| Project/Area Number |
07457374
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
NAITO Katsusuke Yamaguchi University School of Medicine, Professor, 医学部, 教授 (60115251)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIHIRO Satoru Yamaguchi University School of Medicine, Assistant, 医学部, 助手 (40284260)
YAMAMOTO Mitsutaka Yamaguchi University School of Medicine Hospital, Assistant Professor, 医学部・附属病院, 講師 (80243640)
島袋 智之 山口大学, 医学部・附属病院, 助手 (60226222)
松山 豪泰 山口大学, 医学部・附属病院, 講師 (70209667)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | rG-CSF / Urological malignant tumor cells / Proliferation / in vitro / G-CSF |
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| Research Abstract |
The cultivation of PBMCs with OK-432 inhibited the production of rG-CSF by PBMCs. The inhibition may play a role in the mechanism of the cytokine-mediated antitumor effect of OK-432.
Tumor cells such as KK-47 cells and T24 cells, and PBMCs were seeded in separated agar layr each other in the following experiments. Any cytokines such as IL-6, EGF,basic-FGF,IL-2 were not detected in the supernatant of cultured PBMCs. KK-47 cells constitutively produced basic-FGF and IL-6 in the supernatant and T24 cells produced IL-6. When KK-47 cells were cultured with rG-CSF for 96 hours, productio of basic-FGF by KK-47 cells increased in a dose-dependent manner until rG-CSF concentraion of 10 ng/ml. Furthermore, the production of basic-FGF increased under the presence of both of PBMCs and rG-CSF in the culture medium. When basic-FGF was added into culture medium, cell number estimated by MTT assay of KK-47 cells increased in a dose-dependent manner on basic-FGF concentrations. Cell number of KK-47 cells cultured with both of rG-CSF and PBMCs significantly increased than those cultured with rG-CSF only. Cell number of S-phase fraction estimated by FCM of KK-47 cells increased in cultivation with rG-CSF,however, that of T24 cells did not in the same condition. In RT-PCR,KK-47 cells expressed mRNAs of IL-6 receptor, FGF receptor 1 and rG-CSF receptor, however, no expression of mRNA of FGF receptor 2 was observed. T24 cells expressed mRNA of IL-6, however, no expression of mRNA of rG-CSF was observed. PBMCs expressed mRNAs of rG-CSF,FGF receptor 1 and 2. It was suggested that IL-6 and jbasic FGF might be autocrine factors of KK-47 cells. Production of basic FGF by KK-47 cells activated by rG-CSF might take part in effect of rG-CSF on proliferation of KK-47 cells.
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