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Cellular Physiology and molecular biology of amylin in NIDDM

Research Project

Project/Area Number 07457226
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内分泌・代謝学
Research InstitutionMiyazaki Medical College

Principal Investigator

MATSUKURA Shigeru  Miyazaki Medical College, Faculty of Medicine, Professor, 医学部, 教授 (70030939)

Co-Investigator(Kenkyū-buntansha) NAKAZATO Masamitsu  Miyazaki Medical College, Faculty of Medicine, Full-time instractor, 医学部, 講師 (10180267)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsamylin / IAPP / NIDDM / islet amyloid / 糖尿病
Research Abstract

Amylin, also designated islet amyloid polypeptide, is a 37-amino acid peptide that was isolated from islet amyloid in 1987. The peptide is thought to be involved in the etiology of NIDDM by opposing insulin action in peripheral tissues and by destroying islet construction through amyloid deposition. We studied the properties of amylin, its synthesis and secretion in abnormal glucose tolerance, and amyloidogenesis of the islet in NIDDM.
We developed three RIAs for amylin : human and rat common N-terminal region of amylin, and both human and rat C-terminal amylin. We also identified endogenous molecular forms of amylin in pancreata, gastrointestinal tracts and plasma of many mammals. Amylin messenger RNA whose size is 900 bases is predminantly expressed in the pancreas. Amylin is detected in rat fetal pancreas, and its content gradually increases during development. Amylin in the antrum appears 3 days after birth. In the human pancreas, amylin is detected at 15 weeks of gestation. Pancreatic amylin content was 221.6<plus-minus>78.2 pmol/g wet weight in man and 328.5<plus-minus>25.0 pmol/g wet weight in rat, being approximately 1-2% of insulin content. Basal plasma immunoreactive amylin level in normal individuals was 2.4-5.8 fmol/ml, being increased in subjects with ovesity or impaired glucose tolerance, and decreased in diabetic patients. Amylin was co-secreted with insulin in physiological conditi
Islet amyloid deposition is not responsible for the primary etiology of NIDDM,however, the amyloid deposits could intensify islet dysfunction and contribute to the progression of NIDDM.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 中里雅光,松倉 茂: "アミリンの遺伝子構造とII型糖尿病の成因における病態生理学的意義" 病理と臨床. 14. 1474-1479 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nakazato,S.Matsukura: "Proceeding on Intrnational workshop on Amylin Assay Methodology" Amylin Pharmacluticals, 402 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nakazato, S.Matsukura: "Highly sensitive radioimmunoassay for mammalian amylin" Proceeding on International workshop on Amylin Assay Methodology. 42-45 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中里雅光,松倉茂: "アミリンの遺伝子構造とII型糖尿病の成因における病態生理学的意義" 病理と臨床. 14. 1474-1479 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Nakazato,S.Matsukura: "Proceeding on International workshop on Amylin Assay Methodology" Amylin Pharmacluticals, 402 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] miyazato,m.,Nakazato,m.Shiomi,K.,Kangawa,K,matsuo,H,matsukura,S.: "Isolation and sequence determination of two novel N-terminal fragments of islet amyloid peptide in rat pancreas" Regul.Pept.49. 203-210 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宮里 幹也、中里雅光、松倉 茂: "糖尿病とアミロイド" BIO Clinica. 9. 36-40 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 塩見一剛、中里雅光、松倉 茂、松尾壽之: "老化と生理活性ペプチド,Advances in Aging and Health Research" 長寿科学振興財団, 11 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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