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Study on the cooperative bactericidal activities between neutrophis and antibiotics, electrolyzed-oxidizing water in serious infection disease

Research Project

Project/Area Number 07457482
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHIROSAKI UNIVERSITY

Principal Investigator

KIMURA Hiroto  Hirosaki University School of Medicine, Professor, 医学部, 教授 (90142851)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordsinfectious disese / MRSA / neutrophil / antibiotic / electrolyzed-oxidizing water / cooperative bactericidal activity / active oxygen production / flow cytometry
Research Abstract

O_2^<・->production abnormalities of neutrophils in peripheral blood with severely infectious disease in maxillofacial region were investigated by CLA dependent chemiluminescence method. The effect of antibiotics on O_2^<・->production of neutrophils was also studied. Furthermore, bactericidal effects of electrolyzed-oxidizing water on Stapylococcus aureus (S.aureus) were investigated by O_2^<・->production of neutrophils and expression of integrin molecules.Results were as follows.
1. O_2^<・->production of neutrophils obtained from 12 patients with oral cancer and 6 patients with mandublar osteomyelitis showed lower values than heal thy volunteers. Six of 12 patients with oral cancer were suffered from postoperative infection by MRSA.
2. Four antibiotics (ASPC,NTL,ISP,FLRX) increased O_2^<・->production of neutrophils to show cooperative bactericidal activities. And three of them (ASPC,NTL,FLRX) increased the expression of C3b recepter on the suface of neutrophil membrane.13EA04 : 3. O_2^<・->production of neutrophils accompanying with phagocytesis of S.aureaus (1*10^8/ml) , which were pretreated by electrolyzed-oxidizing water, showed 50% of bacteria was injured by this treatment. The expression of integrin molecules' (CD11b and CD18) on the surface of neutrophil membrane with phagocytosing S.aureus revealed that 70-80% of bacteria lost their biological activities by the treatment with electrolyzed-oxidizing water.
4. Surgical wound infected by MRSA was remarkably improved by washing with electrolyzed-oxidizing water. In treating MRSA infection patients, optimal dosage of Vancomycin^<<encircledR>>was determined by monitoring its concentration in peripheral blood.
These results suggested that it was useful to choose antibiotics based on the cooperative activity which increased O_2^<・->production of neutrophils. Electrolyzed-oxidizing water showed fatal effects on peccant bacteria, therefore, its clinical use was more recommended.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 五十嵐恵一: "掌蹠膿疱症を合併した慢性下顎骨骨髄炎の1例-臨床経過ならびに好中球機能の検討-" 日本口腔科学会雑誌. 44. 429-433 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中野靖子: "顎口腔領域におけるMRSA感染症に対する塩酸バンコマイシンの使用経験" 日本歯科薬物療法学会雑誌. 16(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Keiichi IGARASHI: "A case of chronic osteomyelitis of the mandible associated with pustulosis palmaris et plantaris -Report of clinical course and study on neutrophil function-" J.Jpn.Stomatol.Soc.44-3. 429-433 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuko NAKANO: "Clinical experience of Vancomycin in MRSA infection in oral and maxillofacial region" Oral Therapeutics and Pharamacology. 16-1 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary

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Published: 1995-04-01   Modified: 2016-04-21  

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