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Ditribution of bone matrix proteins and PTH recepter mRNA during ectopic bone formation induced by BMP

Research Project

Project/Area Number 07457428
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionOkayama University

Principal Investigator

NAGATSUKA Hitoshi  Okayama University Dental School Associate Professor, 歯学部, 助教授 (70237535)

Co-Investigator(Kenkyū-buntansha) AKAGI Takumi  Okayama University Dental School Research Assistant, 歯学部, 教務員 (50192878)
MURATA Masaru  Okayama University Dental School Assistant, 歯学部, 助手 (00260662)
INOUE Masahisa  Okayama University Dental School Assistant, 歯学部, 助手 (20223274)
NAGAI Noriyuki  Okayama University Dental School Professor, 歯学部, 教授 (90085770)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsBMP / mRNA / in situ hybridization / bone matrix protein / collagen / non RI / PTH prcepter
Research Abstract

Purtialy purified bovine BMP-collagen I : 1 W after implantation. Osteopontin mRNA expression was found mainly at the central area of the tissue. At higher magnification, strong osteopontin mRNA expression was seen in large ovary shaped cells Osteonectin mRNA expression was found in the central to the middle area of the tissue. Osteonectin mRNA expression seen in fibroblast-like cells. Osteocalcin mRNA expression could not be detected at 1W after implantatin. Type I collagen mRNA expresison was found in all area. 2 W after implantation. Strong osteopontin and osteonectin mRNA expression was found in almost all areas of the tissue. At higher magnificaiton, strong osteonectin mRNA expression was seen in chondrocytes, osteoblasts, and young osteocytes. Osteocalcin mRNA expression was found in osteoblasts and osteoxytes of the newly formed bone. Type II and X collagen mRNA expression was found in chondrocytes and few osteoblast. 3 W after implantation. Osteopontin mRNA expression was found … More in osteoblasts surrounding the trabecular-shaped woven bone. Strong osteonectin mRNA expression was found in osteoblasts an osteocytes. Osteocalcin mRNA expression was found in osteoblasts and young osteocytes. Type I collagen mRNA espression was found in osteoblasts and young osteocytes.
rhBMP-2-IMB : In the heterotopic cartilage like tissue, Osteopontin, osteocalcin mRNA and Type II Collagen were detected in chondrocyte-like cells. Whileosteocalcin mRNA was absent in chondrocytes in the enodchondral ossification of the fetal rat tibia. These results suggest that the chondrocyte-like cells in the BMP induced cartilage like tissue demonstrate the two phase funciton of both chondrocytes and osteoblast. The inductive cartilage-like tissue may be deisgnated as chondro-osseous cells in stead of chondro-cytes. The heterotopic chondro-osseous tissue formation induced by rhBMP-2 is process that differs from that of normal endchondral ossification in respect to cell differentiation and function. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (40 results)

All Other

All Publications (40 results)

