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Effects of subarachnoid and intravenous administration of lidocaine on glutamate concentration during cerebral ischemia in rats

Research Project

Project/Area Number 07457347
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionAkita University

Principal Investigator

OHTA Sukejuro  Akita Univ., School of Medicine, Assistanat professor, 医学部, 講師 (90125708)

Co-Investigator(Kenkyū-buntansha) MIZUNUMA Takahide  Akita Univ., School of Medicine, Instructor, 医学部, 助手 (50261665)
MASAKI Yoko  Akita Univ., School of Medicine, Instructor, 医学部, 助手 (30125744)
TERADA Hiromichi  Akita Univ., School of Medicine, Instructor, 医学部, 助手 (60282183)
岩崎 洋一  秋田大学, 医学部, 助手 (50282182)
秋山 博美  秋田大学, 医学部, 助手 (40261664)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥3,400,000 (Direct Cost: ¥3,400,000)
KeywordsCerebral ischemia / Glutamate / Local anesthetic / Lidocaine / Microdialysis electrode / Subarachnoid administration / Intravenous administration / マイクロダイアリシス / 大脳皮質 / 海馬 / くも膜下腔 / くも膜下腔投与
Research Abstract

It has been recognized that the accumulation of glutamate during cerebral ischemia may trigger the neuronal injury. We studied whether the pre-ischemic subarachnoid or intravenous administration of lidocaine would influence the rise of extracellular glutamate concentration during transient forebrain ischemia in rats, using the newly developed "dialysis electrode" method.
Exp.1 : Anesthetized rats were administrated lidocaine (5 mg/kg) or normal saline in the cisterna magna. Thereafter 10-min forebrain ischemia was induced by bilateral carotid arteries occlusion and hypotension. We monitored real-time changes of glutamate concentration by the dialysis electrodes implanted in cortex and hippocampal CAl simultaneously before and during ischemia, and reperfusion. As results, during ischemia, glutamate concentrations in these regions showed initial rapid rises, followed by persisting gradual increases during the remainder of ischemia. The maximum values of glutamate concentration in the cort … More ex were lower in the lidocaine group (n=9 ; 107(]SY.+-。[)44muM,mean(]SY.+-。[)SD ; P<0.05) than the saline group (n=9 ; 205(]SY.+-。[)126muM), while those in the hippocampal CAl were similar between groups. With the cession of ischemia, glutamate concentrations rapidly returned to the baselines. Exp.2 : Anesthetized rats were intravenously administrated lidocaine (10 mg/kg) or normal saline. As the first experiment protocol, glutamate concentrations in cortex and hippocampal CAl were measured during 10-min forebrain ischemia. As results, the maximum values of glutamate concentration in each region during ischemia were similar between groups.
The dose of lidocaine intravenously administrated in second study may fail to lead to the effective therapeutic concentration in the cerebral tissue. The accumulation of glutamate in the cortex during transient forebrain ischemia was attenuated by subarachnoid lidocaine. This finding suggests that lidocaine may act as a protective agent against transient cerebral ischemia. Less

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report

Research Products

(8 results)

All Other

All Publications (8 results)

  • [Publications] 水沼 隆秀: "リドカインのくも膜下腔注入による遅発性神経細胞死の程度の軽減効果" 麻酔. 45. 421-427 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 寺田 宏達: "ラット前脳虚モデルの脳内グルタミン酸濃度に及ぼすくも膜下髄液内リドカイン投与の影響" 蘇生. 15. 66- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizunuma T.: "Intravenous and subarachnoid administration of lidocine reduces the delayed neuronal damage in rats." J.Cereb Blood Flow Metab.17. 619- (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizunuma T,Ohta S,Suzuki M: "Subarachnoid administration of lidocaine reduces delayd neuronal damage due to forebrain ischemia in rats." Masui (Jpn J Anesth). 45. 421-7 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Terada H,Ohta S,Mizunuma T,Iwasaki Y.: "Effects of subarachnoid administration of lidocaine on glutamate concentration during forebrain ischemia in rats." Reanimation. 15. 66 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mizunuma T,Ohta S,Nishikawa T: "Intravenous and subarachnoid administration of lidocaine reduces the delayd neuronal damage in rats." J Cereb Blood Flow Metab. 17. S619 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 水沼隆秀: "リドカインのくも膜下腔注入による遅発性神経細胞障害の軽減効果" 麻酔. 45. 421-427 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 水沼隆秀: "リドカインのくも膜下腔注入による遅発性神経細胞障害の軽減効果" 麻酔. 45. (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-03-31   Modified: 2016-04-21  

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