| Project/Area Number |
07457208
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Mie University |
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Principal Investigator |
KOMORI Teruhisa Faculty of Medicine, Mie University, Assistant, 医学部, 助手 (40178380)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | brain cytokines / interleukin-6 / interleukin-6 receptor / PCR / stress / neurotransmitter / カテコールアミソニューロン / サイトカイン |
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| Research Abstract |
Male Wistar rats were restrained for 4 hours or at 4 hours/day for 2 or 3 days. The rats were decapitated 30 minutes after the restraint and the hypothalamus and midbrain were dissected. The interleukin (IL) -6 receptor (R) mRNA levels were determined by a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method.
The IL-6R message in the both regions was significantly decreased in the 4-hour restrained rats. The repetition of restraint generally induced a further decrease of the IL-6R message. Additional experiments revealed that such a reduction was also induced by the short-term, such as 30-minute restraint and lasted more than several hours after the restraint.
Using the same method, we are studying changes of IL-6 message and the messages of various neurotransmitter receptors. In the case of short-term restraint, the IL-6 message could be increased after a transient decrease.
These results may be consistent with various actions of IL-6, such as neurotrophic action and the activation of hypothalamopituitary adrenal axis. We plan to clarify the actions of brain cytokines in more detail.
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