Analysis of Abnormal Expression of Sphingomyelin Acylase in Atopic Dermatitis. Resulting in Ceramide Deficiency
| Project/Area Number |
07457192
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | TOKYO WOMEN'S MEDICAL COLLEGE |
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Principal Investigator |
KAWASHIMA Makoto TOKYO WOMEN'S MEDICAL COLEGE,DEPARTMENT OF DERMATOLOGY,PROFESSOR AND CHAIRMAN, 医学部, 教授 (60114451)
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| Co-Investigator(Kenkyū-buntansha) |
MURATA Yasuko TOKYO WOMEN'S MEDICAL COLLEGE,DEPARTMENT OF DERMATOLOGY,ASSISTANT PROFESSOR, 医学部, 助手 (40246525)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Atopic Dermatitis / Ceramide / Sphingomyelin / Sphingomyelinase / Sphingomyelin acylase / スフィンゴシルホスホリルコリン / アトピー性皮膚炎 / アシラーゼ |
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| Research Abstract |
Previously, we demonstrated that there is a marked reduction in the amount of ceramide in the stratum corneum of both lesional and nonlesional forearms in atopic dermatitis(AD), suggesting that an insufficiency of ceramides in the stratum corneum is an etiologic factor in atopic dry and barrier-disrupted skin. In this study, we investigated, as a possible mechanism involved in the ceramide deficiency, whether sphingomyelin (SM) metabolism is altered in AD as compared to normal controls. In stripped stratum corneum and biopsied whole epidermis of patients with AD.SM hydrolysis as measured at pH4.7 using *choline-methyl-^<14>C* sphingomyelin as a substrate were markedly increased by 27-and 7-fold, respectively.
Radio-thin-layr chromatography of the reaction products revealed that, whereas the SM hydrolysis in age-matched normal controls were associated with sphingomyelinase (SMase) that degrades SM to yield ceramides and phosphorylcholin (PC), most of the SM hydrolysis detected in AD were
… More
attributable not to the SMase but to a hitherto undiscovered epidermal enzyme. SM acylase, which releases free fatty acidand sphingosyl-PC (Sph-PS) instead of ceramides. The potential of this acylaselike enzyme to generate Sph-PC through SM hydrolysis was corroborated by thin-layr chromatographic analysis of the reaction products obtained using porcine kidney acylase, followed by high-perfomance liquid chromatography-mass spectrometry. Furthermore, Sph-PC was also detected by high-performance liquid chromatography-massspectrometry after incubation of SM with atopic stratum corneum samples. On the other hand, the stratum corneum of patients with contact dermatitis or chronic eczema exhibited neither increased SM hydrolysis nor the generation of Sph-PC upon radio-thin-layr chromatographic analysis. These findins suggest that SM metabolism is altered in AD,resulting in a decrease in levels of ceramides, which could be an etiologic factor in the continuous generation of atopic dry and barrier disrupted skin observed in AD. Less
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Report
(3 results)
Research Products
(11 results)
