• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cellular and molecular anaylsis of glomerular and tubular functions in hypertension

Research Project

Project/Area Number 07457237
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionTohoku University

Principal Investigator

ABE Keishi  Tohoku Univ.2nd.Dept.Int.Med., Prof., 医学部, 教授 (60004777)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Kazuhisa  School of Medicine, Tohoku University, ., 医学部, 助手 (40260426)
ITO Sadayoshi  School of Medicine, Tohoku University, ., 医学部, 講師 (40271613)
OMATA Ken  Tohoku Univ.2nd.Dept.Int.Med., assist Prof., 医学部・付属病院, 講師 (50194634)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1995: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordsglomerular afferent arteriole / microperfusion / 20-HETE / calcium antagonist / thromboxane / prostaglandin / cAMP / EP_3 receptors / 輸入細動脈 / 輸出細動脈 / 一酸化窒素 / プロスタグランディン / 糸球体 / 微小灌流 / 腎不全 / チトクロームP450 / トロンボキサン / EP_<3B>受容体
Research Abstract

Glomerular and tubular functions are important for homeostasis of body fluid and electrolytes, and hence for the regulation of systemic blood pressure. In this project we employed an in vitro preparation in which microdissected afferent arterioles, a vascular segment crucial to glomerular hemodynamics, are perfused at a controlled pressuse. In addition, the role of prostaglandins were studied using various molecular and cellular biological approaches.
1) We studied the effect of 20-hydroxyeicosatetraenoic acid (20-HETE) in isolated microperfused rabbit afferent arterioles. We found that elevated vasal tone is required for 20-HETE to exert its vasoconstrictor action, which is endothelium-dependent and is partially mediated by cyclooxygenase products. In SHR,elevated renal 20-HETE production and increased afferent arteriolar tone co-exist during development of hypertension, with their synergistic actions contributing to the pathogenesis of hypertension.
2) The calcium antagonist is most wi … More dely used for treatment of hypertension. We found that calcium antagonists dilate afferent arterioles preconstricted with norepinephrine. Such dilation would render glomerular capillary pressure dependent on systemic blood pressure. Thus, in order for calcium antagonists to exert renoprotective effect by lowing glomerular capillary pressure, systemic blood pressure should be well controlled.
3) Studies with microdissected nephron segments have demonstrated that PGs and produed mainly in the vasculature including the glomerulus and collecting tubules. We cloned cDNA of the EP3 receptors, subtype of PGE2 receptor, from the rat kidney, and analyzed its intrarenal distribution and signal transduction mechanism. We found that mRNA of the EP3 receptors was expressed in the distal nephron, including the thick ascending limb and cortical collecting ducts, while their subtype EP3A and EP3B receptors were coupled to a decrease in intracellular cAMP and an increase in Ca2+ respectively, both of which are known to inhibit tubular transport. Thus the PG system appears to function at the site or vicinity of production, controlling water and electrolyte balances. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Takahashi N.,et al.: "Immunohistochemical localization of rat thromboxane receptor and synthase in rat tesis." Endocrinology. 136. 4143-4146 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N.,et al.: "Immunohistochemical localization of thromboxane receptor and synthase in rat kidney." J.Am.Soci.Nephrol.6. 762 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Abe T.,et al.: "Rat kidney thromboxane receptor : molecular cloning,signal transduction,and intrarenal expression localization." Hypertension. 27. 781-785 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arima S.,et al.: "Diverse effects of calcium antagonists on glomerular hemodynamics." Kidney Int.49(S)55. S132-S134 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arima S.,et al.: "Platelet-activating factor dilates efferent arterioles viaglomerulus-derived nitric oxide." J.Am.Soci.Nephrol.7. 90-96 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S.,et al.: "Influence of anti-hypertensive drugs on glomerular hemodynamics." Jpn.J.Nephrol.38. 115-118 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S.,et al.: "Tubuloglomerular feedback." Jpn.Heart J.37. 153-163 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S.,et al.: "Mitric oxide in the juxtaglomerular apparatus." Kidney Int.49(S)55. S6-S8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Omata K.,et al.: "Therapeutic advantages of angiotensin converting enzyme inhibitors in chronic renal disease." Kidney Int.49(S)55. S57-S62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi K.,et al.: "Immunocalization of rat thromboxane receptor in the kidney." Endocrinology. 137. 5170-5173 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi K.,et.al.: "Functional analysis and chromosomal gene assigment of rat kidney prostaglandin EP_3 receptor." Kidney Int.49(S)55. S183-S186 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi K.,et al.: "Assignment of the gene for rat thromboxane receptor(Tbxa2r)to chromosome 7q11 by fluorescence in situ hydridization." Cytogenet.Cell.Genet.73. 79-80 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S.,et al.: "Role of nitric oxide in the control of glomerular circulation." Clin.Exp.Pharmacol.Physiol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S.,et al.: "Contractile properties of afferent and efferent arterioles." Clin.Exp.Pharmacol.Physiol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N., et al: "Immunohistochmical localization of rat thromboxane receptor and synthase in rat tesis." Endocrinology. 136. 4143-4146 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N., et al: "Immunohistochemical localization of thromboxane receptor and synthase in rat kidney." J.Am.Soci.Nephrol.6. 762 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Abe T., et al: "Rat kidney thromboxane receptor : molecular cloning, signal transduction, and intrarenal expression localization." Hypertension. 27. 781-785 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arima S., et al: "Diverse effects of calcium antagonists on glomerular hemodynamics." Kidney Int.49 (Suppl.55). S132-S134 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arima S., et al: "Platelet-activating factor dilates efferent arterioles via glomerulus-derived nitric oxide." J.Am.Soci.Nephrol.7. 90-96 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S., et al: "Influence of anti-hypertensive drugs on glomerular hemodynamics." Jpn.J.Nephrol.38. 115-118 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S., et al: "Tubuloglomerular feedback." Jpn.Heart J.37. 153-163

