| Project/Area Number |
07457392
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Yokohama City University |
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Principal Investigator |
TAGA Michiyoshi Yokohama City University, School of Medicine, Assist.Prof., 医学部, 助教授 (00107682)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGETA Hiroyuki Yokohama City Univ.OB/GYN,Associa.Prof., 医学部, 講師 (20275051)
TAKAHASHI Tsuneo Yokohama City Univ.Associa.Prof., 医学部, 講師 (60179497)
木田 博勝 高知医科大学, 医学部, 助手 (50264627)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1997: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | KGF / endometrium / decidua / KGF receptor / gene expression / trophoblast / hCG / menstrual cycle / KGFmRNA / hCG / 細胞増殖 / 繊毛 / RT-PCR / KGF受容体mRNA / Northern blot / 増殖 / 分化 / 成長因子 |
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| Research Abstract |
In order to clarify a role of keratinocyte growth factor (KGF) in the regulation of human endometrial function and implantation, we investigated the changes in KGF mRNA in endometrium during menstrual cycle and in decidua of early pregnancy as well as the biological effect of KGF on trophsblastic cells.
The levels of KGF mRNA increased in luteal phase and in early pregnancy, suggesting that it is under the control of progesterone. We also demonstrated the HGF receptor mRNA in chorionic villi and choriocarcinoma cells. Thus, KGF produced in decidual cells acts on trophoblasts in a paracrine fashion, KGF enhanced hCG secretion from choriocarcinoma cell line in vitro, but not influence cell proliferation.
These results indicate that KGF plays an important role in endometrial/decidual-trophoblastic interaction.
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