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Bio-Engineering Approach to Mechanisms of Angiogenesis Induced by Blood Flow

Research Project

Project/Area Number 07457326
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

ASATO Hirotaka  UNIVERSITY OF TOKYO,DEPARTMENT OF PLASTIC SURGERY,FACULTY OF MEDICINE,LECTURER, 医学部・附属病院, 講師 (20222581)

Co-Investigator(Kenkyū-buntansha) KAMIYA Akira  UNIVERSITY OF TOKYO,INSTITUTE OF MEDICAL ELECTRONICS,FACLUTY OF MEDICINE,PROFESS, 医学部・附属病院, 教授 (50014072)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥3,600,000 (Direct Cost: ¥3,600,000)
KeywordsMicrocirculation / Angiogenesis / Blood flow / Shear stress / リモデリング / 血行力学的適応 / 血管構築
Research Abstract

The effect of the blood flow and flow-oriented wall shear stress in microvascular angiogenesis was studied in vivo, by employing the rabbit ear chamber technique to quantitatively assess the effect on angiogenesis of chronic administration of the alpha_1 brocker, prazosin, which increases peripheral blood flow. Rabbits were treated with prazosin hydrochloride (50 mg/l in water) orally after ear chambers were installed. The microcirculation in the chamber was observed and recorded from 4 to 23 postoperative day (POD) by an intravital videomicroscope. The relative area covered by vascularized tissue in the chamber (CA), the mean vascular density in the covered area (MD) and the relative vascular area (RA) were quantified. Wall shear stress in venules (20-40mum ID) was also estimated. CA at 7-17 POD,MD at 11-23 POD and RA at 9-23 POD were significantly increased in the prazosin-treated animals. The final RA in the treated group was approximately 21% larger than that in the control group. The measured level of wall shear stress, which was increased 1.8 times by prazosin to at 9 POD,gradually decreased to ward the control level until they met at 13 POD.These findings suggest that the chronichigh flow load enhances microvascular structural change during angiogenesis in vivo, indicating the same adaptive responses of microvessels to wall shearstress as of large vessels. This study has experimentally proven that angiogenesis during tissue repair is also regulated by shear stress due to blood flow.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Ichioka,S.,et al.: "Increased blood flow stimulates remodeling of the microvasculature in the rabbitskin." Biorheology. 33. 84 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka,S.,et al.: "Effects of long-term prazosin treatment on morphologic changes in the microcirculation of the rabbitskin." Microcirculation annual. 11. 179-180 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka,S.,et al.: "Morphometric measurement of changes in the microcirculation of the rabbitskin under long term prazosin treatment." Biorheology. 32. 353 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kosaki,K.,et al.: "In vivo measurement of structural changes in the microcirculation of the skeletal muscle under long term blood flow loading." Biorheology. 33. 84 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kosaki,K.,et al.: "In vivo measurement of structural changes in the microcirculation of the rabbit tenuissimus muscle under long term prazosin treatment." Microcirculation annual. 10. 187-188 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibata,M.,et al.: "Effect of blood flow on angiogenesis." Microcirculation annual. 11. 20-21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka,S.,et al.: "International Journalof Microcirculation,edited by K.Messmer and B.Fragrell" Munich,Germany : Karger, 308 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka, S., K.Kosaki, M.Shibata, and A.Kamiya: "Increased blood flow stimulates remodeling of the microvasculature in the rabbit skin" Biorheology. 33. 84 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka, S., Y.Sato, K.Kosaki, M.Shibata, and A.Kamiya: "Effects of long-term prazosin treatment on morphologic changes in the microcirculation of the rabbit skin" Microcirculation annual. 11. 179-180 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka, S., Y.Sato, K.Kosaki, M.Shibata, and A.Kamiya: "Morphometric measurement of changes in the microcirculation of the rabbit skin under long term prazosin treatment" Biorheology. 32. 353 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kosaki K., S.Ichioka, M.Shibata, and A.Kamiya: "In vivo measurement of structural changes in the microcirculation of the skeletal muscle under long term blood flow loading" Biorheology. 33. 84 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kosaki, K., A.Kamiya, S.Ichioka, and M.Shibata: "In vivo measurement of structural changes in the microcirculation of the rabbit tenuissimus muscle under long term prazosin treatment" Microcirculation annual. 10. 187-188 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibata, M., S.Ichioka, K.Kosaki, and A.Kamiya: "Effect of blood flow on angiogenesis" Microcirculation annual. 11. 20-21 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka, S.et al: International Journal of Microcirculation, edited by K.Messmer and B.Fragrell. Munich, Germany : Karger., 308 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ichioka,S.,et al.: "Increased blood flow stimulates remodeling of the microvasculature in the rabbitskin." Biorheology. 33. 84 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichioka,S.,et al.: "Effects of long-term prazosin treatment on morphologic changes in the microcirculation of the rabbitskin." Microcirculation annual. 11. 179-180 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichioka,S.,et al.: "Morphometric measurement of changes in the microcirculation of the rabbitskin under long term prazosin treatment." Biorheology. 32. 353 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kosaki,K.,et al.: "In vivo measurement of structural changes in the microcirculation of the skeletal muscle under long term blood flow loading." Biorheology. 33. 84 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kosaki,K.,et al.: "In vivo measurement of structural changes in the microcirculation of the rabbit tenuissimus muscle under long term prazosin treatment." Microcirculation annual. 10. 187-188 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shibata,M.,et al.: "Effect of blood flow on angiogenesis." Microcirculation annual. 11. 20-21 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichioka,S.,et al.: "International Journal of Microcirculation,edited by K.Messmer and B.Fragrell." Munich,Germany:Karger, 308 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Ichioka: "Effects of long-term prazosin treatment on morphologic changes in the microcirculation of the rabbit skin" Microcirculation anual. 11. 179-180 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Kosaki: "In vivo measurement of structural changes in the microcirculation of the rabbit tenuissimus muscle under long-term prazosin treatment" Microcirculation anual. 10. 187-188 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Kamiya: "Endothelial cell responses to fluid shear stress. In “Endothelium-derived factors and vascular functions"" Elsevier Science Amsterdam T.Masaki ed.,10 (1994)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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