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S., et al: "Nitric oxide in the juxtaglomerular apparatus." Kidney Int.49(Suppl.55). S6-S8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Omata K., et al: "Therapeutic advantages of angiotensin converting enzyme inhibitors in chronic renal disease." Kidney Int.49(Suppl.55). S57-S62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi K., et al: "Immunocalization of rat thromboxane receptor in the kidney." Endocrinology. 137. 5170-5173 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi K., et al: "Functional analysis and chromosomal gene assigment of rat kidney prostaglandin EP_3 receptor." Kidney Int.49(Suppl.55). S183-S186 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeuchi K., et al: "Assignment of the gene for rat thromboxane receptor (Tbxa2r) to chromosome 7q11 by fluorescence in situ hydridization." Cytogenet.Cell.Genet.73. 79-80 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S., et al: "Role of nitric oxide in the control of glomerular circulation." Clin.Exp.Pharmacol.Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito S., et al: "Contractile properties of afferent and efferent arterioles." Clin.Exp.Pharmacol.Physiol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Arima S, Ito S, Omata K, Abe K, et al.: "Diverse effects of calcium antagonists on glomerular hemodynamics." Kidney Int.49 (suppl. 55). S132-S134 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Arima S, Ren Y, Ito S, et al.: "Platelet-activating factor dilates efferent arterioles via glomerulus-derived nitric oxide." J. Am. Soc. Nephrol.7. 90-96 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Abe K.: "Influence of anti-hypertensive drubgs on glomerular hemodynamics." Jpn. J. Nephrol.38. 115-118 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Abe K.: "Tubuloglomerular feedback." Jpn. Heart J.37. 153-163 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Carretero OA, Abe K.: "Nitric oxide in the juxtaglomerular apparatus." Kidney Int.49 (suppl. 55). S6-S8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Omata K, Kanazawa M, Abe K, et al.: "Therapeutic advantages of angiotensin converting enzyme inhibitors in chronic renal disease." Kidney Int.49 (suppl. 55). S57-S62 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahashi N, Takeuchi K, Abe K, et al.: "Immunolocalization of rat thromboxane receptor in the kidney." Endocrinology. 137. 5170-5173 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeuchi K, Takahashi N, Abe K, et al.: "Functional analysis and chromosomal gene assignment of rat kidney prostaglandin EP_3 receptor." Kidney Int.49 (suppl. 55). S183-S186 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeuchi K, Nakagawa K, Abe K, et al.: "Assignment of the gene for rat thromboxane receptor (Tbxa2r) to chromosome 7q11 byfluorescence in situ hybridization." Cytogenet. Cell Genet.73. 79-80 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Carretero OA, Abe K.: "Role of nitric oxide in the control of glomerular circulation." Clin. Exp. Pharmacol. Physiol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ito S, Abe K.: "Contractile properties of afferent and efferent arterioles." Clin. Exp. Pharmacol. Physiol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahashi N,et al.: "Immunohistochemical localization of rat thromboxane receptor and synthase in rat tesis." Endocrinology.136. 4143-4146 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takahashi N,et al.: "Immunohistochemical localization of thromboxane receptor and synthase in rat kidney." J.Amer.Soc.Nephrol.6. 762 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Abe T,et al.: "Rat kidney thromboxane receptor:molecular cloning,signal transduction,and intrarenal expression localization." J.Clin.Invest.96. 675-664 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] ArimaS, et al.: "20-HETE requires increased vascular tone to constrict rabbit afferent arterioles." Hypertension(1996年3月号). (掲載予定). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takauchi K, et al.: "Assignment of the rat thromboxane receptor gene to 7q11 by in situ hybridization." Cytogenet.Cell Genet.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takeuchi K, et al.: "Functinal analysis and chromosomal gene assignment of rat prostaglandin EP_3 receptor." Kidney Int.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Abe T, et al: "Intrarenal expression of MDCK-type chloride channel." Nephron.(in press). (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi