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Effects of hypoxia on ischemic delayd neuronal death

Research Project

Project/Area Number 07457311
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionFukui Medical University

Principal Investigator

KOBAYASHI Hidenori  Fukui Medical University Neurosurgery Associate Professor, 医学部, 助教授 (80126581)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordscerebral ischaemia / hypoxia / delayd neuronal death / neuronal pentraxin / Mongolian gerbil / hippocampus / 遅発性神経細胞死 / 分子生物学
Research Abstract

1. Gerbils were exposed to 10% oxygen in a chamber for 3 weeks. The bilateral carotid arteries at the neck were occluded for 5 minutes under halothane anaesthesia, recirculated and fed in a normoxia. Animals were sacrificed before and 2,4, and 7 days after surgery. Immunohistochemical study was also done with antibodies against basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). The neuronal death in the hippocampus was severer in the hypoxic group. Expression of both bFGF and VEGF was obvious in the cingulate cortex, corpus callosum and internal capsule before clipping in the hypoxic group, but not observed in the normoxic group before clipping. We observed the expression of both bFGF and VEGF widely in the brain at 2 and 4 days sfter recirculation in both groups. The expression in the hypoxic group was much more prominent than that in the normoxic group. These expressions were not observed at 7 days in both groups.
2. Using a differential display technique, we examined the changes in expression of mRNAs in the hippocampus following 5-minute cerebral ischemia in normoxic Mongolian gerbils. Gerbils were divided into 3 groups (control, 6 hours, 2 days). mRNAs were classified into 3 patterns ; gradual disappearance, decrease and recovery, and new appearance. An mRNA species expressed in the hippocampus in the control group but which decreased at 6 hours after cerebral ischemia and disappeared at 2 days was subcloned and sequenced partly. This mRNA showed homology to neuronal pentraxin. In situ hybridization and Northern blot analyzes showed the same changes in expression as shown by the differential display technique. Neither Western blot nor immunohistochemical analyzes showed differences in neuronal pentraxin protein level among the 3 groups. This observation suggested that neuronal pentraxin plays an important role in delayd neuronal death.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kobayashi H et al.: "Transient cerebral ischemia in mongolian gerbils pre-exposed to hypoxia" ActaNeurochir(Wien). 139 7. 678-683 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi H et al.: "Dynamism of neuronal pentraxin in the gerbil hippocampus following transient cerebral ischemia" J Cereb Blood Flow Metab(Suppl.1). 17. S490- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi H et al.: "Changes in mRNA species in gerbil brain perturbation" Neurol Med Chir(Tokyo)(Suppl.). (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi H,Ishizaki T,Matsukawa S,Kabuto M,Kubota T: "Transient cerebral ischemia in mongolian gerbils pre-exposed to hypoxia." Acta Neurochir (Wien). 139 (7). 678-683 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi H,Matsukawa S,Kabuto M,Handa Y,Kubota T: "Dynamism of neuronal pentraxin in the gerbil hippocampus following transient cerebral ischenia." J Cereb Blood Flow Metab (Suppl.1). 17. S490 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi H,Matsukawa S,Kobayashi S,Kabuto M,Handa Y,Kubota T: "Changes in mRNA species in gerbil brain perturbation." Neurol Med Chir (Tokyo) (Suppl.). (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Ishizaki: "Leukotoxin, 9,10-epoxy-12-octadecenoate causes edematous lung injury via activation of vascular nitric oxide synthase." Am.J.Physiol.269. L65-L70 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Sakai: "Leukotoxin, 9,10-epoxy-12-octadecenoate inhibits mitochondrial respiration of isolated perfused rat lung." Am.J.Physiol. 269. L326-L331 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Ishizaki: "Increased Nitric oxide biosynthesis in Leukotoxin, 9,10-Epoxy-12-Octadecenoate injured lung" B.B.R.C.210. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Ishizaki: "Protective effects of BQ-123, anETA receptor antagonist, against leukotoxin-induced injury in rat lung" Am.J.Physiol. 271. L459-L463 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kobayashi H et al.: "Transient cerebral ischemia in mongolian gerbils pre-exposed to hypoxia." Acta Neurochir(Wien). 139・7. 678-683 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kobayashi H et al.: "Dynamism of neuronal pentraxin in the gerbil hippocampus following transient cerebral ischemia." J Cereb Blood Flow Metab (Suppl.1). 17. S490 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kobayashi H et al.: "Changes in mRNA species in gerbil brain perturbation." Neurol Med Chir (Tokyo) (Suppl.). (in press).

    • Related Report
      1997 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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