| Project/Area Number |
07457384
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
KURAUCHI Osamu Nagoya University, Faculty of Medicine, Lecturer, 医学部, 講師 (80195528)
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| Co-Investigator(Kenkyū-buntansha) |
MIZUTANI Shigehiko Nagoya University, Faculty of Medicine, Professor, 医学部, 教授 (00159162)
MURATA Yishiharu Nagoya University, Research Institute of Environmental Medicine, Professor, 環境医学研究所, 教授 (80174308)
ITAKURA Atsuo Nagoya University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (70262897)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Preeclampsia / Specific gene / Placenta / hepatocyte growth factor / Differential PCR |
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| Research Abstract |
The purpose of this study was to elucidate the possible relationship between hepatocyte growth factor (HGF) expression and the pathogenesis of preeclampsia. The concentration of immunoreactive HGF was measured and the expression og HGF messenger ribonucleic acid (mRNA) assessed in human placentas obtained from two groups ; uncomplicated and preeclamptic pregnancies at various gestational weeks. In addition, the localization of HGF mRNA and c-met protein was analyzed using in situ hybridization and immunohistochemical staining, respectively. The expression of HGF mRNA and the concentration of immunoreactive HGF were highest in the second trimester, and were significantly decreased in preeclamptic placentas compared to the uncomplicated cases in the third trimester. HGF mRNA was localized to placental mesenchymal cells, whereas c-met protein was demonstrated on cytotrophoblast. These results provide evidence of an abnormality of HGF expression in the preeclamptic placentas. Such placentas exhibit the abnormally shallow trophoblast invasion of the uterus and reduced expression of HGF could well account for this morphometric change.
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