• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Experimental studies of small bowel transplantation using newborn intestines.

Research Project

Project/Area Number 07457279
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionJichi Medical School

Principal Investigator

MIYATA Michio.MD.PhD.  Jichi Medical School, Department of Surgery Professor, 医学部, 教授 (90048976)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Eiji.MD.PhD.  Jichi Medical School, Department of Clinical Pharmacology Associate Professor, 医学部, 助教授 (00245044)
清崎 浩一  自治医科大学, 医学部, 助手 (80265280)
遠山 信幸  自治医科大学, 医学部, 助手 (10265283)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Keywordssmall bowel transplantation / newborn / rejection / GVHD / neovasculization / 血管新生 / 新生仔ドナー / GVHD / 消化管運動
Research Abstract

Using the characters that the newborn organs are immunologically immatured, we studies exprimental small bowel transplantation (SBT) using newborn intestines in rats.
In a singeneic combination, newborn grafts (intestine, spleen, liver, kidney, heart and pancreas) were transplanted into the subcutaneous space. Intestinal grafts obtained from the different-time newborns were also tested from the point of neovasculization rate. To show the absorbable ability of newborn intesting, the recipients were totally reconstructed by use of the newborn intestine grafted at the greater omentum. The grafts were assessed morphologically, histologically, electrophysiologically, and nutritionally. The rate of neovasculization in spleen, intestine, kidney, and heart was 87.5,70.6,41.7 and 20.0%, respectively. However, no neovasculization was observed in liver nor in pancreas. The ability of revasculization was rapidly lost after birth, and non using the newborn surviving longer than 11 days. The intestinal graft transplanted at the greater omentum showed normal EEG wave and could be reconstructed, resulting in the growth of the recipient.
In an allogeneic combination of LEW-into-PVG/c, a short course of 15-Deoxyspergualin prolonged N-SBT.Furthermore, splenectomy and/or thrmectomy on the recipients were efficient on graft survival. In a xenogeneic combination of B6-into-LEW,cyclophosphamide plus tacrolimus were effective on the graft survival, but histologically impaired. However, additional treatment of hepatic lymphomyeloid cell infusion could improved histologically.
On the contrary, SBT using the matured intestine showed sever rejection reaction in the acute and chronic phase. A new imunosuppressant, FTY720 was effective on combination therapy with conventional immunosuppressive drugs.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Toyama N: "Fulminant second-set allograft rejection and endoscopic findings following small bowel transplantation in the rat." J Gastroenterology. 30. 465 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kiyozaki H: "Segmental small bowel transplantation in the rat:Comparison of lipid absorption between jejunal and ileal grafts." JPEN. 20. 67 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi E: "Comparison of graft rejection between visualized or newborn intestinal transplantation in the rat." Transplant Proc. 28. 2485 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saito T: "Lipid absorption of the allointestinal grafts under a short course of methyldeoxysupergualin." Transplant Proc. 28. 2570 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitusada M: "Prevention of graft rejection and graft-versus-host reaction by novel immunosuppressant,FTY720,in rat samll bowel transplantation." Transplant Int. 10. 343 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saito T: "Endoscopic findings in transplanted allo-intestine of rats after discontinuance of immunosuppressive agents." J Gastroenterology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyama N,et al.: "Fulminant second-set allograft rejection and endoscopic findings following small bowel transplanation in the rat." J Gastroenterology. 30. 465 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kiyozaki H,et al.: "Segmental small bowel transplantation in the rat : Comparison of lipid absorption between jejunal and ileal grafts." JPEN. 20. 67 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi E,et al.: "Comparison of graft rejection between visualized or newborn intestinal transplantation in the rat." Transplant Proc. 28. 2485 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saito T,et al.: "Lipid absorption of the allointestinal grafts under a short course of methyldeoxysupergualin." Transplant Proc. 28. 2570 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mitusada M,et al.: "Prevention of graft rejection and graft-versus-host reaction by novel immunosuppressant, FTY 720, in rat small bowel transplantation." Transplant Int. 10. 343 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Saito T,et al.: "Endoscopic findings in transplanted allo-intestine of rats after discontinuance of immunosuppressive agents." J Gastroenterology (in press). (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Toyama N: "Fulminant second-set allograft rejection and endoscopic findings following small bowel transplantation in the rat." Gastroenterology. 30. 465- (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kiyozaki H: "Segmental small bowel transplantation in the rat:Comparison of lipid absorptioon between jejunal and ileal grafts." JPEN. 20. 67- (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kobayashi E: "Comparison of graft rejection between visualized or newborn intestinal transplantatiton in the rat." Transplant Proc. 28. 2485- (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saito T: "Lipid absorption of the allointestinal grafts under a short course of methyldeoxysupergualin." Transplant Proc. 28. 2570- (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Mitusada M: "Prevention of graft rejection and graft-versus-host reaction by novel immunosuppressant,FTY 720,in rat small bowel transplantation." Transplant Int. 10. 343- (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Saito T: "Endoscopic findings in transplanted allo-intestine of rats after discontinuance of immunosuppressive agents." J Gastroenterology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Toyama N: "Fulminant second-set allograft rejection and endoscopic findings following small bowel transplantation in the rat." Gastroenterology. 30. 465- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kiyozaki E: "Segmental small bowel transplantation in the rat : Comparison of lipid absorption between jejunal and ileal grafts." JPEN. 20. 67- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi E: "Comparison of graft rejection between visualized or newborn intestinal transplantation in the rat." Transplant Proc. 28. 2485- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Saito T: "Lipid absorption of the allointestinal grafts under a short course of methyldeoxysupergualin." Transplant Proc. 28. 2570- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 斎藤 毅: "ラット小腸移植におけるデオキシメチルスパガリン短期投与の効果" 今日の移植. 8. 341- (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] E. Kobayashi, T. Saito et al.: "Comparison of graft rejection between vasculized or new-born intestinal transplantation in the rat." Transplantation Proceeding. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Saoto, E. Kobayashi: "Lipid absorpiton of the allo-intestinal graft under a short course of Methyldeoxyspergualine." Transplantation Proceeding. (in press).

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi