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Structural and functional analysis of phenotypic modulation of vascular smooth muscle cell

Research Project

Project/Area Number 07457216
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionChiba University

Principal Investigator

SAITO Yasushi  Chiba University School of Medicine Professor, 医学部, 教授 (50101358)

Co-Investigator(Kenkyū-buntansha) MORI Seijiro  Chiba University School of Medicine Assistant, 医学部, 助手 (50270848)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordsvascular smooth muscle cell / cell growth / cell migration / 血管平滑筋細胞 / 形質転換 / 血小板由来増殖因子 / 動脈硬化症
Research Abstract

The purpose of this study is to investigate the mechanisms for the phenotypic modulation of vascular smooth muscle cells, which is implicated in the development of atherosclerosis. The study was initiated by our previous observation that the intimal smooth muscle cells show amplified growth potential as well as migratory activity, and they efficiently incorporate the denatured lipoprotein to become foam cells like macrophages. In the present study, we have found that type IV collagen treatment of endothelial cells suppresses the expression of vascular cell adhesion molecule-1, suggesting that vascular smooth muscle cells can change the phenotype of the endothelial cells by secretion of extracellular matrices and such phenomenon plays some role in the developmental process of atherosclerosis. Next, we found that TGF-beta1 administration enhances intimal thickening of the legions made by ballon catheter injury in rabbits, indicating that TGF-beta produced by various cells in atherosclerotic legions accelerates the progression of atherosclerosis possible by promoting the secretion of extracellular matrices from the vascular smooth muscle cells. Finally, we also found that focal adhesion kinase plays an important role in the intracellular signal transduction for chemotaxis stimulated by platelet-derived growth factor downstream of phosphatidylinositol 3-kinase. The findings will reveal the molecular mechanism of the chemotactic signaling inside the cells in response to various extracellular stimuli.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 斎藤 康: "Mechanism of phenotype formation of smooth muscle cells" Annals New York Acad.Sci.748. 7-11 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 森崎 信尋: "Specific inhibition of vascular cell adhesion molecule-l expression by type IV collagen in endothelial cells" Biochem.Biophys.Res.Commun.214. 1163-1167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 神崎 哲人: "In vivo effect of TGF-β 1 : enhanced intimal thickening by administration of TGF-β 1 in rabbit arteries injured with a balloon catheter" Arterioscler.Thromb.Vasc.Biol.15. 1951-1957 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 斎藤 雄司: "Phosphatidylinositol 3-kinase activity is required for the activation process of focal adhesion kinase by platelet-derived growth factor" Biochem.Biophys.Res.Commun.224. 23-26 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasushi Saito: "Mechanism of phenotype formation of smooth muscle cells." Annals New York Acad.Sci.748. 7-11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nobuhiro Morisaki: "Specific inhibition of vascullar cell adhesion molecule-1 expression by type IV collagen in endothelial cells." Biochem.Biophys.Res.Commun.214. 1163-1167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuto Kanzaki: "In vivo effect of TGF-beta1 : enhanced intimal thickening by administration of TGF-beta1 in rabbit arteries injured with a ballon catheter" Arterioscler.Thromb.Vasc.Biol.15. 1951-1957 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yuji Saito: "Phosphatidylinositol 3-kinase activity is required for the activation process of focal adhesion kinase by platelet-derived growth factor" Biochem.Biophys.Res.Commun.224. 23-26 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 斎藤康: "Mechanism of phenotype formation of smooth muscle cells" Annals New York Acad. Sci.748. 7-11 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森崎信尋: "Specific inhibition of vascular cell adhesion molecule-1 expression by type IV collagen in endothelial cells" Biochem. Biophys. Res. Commun.214. 1163-1167 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 神崎哲人: "In vivo effect of TGF-β 1 : enhanced intimal thickening by administration of TGF-β1 in rabbit arteries injured with a balloon catheter" Arterioscler. Thromb. Vasc. Biol.15. 1951-1957 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 斎藤雄司: "Phosphatidylinositol 3-kinase activity is required for the activation process of focal adhesion kinase by platelet-derived growth factor" Biochem. Biophys. Res. Commun.224. 23-26 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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