| Project/Area Number |
07457500
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | OSAKA DENTAL UNIVERSITY |
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Principal Investigator |
OKANO Hakuro Osaka Dental University, Dentistry, Professor, 歯学部, 教授 (40066981)
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| Co-Investigator(Kenkyū-buntansha) |
ARIKA Takumi Osaka Dental University, Dentistry, Assistant, 歯学部, 助手 (70184281)
NAKAJIMA Masahiro Osaka Dental University, Dentistry, Assistant, 歯学部, 助手 (10188964)
MORITA Shosuke Osaka Dental University, Dentistry, Assistant Professor, 歯学部, 講師 (90148461)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1995: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | odontogenic tumor / molecular biology / oncogene / growth factor / cell culture |
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| Research Abstract |
Under the diagnosis of Ameloblastoma, we have succeeded the initial-culture in 7 cases out of 9. These cultures showed typical colonies separated from surrounding fibroblasts. Tumor cells usually proliferated for a certain period and be taken over by fibroblasts. We have freezed-stored these tumor cells and recovered on several cell adhesion molecule coated plates. Ameloblastoma grew well on collagen coated plates, while they shortly disappeared on fibronectin coated and non coated dishes. These results indicated that Ameloblastoma could be cultured on plastic dishes and be supplied for biochemical and molecular analysis in a certain condition. We have further investigated better environmental condition using the co-culture system with bone because of its natural growth circumstances. Immunohistochemical analysis demonstrated that cytoplasm of tumor cells and fibroblasts were positive for EGF receptor, however, no signal of EGF,TGF-beta, p53 and bcl-2 was found on Ameloblastoma. For molecular analysis, RT-PCR were performed with Osteopontin, Osteonectin, Human Papilloma Virus Type 16 (HPV16) and Amelogenin probes. Out of 7 cases, 3 showed positive for Osteopontin, 2 for Osteonectin, 1 for HPV 16 and non for Amelogenin. On the bases of these results, we have performed in situ hybridization and only 1 showed visible results. We are now searching for best conditions for demonstrating better in situ histology.
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