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Deveropment of Cryopreseaved Allograft with Gene Introduction

Research Project

Project/Area Number 07457293
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionOsaka University

Principal Investigator

KADOBA Keisi  Osaka University Medical School, Lecturer, 医学部, 講師 (00185886)

Co-Investigator(Kenkyū-buntansha) IMAGAWA Hiroshi  Osaka University Medical School, Assistant Professor, 医学部, 助手 (90273622)
TAKAHASHI Toshiki  Osaka University Medical School, Assistant Professor, 医学部, 助手 (50263257)
FUKUSHIMA Norihide  Osaka University Medical School, Assistant Professor, 医学部, 助手 (30263247)
SAWA Yoshiki  Osaka University Medical School, Assistant Professor, 医学部, 助手 (00243220)
SHIRAKURA Ryouta  Osaka University Medical School, Professor, 医学部, 教授 (00116047)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1995: ¥5,100,000 (Direct Cost: ¥5,100,000)
KeywordsCryopreseavation / Gene Transfection / 凍結保存法
Research Abstract

Cryopreserved allograft saphenous veins have been one of the alternative conduits used for coronary artery bypass grafting when auotlogous saphenous vein or internal mammary arteries are not available or are inadequate for complete revascularization in Europa and the US.But. its patency is poor. We try gene induction with HVJ liposome for improvement in patency.
Gene introduction with HVJ liposome is good method. We reported the effect that inhibitory gene in cell cycle introduced into vein graft with HVJ liposome.
Jugular vein extirpated from rabbit for cryopreservation in a computer-programd freezer. After storage for more than three weeks, the frozen veins transplanted as interpositional grafts in jugular artery of the same rabbit. In result we established long-term patency cryopreseved graft. Histological analyzes were conducted. At. cryopreserved autograft proliferation of smooth muscle cell was observed beneath the endothelial layr, and smooth muscle layr had thickened. In addition to calcification of the graft was done. Calcification was not found at fresh autograft. This appearance is specific cryopreservation without immunoreaction.
As same as the cryopreserved artery transplanted as interpositional isografts in artery. The endothelium was rejected during the first week after transplantation. Reendothelialization start at two weeks after transplantation. But smooth muscle layr and had not thickened.
An attempt was made to gene introduction at cryopreseved grafts. But patency graft is none. We continued to try.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

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