| Project/Area Number |
07457345
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kinki University School of Medicine |
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Principal Investigator |
TANAKA Seisuke Kinki Univ.School Med., Dept.Orthop.Surg., Professor, 医学部, 教授 (00026840)
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| Co-Investigator(Kenkyū-buntansha) |
OHTANI Kazuhiro Kinki Univ.School Med., Dept.Orthop.Surg.Assistant, 医学部, 助手 (20258031)
NISHIOKA Shigetoshi Kinki Univ.School Med., Dept.Orthop.Surg.Assistant, 医学部, 助手 (40258033)
FUKUDA Kanji Kinki Univ.School Med., Dept.Orthop.Surg., Lecturer, 医学部, 講師 (50201744)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | Arthrosis / Free radical / Gas mediator / Hyaluronic acid / Interleukin-1 / IL-1 / Chondrocyte / Superoxide / NO |
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| Research Abstract |
Recent observations clearly indicate the significant role of free radical family in the physiological or pathological condition. However, there have been no report indicating the involvement of these molecules in the development of arthrosis. We previously reported that superoxide desmutase inhibited interleukin (IL-1) -mediated cartilage degradation. In this project, we first develop the system to measure the levels of superoxide anion and found that IL-1 induced large amount of superoxide anion from articular cartilage. We also found the involvement of nitric oxide (NO) in IL-1-inhibited proteoglycan. These results are mainly concerning about the involvement of these gas mediators in the development of rheumatoid arthritis. We further demonstrated the increased levels of these molecules in the articular cartilage in experimentally induced osteoarthritis in vivo. Taken together, it is clear that these gas mediators play an crucial role in the disease process of osteoarthrosis and regulation of these molecules could lead to the prevention of disease process of arthrosis. We demonstrated that hyaluronic acid, which is widely used for the treatment of arthrosis clinically, had some regulatory effect on IL-1-induced cartilage degradation.
We believe that these information provide us new strategy for the pharmacological treatment of arthrosis.
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