Attempts to immortalize vascular smooth muscle cells for the elucidation of the pathogenesis of moyamoya disease
| Project/Area Number |
07457314
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KIKUCHI Haruhiko Kyoto University, School of Medicine, Professor, 医学研究科, 教授 (20072746)
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| Co-Investigator(Kenkyū-buntansha) |
HOSHIMARU Minoru Kyoto University, School of Medicine, Assistant professor, 医学研究科, 講師 (70211539)
NAGATA Izumi Kyoto University, Schoool of Medicine, Assistant professor, 医学研究科, 講師 (10198327)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥5,300,000 (Direct Cost: ¥5,300,000)
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| Keywords | Moyamoya Disease / Superficial Temporal Artery / Immortalization / v-myc / TGF-beta1 / HoxB7 / V-myc / TGFβ1 / 中膜平滑筋細胞 / レトロウィルス |
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| Research Abstract |
Although the primary lesion of moyamoya disease is stenosis of the intracranial arterial trunk, the superficial temporal artery (STA) has been suggested to be involved in moyamoya disease. Immortalization of vascular smooth muscle cells derived from the STA,which was obtained at surgery, was attempted using LINXv-myc retrovirus in order to obtain enough material for molecular biological studies. Although smooth muscle cells infected with the LINXv-myc retrovirus has not been immortalized, they were able to be cultured several times longer than original cells.
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Report
(3 results)
Research Products
(6 results)
