| Project/Area Number |
07457275
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kobe University |
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Principal Investigator |
YAMAMOTO Masahiro Kobe University, School of Medicine, Associate Professor, 医学部, 助教授 (40166822)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIDA Hidefumi Kobe University, Kobe University Hospital, Medical Staff, 医学部・附属病院, 医員
OHASHI Osamu Kobe University, Kobe University Hospital, Medical Staff, 医学部・附属病院, 医員
齋藤 洋一 神戸大学, 医学部, 教授 (90004803)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | Pancreatic cancer / Cancer cell invasion / Growth factor / Chemoattactant |
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| Research Abstract |
We have investigated the biological characterristics in the development of the metastasis of the carcinoma of the pancreas. 1) The c-K-ras and c-fos gene expression were increased in the surgical specimen of the pancreatic cancer. 2) The CD44 cell surface antigen was highly expressed in human pancreatic adenocarcinoma cells. The invasive ability of the cells was suppressed by anti-CD44 antibody. The CD-44 molecules plays an important role in pancreatic cancer cell invasion of the basement membrane. 3) The critical involvement of the syalyl Lewis A antigen (SLe^2) and the legand of endotherial leukocyte adhesion molecule-1(ELAM-1) was demonstrated in human pancreatic cancer cell vasoinvasion. The invasion of pancretic cancer cells to the endothelial cell layr was greatly suppressed by anti-SLe^2 or anti-ELAM-1 antibody. 4) The high gradient of basic fibroblast growth factor (b-FGF) increased the invasive ability of the pancreatic cancer cells, and the invasiveness of the cells was suppressed by anti-FGF receptor antibody. 5) Hepatocyte growth facter (HGF) and its receptor/c-MET regulate both cell growth and invasion of human pancreatic cancer. Dose-dependently HGF promoted both in the pancreatic cancer cells that expressed c-MET and as a chemoattractant the high gradient of HGF determined the direction of the invasiveness of the cells. 6) The expreriments using two hamster pancreatic carcinoma cell lines demonstrated the complex activities of tissue- and serum-selective growth or migration properties relating to organ preferential metastasis of pancreatic carcinoma cells.
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