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Constrution of cDNA bank from bispsy speeinens for unltiple gene analyis of cancer

Research Project

Project/Area Number 07457259
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionYOKOHAMA CITY UNIVERSITY

Principal Investigator

SIMADA Hirosi  Yokohama City Univ. , School of Medicine, Professor, 医学部・第2外科, 教授 (90117747)

Co-Investigator(Kenkyū-buntansha) ICHIKAWA Yasusi  Yokohama City Univ. , School of Medicine, Assistant, 医学部・第2外科, 助手 (70254208)
YAMAOKA Hiroyuki  Yokohama City Univ. , School of Medicine, Lecture, 医学部・第2外科, 講師 (90230317)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥5,200,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
KeywordscDNA Bank / mRNA / Cancer / 分子生物学 / メッセンジャーRNA
Research Abstract

The "Cancer cDNA Bank" of colorectal cancer patients was constructed with our unique mRNA assay system.
Total nucleic acids extracted from colorectal cancers were applied to 96-well plates, where poly-d (T) sequences of oligonucleotides were immobilized. After hybridization, the ds-cDNA was synthesized on the plate using the captured mRNA as a template. The resultant sense cDNA removed from the plate was used for RT-PCR for analysis of various genes. The sense strand of the cDNA was repeatedly synthesized using the immobilized antisense cDNA as a template even after storage.
Furthermore, the results of RT-PCR can be easily compared among different specimens, if the same amount of total mRNA was applied to the plate for the ds-cDNA synthesis.
These demonstrated that the "Cancer cDNA Bank" constructed from clinical materials provides almost unlimited supplies of cDNA for multiple gene analysis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] TAKASHI ISHIKAWA et al: "Construction of cDNA Bank from bcopsy specimen for multcple gene analysis of Cancer" Clinical Chemistry. 43. in printing (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YASUHIRO MIURA et al: "Fluorowetric determination of total mRNA with olyod(T)immobilized on microtiter plates" Clinical Chemistry. 42. 1758-1764 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YASUSHI ICHIKAWA et al: "Clinical application of a novel DNA fluorescence intecalator pyryliun dye,for the quantitation of PCR products" Seam J Clin.Lab.Invest.56. 641-647 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] TAKASHI ISHIKAWA et al: "Maticlysin is associated with progression of colorectal cancer" Cancer Letters. 107. 5-10 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YASUSHI ICHIKAWA et al: "Laboratory cliaguozis of helicobactor pyroli infector by polymerase chain reactin" Res.Com.in Mol.Path.and Pheun.91. 117-127 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石川孝他: "大腸癌肝転移の機序と対策-遺伝子治療をめざして-" 日本消化器外科学会雑誌. 29. 878-883 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T Ishikawa, Y Ichikawa, M Miura, et al.: "Construction of cDNA Bank from biopsy specimens for multiple gene analysis of cancer." Clinical Chemistry. 43 : 5. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T Ishikawa, Y Ichikawa, M Mitsuhashi, et al.: "Matrilysin is associated with progression of colorectal tumor." Cancer Letters. 107. 5-10 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Miura, Y Ichikawa, T Ishikawa, et al.: "Fluorometric detemination of total mRNA with oligo (dT) immobilized on microtiter plates." Clinical Chemistry. 42 : 11. 1758-1764 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Ichikawa, M Mitsuhashi, T Ishikawa, et al: "Laboratory diagnosis of helicobactor pyroli infection by polymerase chain reaction." Res Com in Mol Path Pharm. 91 : 1. 117-127 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Ichikawa, T Ishikawa, N Momiyama, et al.: "Clinical application of a novel DNA fluorescence intercalater, pyrylium dye, for the quantitation of PCR products." Scand J Clin Lab Invest. 56. 641-647 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T Ishikawa, Y Ichikawa, N Momiyama, et al.: "Strategy for the possible microscopic liver metastasis of colorectal cancer -For the furture gene therapy for colorectal cancer-" J J Gastro Surg. (29). 878-883 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H Shimada, Y Ichikawa, T Ishikawa, et al.: "The construction of cancer cDNA bank." Gantiryou no ayumi. (15). 33-41 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] YASUHIRO MIURA et al: "Fluorometric determiuation of total mRNA with oligo d(T) immobilized on mierotiter plates" Clinical Chemistry. 42. 1758-1764 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] TAKASHI ISHIKAWA et al: "Construction of cDNA Bank from biosy specimens for instltiple gene analysis of cencer" Clinical Chemstry. (in printing).

    • Related Report
      1996 Annual Research Report
  • [Publications] YASUSHI ICHIKAWA et al: "Clinical application of a novel DNA flnorescence intercalator,pyrylim dye,for the quomtitation of PCR products" Sean J Clin.Lab.Invest.56. 641-647 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] TAKASHI ISHIKAWA et al: "Matrilysin is associtated with progression of colorectal cancer" Cancer Letters. 107. 5-10 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石川孝: "大腸癌肝転移の機序と対策-遺伝子治療をめざして" 日本消化器外科学会誌. 29. (1998)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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