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Formation and localization of allergy and biomodulation by dendritic cells-cytokines and adhesion molecules-

Research Project

Project/Area Number 07457190
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKyushu University (1997)
The University of Tokyo (1995-1996)

Principal Investigator

FURUE Masutaka  Department of Dermatology, Kyushu University Professor (moved from University of Tokyo, Branch Hospital), 医学部, 教授 (70134583)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1997: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsatopic dermatitis / ECP / sELAM-1 / IL-4 / IL-5 / CCR3 / 成人型 / インターロイキン4 / イタン-ロイキン5 / sCD14 / ランゲルハンス細胞 / B7-1 / B7-2 / Th1 / Th2 / サイトカイン
Research Abstract

We have already examined 1) the serum levels of IL-4 and IL-5,2) intracellular cytokine profiles of peripheral blood mononuclear cells, and 3) chronological changes of serum markers of outgrown patients, in atopic dermatitis (AD). We have found 1) that serum levels of IL-4 and IL-5 are not useful markers for AD,2) that the flow cytometric analysis of mononuclear cells discloses a Th2-dominant pattern in AD,and 3) that serum markers such as ECP and sELAM-1 rapidly decrease in outgrown AD patients. In 1997 projects we further investigated the serum levels of ECP,sELAM-1, IL-4, IL-5 and sCD14 in order to elucidate the clinical and mutual relevance of these markers in AD,eczema and urticaria patients. The levels of ECP are elevated only in the AD patients, whereas the sE-selectin levels are higher not only in AD but also in non-atopic eczema in a severity-dependent manner. The levels of both markers significantly diminish after the treatment. Significant correlation exists between ECP levels and number of eosinophils, sE-selectin levels and itch scores, and sE-selectin levels and IgE levels. No significant changes are observed in the sCD14 and IL-4 levels (submitted for publication).
We also invetigated the expression of CCR3, one of the receptors for CC chemokines, in cultured keratinocytes as well as normal and inflammatory skins in situ. CCR3 is recentry paid attention as a Th2 marker. Keratinocytes indeed express CCR3 in a protein and mRNA level and the expression is augmented by inflammation. We are now examining the regulatory mechanism of CCR3 expression by keratinocytes. The expression of CCR3 is not detected by cutaneous dendritic cells immunohistologically.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Hironobu Ura 他: "Significance of mitotic cells or clumping cells in p53 immunopositivity of Bowen's disease." Dermatology. 194. 87-88 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kikuchi K 他: "Tissue inhibitor of metalloproteinase 1(TIMP-1)may be an autocrine growth factor in scleroderma fibroblasts." J Invest Dermatol. 108. 281-284 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naomi Yamashita 他: "Soluble E-selectin as a marker of disease activity in atopic dermatitis." J Allergy Clin lmmunol. 99. 410-416 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masutaka Furue 他: "Dysregulated expression of transforming growth factor β and its type I and type II receptors in basal cell carcinoma." Int J Cancer. 71. 505-509 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nami Yasaka 他: "Pseudoxanthomatous lesions with membranocystic changes of collagen fibers in an SLE patient receiving long-term steroid treatment." Dermatology. 194. 162-165 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okochi H 他: "Expression of tetra-spans transmembrane family(CD9,CD37,CD53,CD63,CD81,CD82)in normal and neoplastic human keratinocytes" Br J Dermatol. 137. 856-863 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hironobu Ura, Kiyoko Nashiro, Kanako Kikuchi, Sumihisa Imakado, Ataru Matsukawa, Masutaka Furue.: "Significance of mitotic cells or clumping cells in p53 immunopositivity of Bowen's disease." Dermatology. 194. 87-88 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kikuchi K,Kadono T,Furue M,Tmaki K.: "Tissue inhibitor of metalloproteinase 1 (TIMP-1) may be an autocrine growth factor in scleroderma fibroblasts." J Invest Dermatol. 108. 281-284 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naomi Yamashita, Sakae Kaneko, Osamu Kouro, Masutaka Furue, Shoso Yamamoto, Tsuyoshi Sakane.: "Soluble E-selectin as a marker of disease activity in atopic dermatitis." J Allergy Clin Immunol. 99. 410-416 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masutaka Furue, Mitsuyasu Kato, Koichiro Nkamura, Kiyoko Nashiro, Kanako Kikuchi, Hitoshi Okochi, Kohei Miyazono, Kunihiko Tamaki: "Dysregulated expression of transforming growth factor beta and its type I and type II receptors in basal cell carcinoma." Int J Cancer. 