| Project/Area Number |
07457358
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Ehime University (1996)
Kagawa Medical School (1995) |
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Principal Investigator |
SHIRAKAWA Yoichi Ehime University School of Medicine, Dep.of Emergency Medicine, Professor, 医学部, 教授 (90134600)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIYAMA Takashi Kagawa Medical School Hospital, Dep.of Anesthesiology, Assistant Lecturer, 医学部・付属病院, 助手 (60243771)
SEKI K Kagawa Medical School Hospital, Dep.of Emergency Medicine, Assistant Lecturer, 医学部・付属病院, 助手 (20226632)
AMENO Kiyoshi Kagawa Medical School, Dep.of Forensic Medicine, Assistant Professor, 医学部, 助教授 (50019626)
TSUKAMOTO Ikuko Kagawa Medical School, Dep.of Anesthesiology & Emergency Medicine, Assistant Lec, 医学部, 助手 (10183477)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | FT-IR spectroscopy / expired gas analysis / chemical intoxication / volatile poison / FT IR分光分析 / FT-IR / 長光路気体セル |
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| Research Abstract |
We applied FT-IR spectroscopy (Perkin Elmer type1600) with a long path cell (Infrared Analysis Inc.ZnSe-windows) to the determination of trace volatile substances in expired gas. Good liner correlations between concentration and absorbance were shown for ethanol (1.6-1600 mug/L) using 2988 cm^<-1> absorption, and for toluen (87-8700 mug/L) using 3043 cm^<-1> absorption. Serial analysis of ethanol in expired gas (with FT-IR) and in blood (with headspace-gaschromatography) after the ethanol ingestion (0.5g/kg) in 20 normal volunteers were performed. Ratio of ethanol concentration in blood and expired gas was low during the initial phase, then increased and approached asymptotes (approximately 3,000) three hours after the injestion, which suggests that the measured values of ethanol concentration in expired gas might be overestimated, possibly because of evaporated ethanol in the esophagus and the stomach. This hypothesis was supported by the experiment that tracheostomy kept the blood/gas ratio constant (approximately 3,000) for six hours after the ethanol ingestion in rabbits. The rabbit experiments also revealed that simultaneous determination of ethanol and toluen in exoired gas was possible. FT-IR spectroscopy with a long path cell was suggested to be a valuable tool not only for the screening of volatile poisons but also for the kinetic study of them.
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