| Project/Area Number |
07457501
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
UEDA Yutaka Osaka Dental University, Faculty of Dentistry, Professor, 歯学部, 教授 (10067001)
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| Co-Investigator(Kenkyū-buntansha) |
MOMOTA Yoshihiro Osaka Dental University, Faculty of Dentistry, Assistant, 歯学部, 助手 (60247880)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Nociception / Cloning / Differential display / STAT family / Cytokine signaling / JAK family / Trigeminal ganglion / Cytokine receptor / Pain / Inflammation / Primary Sensory Neuron / Trigeminal spinal nucleus Caudalis / pain / chronic constristion injury / cloning / Differential display / Rat |
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| Research Abstract |
This research aimed at analizing of pain sensory system. We examined cloning of genes involved in nociception by differential display method. We got a lot of distinctive bands. But we have not got distinctive genes yet. The localization of some cytokine receptors and their downstream intracellular signaling molecules was examined in the rat trigeminal ganglion. Amongcytokine receptor components, we examined signal transduction subchain, gp130, IL-2Rgamma and IL-5Rbeta, which are common to respective groups of cytokine receptors. Most of the sensory ganglion neurons expressed gp130, but not IL-2Rgamma nor IL-5beta. We further examined the localization of Janus kinase family members which were reported to be associated with various kind of cytokine receptors and are thought to be implicated in majorcytokine receptor-signaling pathways. While JAK1 and Tyk 2 were expressed in all type of neurons, JAK2 was predominantly expressed in the small neurons. In addition, JAK3 immunoreactivity was only found in satellite cells. The present results indicate that most of neurons express gp130, and that the localization of JAK family members differs with the cell type. This also suggests that the cytokine receptor-signaling pathway may be different in neuronal and glial cells.
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