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Development of new diagnostic imaging and therapy using radiolabeled monoclonal antibodies (^<99m>Tc, ^<186>Re)

Research Project

Project/Area Number 07457195
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionGunma University

Principal Investigator

ENDO Keigo  Gunma University Medicine, Professor, 医学部, 教授 (10115800)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1996: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1995: ¥4,000,000 (Direct Cost: ¥4,000,000)
Keywordsmonoclonal antibody / genetic engineering / humanized antibody / rhenium-186 / レニムウ-186 / テクネシウム-99m
Research Abstract

Monoclonal antibodies are expected to carry radionuclides or drugs to the specific sites, and may be used for the imaging and/or therapy of cancer, infection, myocardial infarction, thrombus and others. However monoclonal antidoeies are derived of mice and cause anti-murine autibodies in patients who received murine-monoclonal antibodies. Humanized antibodies were constructed by genetically combined with small parts of antigen-binding sites of murine antibody and the human immunoglobulin.
Two monoclonal antibodies, reactive with human ovarian cancer or human white blood cells were humanized by using the genetic engineering. Technetium-99m is an ideal radionuclide for imaging and was successfully labeled with humanized antibody. Obtained Tc-99m labeled antibody was stable in vivo and was clinically used under the guidance of the ethical committee of our institute.
Humanized antibody was safely administered to ovarian cancer patients and localized in the ovarian cancer specimens. Tc-99m labeled humanized antibody to human leucocytes was also safely administered and clearly visualized normal bone marrow.
Rehnium-186 emitts gamma-and beta-rays and is thought to be very effective for the therapy of cancers. The specific activity of Re-186 was too low to radiolabel antibodies. However, its binding to diphosphonates was stable and would be useful for the palliative therapy of bone pain caused by the metastasis of cancer.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Oriuchi,N.: "Different biodistribution of ^<99m>Tc-labelled chimeric mouse-human monoclonal antibody between athymic mice model and human." British J.of Cancer. 73. 1466-1472 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe,N.: "Indirect labeling of macroaggregated albumin with indium-111 via diethylenetriaminepentaacetic acid." Nucl.Med.& Biology. 23. 595-598 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iida,H.: "A multicenter validation of regional cerebral blood flow quantitation using 〔^<123>I〕 Iodoamphetamine and single photon emission computed tomography." J.Cereb. Blood Flow. Metab.16. 781-793 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,T: "Contrast enhancement of PC-3 prostate cancer for magnetic resonance imagiang : Animal studies using tumor-localizing MN-metalloporphyrin (the-MN-ASP)." J.Urology. 156. 1850-1852 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tateno,M.: "Assessment of fatty acid uptake in ischemic heart disease without myocardial infarction." J.Nucl.Med.37. 1981-1985 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirano,T.: "Differentiating histologic malignancy of primary brain tumors : Pentavalent technetium-99m-DMSA." J.Nucl.Med.38. 28-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 遠藤啓吾、中村仁信: "癌の新しい画像診断" 医薬ジャーナル社, 245 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oriuchi, N.Watanabe, N., Sugiyama, S., Higuchi, T., Imai, K., Yamanaka, H., Hashimoto, M., Kanda, H., Endo, K.: "Different biodistribution of ^<99m>Tc-labeled chimeric mouse-human monoclonal antibody between athymic mice model and human." British J Cancer. 73. 1466-1472 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watanabe, N., Shirakami, Y., Tomiyoshi, K., Oriuchi, N., Hirano, T., Yukihiro, M., Inoue, T., Endo, K.: "Indirect labeling of macroaggregated albumin with indium-111 via diethylenetriaminepentaacetic acid." Nucl.Med.& Biology. 23. 595-598 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iida, H., Akutsu, T., Endo.K., Fukuda, H., Inoue, T., Ito, H., Koga, S., Komatani, A., Kuwabara, Y., Momose, T., Nishizawa, S., Odano, I., Ohkubo, M., Sasaki, Y., Suzuki, H., Tanada, S., Toyama, H., Yonekura, Y., Yoshida, T.Uemura, K.: "A multicenter validation of regional cerebral blood flow quantitation using [123I] iodoamphetamine and single photo n emission computed tomography." J.Cereb.Blood Flow.Metab.16. 781-793 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, T., Nakano, K., Tomiyoshi, K., Sakata, I., Endo, K., Yamanaka, H.: "Contrast enhancement of PC-3 prostate cancer for magnetic resonance imaging : Animal studies using tumor localizing MN-metallo porphyrin (the-MN-ASP)." J.Urology. 156. 1850-1852 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taneno, M., Tamaki, N., Yukihiro, M., Kudoh, T., Hattori, N., Tadamura, E., Nohara, R., Suzuki, T., Endo, K., Konishi, J.: "Assessment of fatty acid up take in ischemic heart disease without myocardial infarction." J.Nucl.Med.37. 1981-1985 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirano, T., Otake, H., Shibasaki, T., Tamura, M., Endo, K.: "Differentiating histologic malignancy of primary brain tumors : Pentavalent technetium-99m-DMSA." J.Nucl.Med.38. 28-26 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oriuchi,N.: "Different biodistribution of ^<99m>Tc-labelled chimeric mouse-human monoclonal antibody between athymic mice model and human." British J.of Cancer. 73. 1466-1472 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Watanabe,N.: "Indirect labeling of macroaggregated albumin with indium-111 via diethylenetriaminepentaacetic acid." Nucl.Med.& Biology. 23. 595-598 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iida,H.: "A multicenter validation of regional cerebral blood flow quantitation using [^<123>I] Iodoamphetamine and single photon emission computed tomography." J.Cereb.Blood Flow.Metab.16. 781-793 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,T.: "Contrast enhancement of PC-3 prostate cancer for magnetic resonance imagiang : Animal studies using tumor-localizing MN-metalloporphyrin (the-MN-ASP)." J.Urology. 156. 1850-1852 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tateno,M.: "Assessment of fatty acid uptake in ischemic heart disease without myocardial infarction." J.Nucl.Med.37. 1981-1985 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirano,T.: "Differentiting histologic malignancy of primary brain tumors : Pentavalent technetium-99m-DMSA." J.Nucl.Med.38. 28-26 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 遠藤啓吾、中村仁信: "癌の新しい画像診断" 医薬ジャーナル社, 245 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Oriuchi, N., Endo, K.: "Semiquantitative SPECT tumor uptake of technetium-99m-labeled anti-CEA monoclonal antibody in colorectal tumor." J. Nucl. Med.36. 679-683 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe, N., Endo, K.: "Expression of C-terminal src kinase in human colorectal cancer cell lines." Jpn. J. Clin. Oncol.25. 5-9 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hosono, M., Endo, K.: "Kinetics of platelets in dogs with thrombocytopenia induced by antiglycopreotein IIb/IIIa receptor monoclonal antibody." Nucl. Med. Biol.22. 71-76 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sato, N., Endo, K.: "Origin of posterior pituitary high intensity on T1-weigthed magnetic resonance imaging : Immunohistochemical, electron microscopic, and magnetic resonance studies of posterior pituitary lobe of dehydrated rabbits." Investive Radiology. 30. 567-571 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi, H., Endo, K.: "Inflammation-seeking scintigraphy with radiolabeled biotinylated polyclonal IgG followed by the injection of avidin chase." Nucl. Med. Biology. 23. 29-32 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Oriuchi, N., Endo, K.: "Independent thallium-201 accumulation and F-18-fluorodeoxygulcose metabolism in glioma." J. Nucl. Med.(in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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