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Basic research in pathogenesis of BPH and phermaceutical therapy for BPH

Research Project

Project/Area Number 07457367
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionGunma University

Principal Investigator

YAMANAKA Hidetoshi  Gunma university, School of medicine Pofessor, 医学部, 教授 (70110393)

Co-Investigator(Kenkyū-buntansha) FUKABORI Yoshitatsu  Gunma university, School of medicine Assistant, 医学部, 助手 (90199167)
YUASA Hisako  Gunma university, School of medicine Assistant, 医学部, 助手 (50240148)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBPH / estrogen receptor / rat seminal vesicle / stromal cell / androgen receptor / pathogenesis / ラット精嚢 / 間質 / ER / 前立腺肥大症 / エストロジェンレセプター
Research Abstract

On the basis of the working hypothesis that the essential pathogenesis of human benign prostatic hyperplasia might be the stromal proliferation, we are going to investigate how sex hormones and growth factors are involved in the stromal proliferation. We have already established the experimental model for stromal proliferation of male reproductive organs, that is, the stromal proliferation in the seminal vesicle after E2 injection to the immature castrated rats. We investigated the participation of estrogen in the stromal proliferation using this rat seminal vesicle model. On the other hand, in order to obtain the further understanding of the mechanism of stromal proliferation in human benign prostatic hyperplasia, we did the in vitro experiment using the cultured prostate stromal cells. We obtained the following results in 1996.
1) The experimental model : In 1995, we observed that the increase of ER (estrogen receptor) protein in the stromal cells concurred with the stromal proliferation. In 1996, we found that ER protein appeared in the basal cell as well as the stromal cell. Moreover, we found that the concomitant treatment of E2 with 5 alpha-DHT completely inhibited E2 mediated ER expression in both basal cell and stromal cell. This result suggests that ER works only when E2 is given in the absence of 5alpha-DHT.
2) The cultured prostate stromal cell : In 1995, Wwe observed that TGF-beta secretion from the cultured stromal cell increased in proportion to the decrease of DHT concentration in the culture-medium. In 1996, TGF-beta upregulated collagen production from the cultured stromal cell in the autocline fashion. Moreover, we found that this stromal cell had AR (androgen receptor) and expressed KGF in the presence of 5alpha-DHT.This result suggests that there exists DHT-AR-KGF system in this stromal cell.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Yuasa,H.: "Effect of sex hormones on the tissue localization of nuclear estrogen receptor positivery stained cells in the seminal vesicle of immature castrated rats." Tohoku J.Exp.Med.181(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 湯浅久子: "幼若去勢ラット精嚢におけるエストロジュン効果発現時のエストロジュンレセプターの変動" Acta Urol.Japon.42. 33-37 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,K.: "Effect of aromatase inhibitor,TZP-2209,on the prostate of androstenedione-treated castrated dogs : changes in prostate volume and histopathological findings." Prostate. 28. 328-337 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukabori,Y.: "Androgen and prostatic stromal cells." Proceedings of the seventh workshop on prostate cancer. 11-14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukabori,Y.: "Androgen reduction stimulates collagen production of human prostate smooth muscle cell." Proceedings of the american urological association. 153 300A(Suppl.). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ono,Y.: "Eosinophiles infiltration in the rat seminal vesicle associated with estradiol-17 β-related stromal proliferation." Tohoku J.Exp.Med.175. 163-169 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yuasa, H.: "Effect of sex hormones on the tissue localization of nuclear estrogen receptor positivery stained cells in the seminal vesicle of immature castrated rats." Tohoku J.Exp.Med.181 (in press). 00-00 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yuasa, H.: "The estrogen-induced changes of estrogen receptor in seminal vesicle of immature castrated rat." Acta Urol.Japan.42. 33-37 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, K.: "Effect of aromatase inhibitor, TZP-2209, on the prostate of androstenedione-treated castrated dogs : changes in prostate volume and histopathological findings." Prostate. 28. 328-337 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukabori, Y.: "Androgen and prostatic stromal cells." Proceedings of the seventh workshop on prostate cancer. 11-14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukabori, Y.: "Androgen reduction stimulates collagen production of human prostate smooth muscle cell." Proceedings of the american urological association. 153 300A suppl. (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ono, Y.: "Eosinophiles infiltration in the rat seminal vesicle associated with estradiol-17 beta-related stromal proliferation." Tohoku J.Exp.Med.175. 163-169 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yuasa,H.: "Effect of sex hormones on the tissue localization of nuclear estrogen receptor positively stained cells in the seminal vesicle of immature castrated rats." Tohoku J. Exp. Med.181. 00-00 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 湯浅久子: "幼若去勢ラット精嚢におけるエストロジェン効果発現時のエストロジェンレセプターの変動" 泌尿紀要. 42. 33-37 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ono,Y.: "Eosinophiles infiltration in the rat seminal vesicle Associated with estradiol-17β-related stromal proliferation" Tohoku J. Exp. Med.175. 163-169 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,K.: "Synergistic effects of estrogen with androgen on the prostate-effects of estrogen on the prostate of androgen-administrated rats and 5-alpha-redactase activity" Prostate. 25. 169-176 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukabori,Y.: "Rapid induction of keratinocyte growth factor (FGF-7) and β-actin after exposure of prostate stromal cells to androgen" In Vitro Cell Dev. Biol.30A. 745-746 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] 湯浅久子: "幼若去勢ラット精嚢におけるエストロジェン効果発現時のエストロジェンレセプターの変動" 泌尿紀要. 42. 33-37 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ono, Y.: "Eosinophiles infiltration in the rat seminal vesicle Associated with estradiol-17β-related stromal proliferation" Tohoku J. Exp. Med.175. 163-169 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki, K.: "Synergistic effects of estrogen with androgen on the prostate-effects of estrogen on the prostate of androgen-administrated rats and 5-alpha-redactase activity" prostate. 25. 169-176 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukabori, Y.: "Rapid induction of keratinocyte growth factor (FGF-7) and β-actin after exposure of prostate stromal cells to androgen" In Vitro Cell Dev. Biol.30A. 745-746 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 湯浅久子: "ラット雄性副性器の腺管上皮および間質のホルモン反応特性" 泌尿紀要. 39. 631-637 (1993)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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