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THE ROLE OF ADHESION MOLECULES IN THE CHRONICITY AND JOINT DESTRUCTION OF ARTHRITEDES

Research Project

Project/Area Number 07457336
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionKOBE UNIVERSITY

Principal Investigator

ISHIKAWA Hitoshi  Faculty of Health Science, Kobe University School of Medicine, Kobe University, Professor, 医学部, 教授 (30107958)

Co-Investigator(Kenkyū-buntansha) HIRATA Souichiro  Faculty of Health Science, Kobe University School of Medicine, Kobe University,, 医学部, 助教授 (80238360)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥6,600,000 (Direct Cost: ¥6,600,000)
KeywordsRheumatoid arthritis / Joint destruction / Lymphoid infiltrates / Pannus formation / adhesion molecules / Matrix-ligand interaction / Synovial proliferation / 関節炎 / 滑膜炎 / レセプターリガンド反応 / 組織像
Research Abstract

We studied the staining pattern of a group of adhesion molecules in the lining layr and lymphocytic infiltrates of the rheumatoid synovial membrane, using monoclonal antibodies sgainst LFA-1, VLA-4, VLA-5, ELAM-1 and ICAM-1. The cells of the lining layr were strongly ICAM-1 positive and VLA-5 positive, suggesting 1) that ICAM-1 may function to facilitate the adhesion of ICAM-1 bearing type A cells to type B lining cells and 2) that the lining cells may utilize VLA-5 for anchorage to fibronectin at surface of the synovial membrane. In the lymphocyte-rich and transitional areas, the endothelial cells of the venules were both ELAM-1 and ICAM-1 positive. ICAM-1 staining was weak in lymphoid aggregate, but strong in the transitional areas, indcating a paucity of ICAM-1 bearing cells in the lymphocyte-rich areas. On the other hand, LFA-1 staining was very strong in the lymphoid aggregates. This suggested that the large numbers of T4 cells present in the lymphocyte-rich areas are sufficiently activated to express substatial levels of LFA-1, and also that the LFA-1 molecules is an important receptor for emigration from venules. Furthermore to investigate the mechanism of synovial pannus formation in rheumatoid arthritis, immunohistochemical studies were carried out. ICAM-1 positive macrophages and fibroblasts were often found to be contact with lymphoid cells, suggesting that a cellular immune reaction occurs in the formation of the pannus. The VLA-5 molecules was found in a pericellular and interterritorial matrix distribution, strongly suggesting that a receptor-ligand interaction between VLA-5 and cartilage matrix may occur at the early stage of pannus formation. Furthermore, an increase in b1 integrin may be necessary for the growth of the pannus and also for the upregulation of the VLA molecules, leading secondarily to indrease attachment.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Hino, K et al: "EDA-containing fibronectin is synthesized from rheumatoid synovial fibroblast-like cell" Arthritis Rheumatism. 38(5). 678-683 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hirata, S et al: "Inhibition of thrombin-induced cartilage proteoglycan release by argatroban" Bull. Akkied Med. Sci., Kobe. 11. 35-41 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, H et al: "An immunohistochemical and immunoelectron microscopic study of adhesion molecules in synovial pannus formation in rheumatoid arthritis" Bull. Allied Med. Sci., Kobe. 11. 43-52 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, H et al: "An immunohistochemical and immunoelectron microscopic study of adhesion molecules in synovial pannus formation in rheumatoid arthritis" Rheumatology International. 16(2). 53-60 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, H et al: "The role of ICAM-1, LFA-1 and VLA-5 in the lymphocytic infiltrations in rheumatoid synovial membrane" Jap. J. Orthop. Surg. 70(8S). 1596 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ishikawa, H: "Expression of adhesion mlecules in the lymphoid cell distribution in rheumatoid synovial membrane" Bull. Allied Med. Sci, Kobe. 12. 49-60 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 正田悦郎、松崎昭男、平沢泰介、林浩一朗、金田清一、: "膝関節疾患の手術療法" メジカルビュー社, 157 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石川 斉、松井宣夫、: "リウマチのリハビリテーション医学" 医薬ジャーナル社, 271 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] HINO,K ET AL: "EDA-CONTAINING FIBRONECTIN IS SYNTHESIZED FROM RHEUMATOID SYNOVIAL FIBROBLAST-LIKE CELLS" ARTHRITIS RHEUMATISM. 