| Project/Area Number |
07457408
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
MATSUDA Hidehiko Department of Ophthalmology, Hokkaido University School of Medicine, Professor, 医学部, 教授 (80000959)
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| Co-Investigator(Kenkyū-buntansha) |
IMAKI Junko Department of Anatomy, Nippon Medical School, Associate Profgessor, 医学部, 講師 (20223323)
YOSHIDA Kazuhiko Department of Ophthalmology, Hokkaido University School of Medicine, Instructor, 医学部, 助手 (90281807)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1995: ¥4,300,000 (Direct Cost: ¥4,300,000)
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| Keywords | C-fos / CREB / Retina / Light / Injury / calcium / calmodulin / transcription / fos / Reting / Dark / 網膜 / 光 / 外傷 / カルシウム / 分子生物学 / c-fos遺伝子 / cAMP / 転写制御 / 明暗変化 |
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| Research Abstract |
The signal pathway of light-induced expression of the neuropeptide somat ostain (SS) in retinal cells was investigated. Flashing light induced c-fos and SS in the inner nuclear layr (INL) and the ganglion cell layr (GCL). As both c-fos and SS genes have a cAMP responsive element (CRE) in their promaters, CREB phosphorylation in retinal cells was examined with phospho-CREB specific antibody. Both flashing light and administration of the L-type Ca^<2+> channel activator Bay K 8644 induced phosphorylation of CREB in the nuclei of the same portion of the retina where c-fos/SS mRNAs were expressed. The distribution of calmodulin kinase II immunoreactive retinal cells was also in good accordance with the phospho-CREB immunoreactivity. Thus we propose that the transcriptional response to light stimulation is regulated via CREB phosphorylation catalysed by Ca^<2+>/calmodulin-dependent protein kinase in the neural retina.
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