| Project/Area Number |
07457340
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KUSUZAKI Katsuyuki Kyoto Prefectural University of Medicine Medicine Lecturer, 医学部, 講師 (30177993)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥5,100,000 (Direct Cost: ¥5,100,000)
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| Keywords | osteosarcoma / multidrug-resistance / overcoming of multidrug-resistance / mouse osteosarcoma cell / adriamycine / DNA ploidy / P-glycoprotein / electoron magmetic field / P糖蛋白質 / 薬剤感受性試験 / アドリアマイシン結合能 |
|---|
| Research Abstract |
Result of DNA ploidy analysis in the study revealed that non-diploid (including aneuploid and euploid-polyploid) osteosarcoma was more sensitive to chemotherapy than diploid one. Adriamycin binding study showed that adriamycin binding ability was closely correlated with histological response to chemotherapy in human osteosarcomas. Multigrug resistant mouse osteosarcoma cells which was established in the study was resistant not only adriamycine also vincristin, bleomycine, mitomycine etc. This cell line was found to be useful for experimental study on overcoming of resistance. In various overcoming methods for resistance, verapamil, cyclosporin A,MS 209, Tween 20 or 80, electoron magnetic field, radiation were useful in mouse osteosarcoma. However, clinically electron magnetic field and MS 209 may available, because two of these were not toxic for human body.
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