate, 医学部, 助手 (60224383)
YOKOTA Yukifumi Kitasato Univ.School of Medicine, Assistant Professor, 医学部, 講師 (40158366)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | HLA antigen genes / DR beta genes / Mitochondrial genes / GAD antigen / DT diaphorase / Cox B virus / Autoantigens / ICA512 / HLA抗原 / IDDM(インスリン依存性糖尿病) / ミトコンドリヤ遺伝子異常 / ICA512(TA-Z) / コクサッキーBウイルス RNA / DQ抗原遺伝子 / DR抗原遺伝子 / DQβ鎖遺伝子 / DQα鎖遺伝子 / diabetogenic heterodimers / CoxBウイルスRNA |
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| Research Abstract |
1.Detection of Coxsackie B virus RNA in serum from children at the onset of IDDM
To elucidate the environmental factors of childhood IDDM,children at onset of IDDM were investigated by detection of specific Cox B sequences in serum using a transcription and a nested polymerase chain reaction (nested RT-PCR). We studied 59 cases of children with IDDM at onset, however we can't detected none of the cases until now. We are continuing to try to isolate Cox B specific virus RNA.
2.HLA-DR,DQ beta genes and DQ alpha genes analysis
We analyzed HLA antigen genes in 104 patients with IDDM and 97 controls. We found significantly decreased frequency in DRB1 150X (D), and increased in 0405 (S), 0901 (V). Moreover, 0802(D) was significantly in IDDM.DQA1 (R), DQB1 (D), DRB1 (nD) homozygotes was found most susceptibility genotype (RR=2.87) in Japanese IDDM patients.
3.Mitochondrial (Mt) gene mutations associated with Japanese childhood IDDM pat ients. : We found none of the patients had 3243 (A-G) mutations, however, 2 and 5 of the 155 IDDM patients had 3316 and 3394 mutations.
4.Long-term complications of IDDM patients and interational collaboration studies : We collaborated in DERI,DIAMOND and Hvidore study groups.
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