38(5). 678-683 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] KOBU,Y ET AL: "THE CHANGES OF INTRAMUSCULAR OXYGEN TENSION BY LOCAL APPLICATION IN THE RATT" BULL.ALLIED MED.SCIENCES (KOBE). 11. 99-103 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] HIRATA,S ET AL: "INHIBITION OF THROMBIN-INDUCED CARTILAGE PROTEOGLYCAN RELASE BY ARGATROBAN." BULL.ALLIED MED.SCIENCES (KOBE). 11. 35-41 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] ISHIKAWA,H ET AL: "AN IMMUNOHISTOCHEMICAL AND IMMUNOELE CTRON MICROSCOPIC STUDY OF ADHESION MOLECULES IN SYNOVIAL PANNUS FORMATION IN RHEUMATOID ARTHRITIS." BULL.ALLIED MED.SCIENCES (KOBE). 11. 43-52 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] ISHIKAWA,H ET AL: "AN IMMUNOHISTOCHEMICAL AND IMMUNO-ELECTRON MICROSCOPIC STUDY OF ADHESION MOLECULES IN SYNOVIAL PANNUS FORMATION IN RHEUMATOID ARTHRITIS." RHEUMATOLOGY INTERNATIONAL. 16(2). 53-60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] ISHIKAWA,H ET AL: "THE ROLE OF ICAM-1, LFA-1 AND VLA-5 IN THE LYMPHOCYTIC INFILTRATION IN RHEUMATOID SYNOVIAL MEMBRANE." JAP.J.ORTHOP.SURG.70(8S). 1596 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] MIWA,Y ET AL: "CELL DEATH OF THE CHONDROCYTES IN JOINT CARTILAGE" JAP.J.ORTHOP.SURG.70(8S). 1527 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] ISHIKAWA,H.ET AL: "EXPRESSION OF ADHESION MOEICULES IN THE LYMPHOID CELL DISTRIBUTION IN RHEIMATOID SYNOVIAL MEMBRANE" BULL.ALLIED MED.SCIENCES (KOBE). 12. 49-60 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] SHODA,E ET AL: THE TREATMENTOFOSTEO-CHONDRITIS DISSECANS.EDS : MATSUZAKI,A,HIRASAWA,T., HAYASHI,K., AND KANEDA,K "SURGICAL TREATMENT OF KNEE JOINT DISORDERS., 157 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] ISHIKAWA,H: REHABILITATION MEDICINE IN RHEUMATOID PATIENTS.ED.MATSUI,N., 271 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hino,K et al: "EDA-contaning fibronectin is synthesized from rheumatoid synovial fibroblast-like cell" Arthritis Rheumatism. 38(5). 678-683 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirata,S et al: "Inhibition of thronbin-induced cartilage proteoglycan release by argatroban" Bull Allied Medical Sciences,Kobe. 11. 35-41 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa,H et al: "An immunohistochemical and immunoelectron microscopic study of adhesion molecules in synovial pannus formation in rheumatoid arthritis." Bull.Allied Medical Sciences,Kobe. 11. 43-52 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa,H et al: "An immunohistochemical and immunoelectron microscopic study of adhesion molecules in synovial pannus formation in rheumatoid arthritis." Rheumatology International. 16:2. 53-60 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石川斉: "慢性関節リウマチ滑膜組織に浸潤するLymphoid cellにおけるICAM-1,LFA-1,VLA-5の発現について" 日本整形外科雑誌. 70:8. 1596-1596 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa,H et al: "Expression of adhesion molecules in the lymphoid cell distribution in theumatoid synovial membrane" Bull.Allied Med.Sci,Kobe. 12. 49-60 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 正田悦郎: "膝関節疾患の手術療法" 松崎昭男,平沢泰介、林浩一朗、金田清一、メジカルビュー社, 157 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ishikawa, H et al: "The role of adhesion molecules in synovial pannus formation in rheumatoid arthritis." Arthritis Rheum. 37(9). 311 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishikawa, H et al: "B_1-integrin expression in the rheumatoid synovial-pannces formation" Bull Allied Med Sci. 10. 1-9 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hino, K et al: "EDA-containing fibronectin is synthesized from rheumatoid synovial fbroolost-like cell" Arthritis Rheum. 38. 678-683 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] ISHIKAWA, H et al: "An immunohistochemical and immunoclectron microscopic study of adnesion molecules in synovial pannus formation" Rheum Int.(In press). 000-000 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishikawa, H et al: "Adhesion mdecule expression in the lymphoid cell distrilution in rheumatoid synovial membrane." Bull Allied Med Sci. 11(In press). 000-000 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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