• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Osteonectin Degrading Activity in Calf Bone Matrix

Research Project

Project/Area Number 07457498
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionTsurumi University

Principal Investigator

ISHIBASHI Katsunori  School of Dental Medicine, Tsurumi University Professor, 歯学部, 教授 (20013980)

Co-Investigator(Kenkyū-buntansha) YAMACHIKA Shigeo  School of Dental Medicine, Tsurumi University Assistant, 歯学部, 助手 (60182565)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥5,700,000 (Direct Cost: ¥5,700,000)
Keywordscalf bone matrix / non-collagenous protein / osteonectin / proteinase
Research Abstract

Soluble proteins were sequentially extracted from demineralized calf bone matrix, with Tris buffer, NaCl solution and guanidine HCl solution. These extracts were concentrated for enzymography, but the guanidine HCl extract was muddied by this manipulation. Then the extract was separated GS (supemate) and GR (residue)-extract by centrifuge. Proteolytic activities in these extracts were investigated by means of enzymograply using gelatin or casein as a substrate. Identical proteolytic active bands were detected on the enzymograms of each the NaCl extract (N-extract), GS-extract and GR-Extract. However, when the purified osteonectin was used as substrate, a proteolytic activity was detected only in the GR-extract. These results would indicate that the presence of a specific osteonectin-lytic proteinase in bone. The enzyme needed Ca ions for activity and was inhibited by EDTA and 1,10-phenanthoroline, it would be an active type metalloproteinase which was bound to bone collagenous matrix Osteonectin degraded fragments were observed 26k, 22k, 20k, 16k, and 14k dalton bands by SDS-PAGE.Sinoe some of these bands were doserved in GR-extract, degraded fragments were bound more tighty than intact osteonectin. This result suggested that osteonectin might be degraded for increase ability of binding to collagen.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 山近重生: "ウシ骨基質中のオステオネクチン分解酵素活性に関する研究" 鶴見歯学. 22. 235-251 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shigeo YAMACHIKA: "Osteonectin Degrading Activity in Calf Bone Matrix" Tsurumi Univ.Dent.J.22 (2). 235-251 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山近重生: "ウシ骨基質中のオステオネクチン分解酵素活性に関する研究" 鶴見歯学. 22巻2号. 235-251 (1996)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi