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Title of Project ; Analysis of differentiation of human luteal cells, using monoclonal antibodies that recognize differentiation-related antigens on luteal cells.

Research Project

Project/Area Number 07457385
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MORI Takahide  Dept.of Gynecology and Obstetrics, Faculty of Medicine, Professor, 医学研究科, 教授 (90026865)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Hiroshi  Dept.of Gyn/Ob, Faculty of Medicine, Associate Professor, 医学研究科, 講師 (30252456)
今井 公俊  京都大学, 医学研究科, 助手 (90252436)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1995: ¥3,800,000 (Direct Cost: ¥3,800,000)
Keywordsmonoclonal antibody / corppus luteum / luteal cells / differenttiation / gonadotropin / cytokine / integrin / extracellular matrix / 分化抗原 / 抗原精製 / アミノ酸シークエンス
Research Abstract

Summary of Research Results
Corpus luteum (CL) has important physiological functions in the female reproduction. It is generally accepted that corpus luteum is essential for successful pregnancy. Luteal dysfunction is assumed to be one of causes of infertility and/or habitual abortion.In addition, it is suggested that luteal insufficiency may cause endometrial hyperplasia and cancer. Furthermore, during the luteal phase, luteal cells show morphological and functional changes. During the CL formation, follicular cells differentiate to luteal cells, and steroidogenic enzymes are induced to produce progesterone and so on. During the CL regression, luteal cells demonstrate apoptotic changes. Therefore, luteal cells are useful materials for analysis of cell differentiation. However, differentiation of luteal cells are not understood, to date.
To investigate the differentiation pathways and regulatory mechanisms of differentiation of human luteal cells in the ovary, we raised murine monoclonal … More antibodies (mAbs) against human luteal cells, and analyzed the cell-lineage-and differentiation stage-specifically expressed molecules on cell surface. (1) We have obtained several mAbs that recognize granulosa cell lineage-specific or thecal cell lineage-specific cell surface molecules. From these data, we proposed the human granulosa cells differentiate to large luteal cells of the menstrual CL and also CL of early pregnancy, and that human theca interna cells differentiate to small luteal cells. (2) We have found expression profiles of several molecules are different between the menstrual CL and CL of pregnancy. This suggest that CL of pregnancy have different functions from CL of the cycle. (3) Though the study on the effect of gonadotropins and cytokines on the expression of the differentiation-related antigens on luteal cells, we have found that differentiation of luteal cells are regulated not only by gonadotropins but also by cytokines. This suggests that immunological factors are involved in the differentiation of menstrual CL to CL of pregnancy. (4) We have found that the interaction between integrins (cell adhesion molecules) and extracellular matrices are local regulator of the differentiation of luteal cells. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Fujiwara H et al.: "A porcine homolog of human integrin a6 is a differentiation antigen of granulosa cells." Biology of Reproduction. 53. 407-417 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda T et al.: "Integrin a6 is a differentiation antigen of human granulosa cell." Journal of Clinical Endocrinol and Metabolism. 80. 2899-2905 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiwara H et al.: "Granulosa cells express integrin a6 : possible involvement of integrin a6 on folliculogenesis." Hormone Research. 46 (suppl 1). 24-30 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] achibana T et al.: "An aminopeptidase inhibitor, bestatin, enhances FSH-stimulated progesterone and oestradiol secretion of porcine granulosa cells in vitro." Human Reproduction. 11. 497-502 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiwara H et al.: "A differentiation antigen of human large luteal cells in corpora lutea of the menstrual cycle and early pregnancy." Biology of Reproduction. 54. 1173-1183 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura K et al.: "An aminopeptidase inhibitor, bestatin, enhances gonadotropin-stimulated ovulation in mice." Human Reproduction. 11. 1952-1957 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takabatake K et al.: "Human Reproduction" Intravenous administration of splenocytes in early pregnancy changed implantation window in mice. (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiwara H,et al.: "A porcine homolog of human integrin alpha6 is a differentiation antigen of granulosa cells" Biol Reprod.53. 407-417 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honda T,et al.: "Integrin alpha6 is a differentiation antigen of human granulosa cell" J Clin Endocrinol Metab.80. 2899-2905 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiwara H,et al.: "Granulosa cells express integrin alpha6 : possible involvement of integrin alpha6 on folliculogenesis." Horm.Res.46 (suppl 1). 24-30 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tachibana T,et al.: "An aminopeptidas inhibitor, bestatin, enhances FSH-stimulated progesterone and oestradiol secretion of porcine granulosa cells in vitro" Hum.Reprod. 11. 497-502 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiwara H,et al.: "A differentiation antigen of human large luteal cells in corpora lutea of the menstrual cycle and early pregnancy." Biool.Reprod.54. 1173-1183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura K,et al.: "An aminopeptidase inhibitor, bestatin, enhances gonadotropin-stimulated ovulation in mice" Hum.Reprod.11. 1952-1957 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takabatake K,et al.: "Intravenous administration of splenocytes in early pregnancy changed implantation window in mice" Hum.Reprod.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiwara H et al.: "A porcine homolog of human integrin a6 is a differentiation antigen of granulosa cells." Biology of Reproduction. 53. 407-417 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Honda T et al.: "Integrin a6 is a differentiation antigen of human granulosa cell." Jouranl of Clinical Endoocrinol and Metabolism. 80. 2899-2905 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hujiwara H et al.: "Granulosa cells express integrin a6 : possible involvement of integrin a6 on folliculogenesis." Hormone Research. 46(suppl1). 24-30 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tachibana T et al.: "An aminopeptidase inhibitor,bastatin,enhances FSH-stimulated progesterone and oestradiol secretion of prcine granulosa cells in vitro." Human Reproduction. 11. 497-502 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujiwara H et al.: "A differentiation antigen of human large luteal cells in corpora lutea of the menstrual cycle and early pregnancy." Biology of Reproduction. 54. 1173-1183 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakamura K et al.: "An aminopeptidase inhibitor,bastatin,enhances gonadotropin-stimulated ovulatioon in mice." Human Reproduction. 11. 1952-1957 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takabatake K et al.: "Human Reproduction" Intravenous administration of splenocytes in early pregnancy changed implantatioon window in mice.(in press), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujiwara H, et al.: "A differentiation antigen of human large luteal cells in corpora lutea of the menstrual cycle and early pregnancy." Biology of Reproduction. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujiwara H, et al.: "Granulosa cells express integrin α6: possible involvement of integrin α6 on folliculogenesis." Hormone Research. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Honda T, et al.: "Integrin α6 is a differentiation antigen of human granulosa cell." Journal of Clinical Endocrinology & Metabolism. 2899-2905 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Imai K, et al.: "A mouse monoclonal antibody, S2n8, detects a 142kD protein on the surface of human endometrial stromal cells and decidual cells." Human Reproduction. 10. 1311-1318 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujiwara H, et al.: "A porcine homolog of human integrin α6 is a differentiation antigen o f granulosa cells." Biology of Reproduction. 53. 407-417 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hattori N, et al.: "Human luteal cells express leukocyte functional antigen(LFA)-3." Journal of Clinical Endocrinology & Metabolism. 80. 78-84 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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