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FOS dependent transcriptional regulation in the retina

Research Project

Project/Area Number 07457408
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

MATSUDA Hidehiko  Department of Ophthalmology, Hokkaido University School of Medicine, Professor, 医学部, 教授 (80000959)

Co-Investigator(Kenkyū-buntansha) IMAKI Junko  Department of Anatomy, Nippon Medical School, Associate Profgessor, 医学部, 講師 (20223323)
YOSHIDA Kazuhiko  Department of Ophthalmology, Hokkaido University School of Medicine, Instructor, 医学部, 助手 (90281807)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1995: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsC-fos / CREB / Retina / Light / Injury / calcium / calmodulin / transcription / fos / Reting / Dark / 網膜 / 光 / 外傷 / カルシウム / 分子生物学 / c-fos遺伝子 / cAMP / 転写制御 / 明暗変化
Research Abstract

The signal pathway of light-induced expression of the neuropeptide somat ostain (SS) in retinal cells was investigated. Flashing light induced c-fos and SS in the inner nuclear layr (INL) and the ganglion cell layr (GCL). As both c-fos and SS genes have a cAMP responsive element (CRE) in their promaters, CREB phosphorylation in retinal cells was examined with phospho-CREB specific antibody. Both flashing light and administration of the L-type Ca^<2+> channel activator Bay K 8644 induced phosphorylation of CREB in the nuclei of the same portion of the retina where c-fos/SS mRNAs were expressed. The distribution of calmodulin kinase II immunoreactive retinal cells was also in good accordance with the phospho-CREB immunoreactivity. Thus we propose that the transcriptional response to light stimulation is regulated via CREB phosphorylation catalysed by Ca^<2+>/calmodulin-dependent protein kinase in the neural retina.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yoshida K, et al.: "Light-induced creb phosphorylation andgene expression in rat retinal" Journal of Neurochemistry. 65・4. 1449-1504 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yosida K, et al.: "Differntial distribution of CaM Kinases and induction of C-fos expression by flashing Light in cat retinal cells" Inv.Ophthalmol,Vis,Sci.37・1. 174-179 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada T.et al.: "Cone-associated C-fos gene expression in the light-damaged rat" Inv.Ophthalmol,Vis,Sci.37・7. 1250-1255 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada T.et al.: "Phosphorylation of creb in rat retinalcells after focal retinal injury" Experimental Eye Research. 61・6. 769-771 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohki, K, et al.: "Jun-b gene experssion in rat retinal cells following focal retinal injury" Current Eye Research. 14・11. 1021-1024 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida K, et al.: "Differntial expression of maf-1 and maf-2 genes in the developing rat lens" Investigative Ophthalmology & visual Scinece. 38・12. 2679-2683 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada, T,Imaki, J,Hagiwara, M,Ohki, K,Takamura, M,Ohashi, T,Matsuda, H,and Yoshida, K.: "Phosphorylation of creb in rat retinal cells after focal retinal injury." Experimental Eye Research. 61(6). 769-771 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harada, T,Imaki, J,Ohki, K,Ono, K,Ohashi, T,Matsuda, H,and Yoshida, k.: "Cone-associated c-fos gene expression in the light-damaged rat retina." Investigative Ophthalmology & Visual Science. 37(7). 1250-1255 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohki, k, Yoshida, K,Yamakawa, A,Harada, T,Matsuda, H,and Imaki, J.: "Jun-b gene expression in rat retinal cells following focal retinal injury." Current Eye Research. 14(11). 1021-1024 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K,Imaki, J,Fujisawa, H,Harada, T,Ohki, K,Matsuda, H,and Hagiwara, M.: "Differential distribution of CaM kinases and induction of c-fos expression by flashing and sustained light in rat retinal cells." Inv.Ophthalmol.Vis.Sci.37(1). 174-179 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K,Imaki, J,Koyama, Y,Harada, T,Shinmei, Y,Oishi, C,Matsushimahibiya, Y,Matsuda, A,Nishi, S,Matsuda, H,and Sakai, M.: "Differential expression of maf-1 and maf-2 genes in the developing ratlens." Investigative Ophthalmology & Visual Science. 38(12). 2679-2683 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K,Imaki, J,Matsuda, H,and Hagiwara, M.: "Light-induced creb phosphorylation and gene expression in rat retinal cells." Journal of Neurochemistry. 65(4). 1499-1504 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida K, Imaki J, et al.,: "Differential expression of maf-1 and maf-2 genes in the developing rat lens." Inv.Ophthalmol.Vis.Sci.38. 2679-2683 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Imaki J, Yoshida K, et al.,: "Presence of ERK2 in rat retinal cells." Current Eye Reserch.16. 957-959 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sakai M, Imaki J, et al.,: "Rat mat Related genes:Specific Expression in Chondrocytes,Lens and spinal Cord." Oncogene. 14. 745-750 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida et al.: "Light-Indvced CREB Prosphorylation and Gene Expression in Rat Retinal Cells" Jaurnal of Neurochemistry. 65. 1499-1509 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida et al.: "C-fos Gene Expicssion in Rat Retinaltells After Focal Retinal Injury" Investigative Ophthalmology & Visual Science. 36. 251-254 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harada et al.: "Phosphorylation of CREB in Rat Retinal Cells after Focal Retinal Injury" Exp. Eye Res.61. 769-772 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harada et al.: "Cone-Acsociated c-fos Gene Exprcssion in the Ligth-Damaged Rat Retina" Investigative Ophthalmology & Visual Science. 37. 1250-1255 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida et al.: "Differential Distribution of CaM Kinases and Induction of C-fos Expression by Flashing and Sustained Light in Rat Rernal Cell" Investigative Ophthalmology & Visual Science. 37. 174-179 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida et al.: "Light-induced creb phosphorylation and gene expression in rat retinal cells." J.Neurchem.65. 1499-1509 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohki et al.: "Nitric oxide induces c-fos gene expression via cyclic amp response element binding protein(creb) phosphorylation in rat retinal pigment epithelium." Brain Res.696. 140-144 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshida et al.: "Differential Distribution of CaM Kinases and Induction of c-fos Expression by Flashing and Sustained Light in Rat Retinal Cells." Inv.Ophtshalnol.Vis.Sci. 37. 174-179 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Harada et al.: "Phosphorylation of CREB in rat retinal cells after foca retinal injury." Exp.Eye Res.61. 769-772 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohki et al.: "c-fos gene expression in postnatal rat retinas with light/dark cycle." Vision Res.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Harada et al.: "Cone-associated c-fos Gene Expression in the Light Damaged Rat Retina." Inv. Ophthalmol,Vis.Sci. (in press).

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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