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INVESTIGATION ABOUT THE MECHANISM OF RETINAL ADHESION AND ITS BREAKDOWN AND RECOVERY

Research Project

Project/Area Number 07457413
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionKUMAMOTO UNIVERSITY

Principal Investigator

NEGI Akira  KUMAMOTO UNIVERSITY,OPHTHALMOLOGY,PROFESSOR, 医学部, 教授 (00189359)

Co-Investigator(Kenkyū-buntansha) HIRATA Akira  KUMAMOTO UNIVERSITY,OPHTHALMOLOGY,ASSISTANT PROFESSOR, 医学部附属病院, 助手 (60295144)
MURATA Yasuhiro  KUMAMOTO UNIVERSITY,OPHTHALMOLOGY,ASSISTANT PROFESSOR, 医学部, 助手 (80229997)
MIYAGAWA Shin-ichi  KUMAMOTO UNIVERSITY,OPHTHALMOLOGY,ASSISTANT PROFESSOR, 医学部, 助手 (10260738)
MATSUMOTO Koki  KUMAMOTO UNIVERSITY,OPHTHALMOLOGY,ASSOCIATE PROFESSOR, 医学部, 助教授 (70173896)
小島 祐二郎  熊本大学, 医学部付属病院, 助手 (10178270)
稲田 晃一朗  熊本大学, 医学部付属病院, 講師 (60184719)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1997: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsRETINA / RETINAL PIGMENT EPITHELIUM / RETINAL DETACHMENT / RETINAL BREAK / GAS TANPONADE / VITRECTOMY / SUBRETINAL FLUID / ガスタンポナ-デ / 黄班円孔 / 硝子体 / 中心性網膜炎 / 網膜円孔
Research Abstract

1 Healing process of the rabbit retinal pigment epithelium after mechanical removal A dome shaped retinal detachment was created in the rabbit eye by injecting saline into the subretinal space. RPE under the detached retina was removed from Bruck's membrane about 1.5mm in diameter and the healing process was observed morphologically. Shortly after the injury, RPE cells around the wound started to fill the defected area by sliding and migrating, and after 2 weeks the wound was completely covered. In eyes with overlying sensory retina, regenerated RPE cells formed a monolayr similar to normal RPE.The microvilli and RPE-photoreceptor outer segment interface was reorganized. In eyes with no sensory retina, the regenerated RPE were multilayred and the microvilli were short and disorganized. In many cases of damaged Bruck's membrane, choroidal neovascularization was observed.
2 Sealing process of the retinal hole in the rabbit eye
Retinal hole of 0.5mm in diameter was made in the rabbit eye. After making artificial posterior vitreous detachment, vitrectomy and gas tamponade were performed. One week after surgery, the retinal hole was perfectly closed in all eyes macroscopically.
Histologically, the hole was filled with glial cells, fibroblast and RPE.Diaminido yellow staining revealed that glial cells accumulated actively to the retinal wound and this process playd important roll to seal the hole.
3 Effects of steroid on the subretinal fluid resorption
Experimental serous detachment was made by injecting rose bengal and mild photocoagulation in the rabbit eye. Preliminary results showed the tendency that in a group of administrating steroid, the serous detachment survived longer than that in non-administered group.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 根木 昭: "接着機構から見た網膜疾患" 第一回国際眼科シンポジウム. 120-121 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] AKIRA NEGI: "RETINAL DISEASES INVESTIGATION FROM THE STANDPOINT OF ADHESION MECHANISM" THE FIRST INTERNATIONAL SYMPOSIUM OF OPHTHALMOLOGY,JAPAN OPHTHALMIC SOCIETY. 120-121 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary

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Published: 1995-04-01   Modified: 2016-04-21  

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