71. 505-509 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nmi Yasaka, Naoto Otake, Masutaka Furue, Kunihiko Tmaki.: "Pseudoxanthomatous lesions with membranocystic changes of collagen fibers in an SLE patient receiving long-term steroid treatment." Dermatology. 194. 162-165 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okochi H,Kato M,Nashiro K,Yoshie O,Miyazono K,Furue M.: "Expression of tetra-spans transmembrane family (CD9, CD37, CD53, CD63, CD81, CD82) in normal and neoplastic human keratinocytes-An association of CD9 with a3b1 integrin-" Br J Dermatol. 137. 856-863 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masutaka Furue: "Serum levels of IL-4 and IL-5 are not useful markers for atopic dermatitis." Jap J Dermatol. 107. 1684-1685 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hironobu Ura他: "Significance of mitotic cells or clumping cells in p53 immunopositivity of Bowen's disease." Dermatology. 194. 87-88 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kikuchi K他.: "Tissue inhibitor of metalloproteinase 1(TIMP-1)may be an autocrine growth factor in scleroderma fibroblasts." J Invest Dermatol. 108. 281-284 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naomi Yamashita他: "Soluble E-selectin as a marker of disease activity in atopic dermatitis." J Allergy Clin Immunol. 99. 410-416 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masutaka Furue他: "Dysregulated expression of transforming growth factor β and its type I and type II receptors in basal cell carcinoma." IntJ Cancer. 71. 505-509 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nami Yasaka他: "Pseudoxanthomatous lesions with membranocystic changes of collagen fibers in an SLE patient receiving long-term steroid treatment." Dermatology. 194. 162-165 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Okochi H他: "Expression of tetra-spans transmembrane family(CD9,CD37,CD53,CD63,CD81,CD82)in normal and neoplastic human keratinocytes" Br J Dermatol. 137. 856-863 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nami Yasaka,Masutaka Furue,Kunihiko Tamaki: "Expression of cutaneous lymphocyte-associated antigen defined by monoclonal antibody HECA-452 on human Langerhans cells." Journal of Dermatological Science22GD01:11. 19-27 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kunihiko Tamaki 他: "Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin." Journal of Investigative Dermatology. 106. 571-575 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masutaka Furue 他: "IL-1,but not TNF-α,synergistically up-regulates the GM-CSF-induced B7-1 expression of murine Langerhans Cells." British Journal of Dermatology. 135. 194-198 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Reiko Akasu 他: "Dermatoscopic and videomicroscopic features of melanocytic plantar nevi." The American Journal of Dermatopathology. 18. 10-18 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hironobu Ura 他: "Significance of mitotic cells or clumping cells in p53 immunopositivity of Bowen's disease." Dermatology. 194. 87-88 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masutaka Furue 他: "Dysregulated expression of transforming growth factor β and its type I and type II receptors in basal cell carcinoma." International Journal of Cancer. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatsuyoshi Kawamura Masutaka Furue: "Comparative analysis of B7-1 and B7-2 expression in Langerhans Cells: Differential regulation by Th1 and Th2 cytokines" Eur. J. Immunol. 25. 1913-1917 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Chung-Hsing Chang Masutaka Furue: "B7-1 expression of Langerhans cells is up-regulated by proinflammatory cytokines, and is down-requlated by IFN-_γ or by IL-10" Eur. J. Immunol. 25. 394-398 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Atushi Osada et al.: "Up regulation of CD44 expression by tumor necrosis factor-α is neutralized by interleukin-10 in Langerhans cells." J. Invest. Dermatol. 105. 124-127 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masutaka Furue et al.: "Epidemic Outbreak of Staphylococcal Scalded Skin Syndrome Evaluated by a Genomic DNA Fingerprinting" Lancet. 345. 1308 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masutaka Furue et al.: "Colocalization of Scavenger receptor in CD68 positive foam cells in verruciform xanthoma." J. Dermatol. Sci.10. 213-219 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nami Yasaka et al.: "CD44 expression in normal human skin and skin tumors." J. Dermatol. 22. 88-94 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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