  • [Publications] Nagatsuka,H.: "Immunohistochemical study of cytokeratin patterns in follicular and plexiform ameloblastoma." J.Hard Tissue Biol.4. 50-56 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 今村高行: "骨誘導蛋白(rhBMP-2)による異所性骨形成と骨基質蛋白遺伝子の発現-In situハイブリダイゼーション法による検討-" 岡山歯学会雑誌. 14. 1-13 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagai,N.: "Gene expression of bone matrix protein mRNA during BMP induced chondrogenesis and osteogenesis by in situ hybridization." J.Hard Tissue Biol.4. 15-23 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 永井教之: "アパタイト-骨結合界面の初期石灰化とBMPによる異所性骨誘導" J.Hard Tissue Biol.4. 79-90 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hong-In Shin: "The effect of enzymatic treatment on immunohistochemical staining of collagens in mandibular condyle of rats." J.Hard Tissue Biol.4. 15-23 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue M.: "Histopathologic and immunohistochemical study of heterotopic chondro-osseous tissue formation induced by S-200 BMP" J.Hard Tissue Biol.5. 1-6 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagai N.: "Expression of osteopontin and osteocalcin mRNA in heterotopic hard tissue formation induced by rhBMP-2 and purified bovine BMP" Journal of oral pathology and medicine. 25. 273 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nagai N.: "Gene expression of amelogenin in odontogenic tumors" Journal of oral pathology and medicine. 25. 273 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 永井教之: "分子量早期限定部分精製BMPの性状と骨I型コラーゲンキャリア" J.Hard Tissue Biol.5. 163-173 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 永井教之: "口腔扁平上皮癌と口腔粘膜上皮異形成におけるp53およびRb癌抑制遺伝子産物の免疫組織化学的検討" 岡山歯学会雑誌. 15. 1-8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 石割裕三: "幼弱マウス下顎頭軟骨におけるI型,II型,X型コラーゲン遺伝子の発現-In situ hybridization方による検討-" 岡山歯学会雑誌. 15. 53-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Hayashi: "Oncogene and tumor suppressor gene products in ameloblastoma : Correlation with cell kinetics" J.Okayama Dent.Soc.Vol.14 (1). 15-26 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nagai: "Gene Expression of Bone Matrix Protein mRNA during BMP Induced Chondrogenesis and Osteogenesis by in situ Hybridization" J.Hard Tissue Biol.Vol.4 (1). 15-23 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hong-In Shin: "The Effect of Enzymatic Treatment on Immunohistochemical Stainning of Collagens in Mandibular Condyle of Rats" J.Hard Tissue Biol.Vol.4 (1). 8-14 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Yonemoto: "Immunohistochemical study of p53 tumor suppressor gene product and proliferating cell nuclear antigen in oral squamous cell carcinoma" Jpn.J.Oral Maxillofac.Surg.Vol.41 (9). 46-50 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.SAKAE: "Crystallographic Properties and Cell Affinity Study of Synthetic alpha-tricalcium Phosphate, Tetracalcium Phosphate and Fluorapatite" J.Hard Tissue Biol.vol3 (2). 73-78 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Inoue: "Histopathological study of cemento-ossifying fibroma and ossifying fibroma in jaw bone" J.Hard Tissue Biol.Vol.4 (3). 9-14 (1885)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nagai: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP and early calcificaiotn on surface of apatite and bone" J.Hard Tissue Biol.Vol.4 (3). 79-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Hayashi: "Immunohistochemical expressions of PCNA in ameloblastoma and malignant ameloblastoma" J.Jpn.Stomatrol.Soc.Vol.44 (1). 7-13 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.KUBOKI: "Two Distinctive BMP-carriers Induce Zonal Chondorogenesis and Membranous Ossificaotn, Respectively ; Genometorical Factors of Materiales for Cell Differention" Connective Tissue Research. vol.31 (3). 1-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.MURATA: "Histopathological Studies of Heterotopic Bone Formation Induced by BMP Carrier Composites" J.Hard Tissue Biol.vol3 (2). 67-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Takagi: "Effects of Bovine Amelogrnin and Enamelin on in vivo Mineralization in Gel" J.Hard Tissue Biol.Vol.5 (1). 15-22 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Inoue: "Histopathological and immunohistological study of heterotopic chondro-osseous tissue formation induced by S-200 BMP" J.Hard Tissue Biol.Vol.5 (1). 1-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nagai: "Ultrasrtuctural study of inductive bone interface between bone morphogenic protein binding hydroxyapatite and heterotopic tissue" J.Hard Tissue Biol.Vol.5 (2). 102-109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nagai: "Characterization of bovine extracted BMP and development of bone collagen I carrier" J.Hard Tissue Biol.Vol.5 (2). 163-173 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Nagai: "Immunohistochemical study of p53 and Rb tumor suppressor gene products in oral squamous cell carcinoma and epithelial dysplasia" J.Okayama Dent.Soc.Vol.15 (1). 1-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Ishiwari: "Localized expression of type I,II and X collagen genes in mandibular condyle of immature mice" J.Okayama Dent.Soc.Vol.15 (1). 53-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Komuro: "Experimental study of bone formations by BMP-HAP composite in dog mandibular bone cavity" J.Jpn.Soc.Oral Implant.Vol.9 (2). 321-327 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Nosaka: "Histological and morphometrical study of bone formation in rat subcutaneous tissue by BMP-collagen particles" J.Hard Tissue Biol.Vol.5 (3). 188-194 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue M.: "Histopathologic and immunohistochemical study of heterotopic chondro-osseous tissue formation induced by S-200 BMP" J.Hard Tissue Biol.5. 1-6 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagai N.: "Expression of osteopontin and osteocalcin mRNA in heterotopic hard tissue formation induced by rhBMP-2 and purified bovine BMP" Journal of oral pathology and medicine. 25. 273- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagai N.: "Gene expression of amelogenin in odotogenic tumors" Journal of oral pathology and medicine. 25. 273- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 永井教之: "分子量早期限定部分精製BMPの性状と骨I型コラーゲンキャリア" J.Hard Tissue Biol.5. 163-173 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 永井教之: "口腔扁平上皮癌と口腔粘膜上皮異形成におけるp53およびRb癌抑制遺伝子産物の免疫組織化学的検討" 岡山歯学会雑誌. 15. 1-8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石割裕三: "幼弱マウス下顎頭軟骨におけるI型,II型,X型コラーゲン遺伝子の発現 -In situ hybridization方による検討-" 岡山歯学会雑誌. 15. 53-62 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nagatsuka,H.: "Immunohistochemical study of cytokeratin patterns in follicular and plexiform ameloblastoma." J.Hard Tissue Biol.4. 50-56 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 今村高行: "骨誘導蛋白(rhBMP-2)による異所性骨形成と骨基質蛋白遺伝子の発現-In situハイブリダイゼーション法による検討-" 岡山歯学会雑誌. 14. 1-13 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagai,N.: "Gene expression of bone matrix protein mRNA during BMP induced chondrogenesis and osteogenesis by in situ hybridization." J.Hard Tissue Biol.4. 15-23 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 永井教之: "アパタイト-骨結合界面の初期石灰化とBMPによる異所性骨誘導" J.Hard Tissue Biol.4. 79-90 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hong-In Shin: "The effect of enzymatic treatment on immunohistochemical staining of collagens in mandibular condyle of rats." J.Hard Tissue Biol.4. 15-23 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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