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Molecular biological and histomorphometrical study on bone remodeling and root resorption during orthodontic tooth movement.

Research Project

Project/Area Number 07457508
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionThe UNIVERSITY of TOKUSHIMA

Principal Investigator

TAKANO Teruko  The UNIVERSITY of TOKUSHIMA SCHOOL OF DENTISTRY,PROFESSOR, 歯学部, 教授 (00127250)

Co-Investigator(Kenkyū-buntansha) TENSHIN Satoru  The UNIVERSITY of TOKUSHIMA SCHOOL OF DENTISTRY,ASSOCIATE PROFESSOR, 歯学部, 助教授 (40197819)
相馬 俊一  大阪大学, 歯学部・附属病院, 助手 (90236141)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1995: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsOSTEOPONTIN / OSTEOCALCIN / OSTEONECTIN / IN SITU HYBRIDIZATION / MORPHOMETRY / EXPERIMENTAL TOOTH MOVEMENT / osteonectin / osteocalcin / osteopontin / 実験的歯の移動 / calcium sensing receptor / 骨基質蛋白遺伝子 / histomorphometry
Research Abstract

In the present study, the gene expression for osteopontin (OPN) and osteocalcin (OSC) in the alveolar bone was investigated by in situ hybridization histochemistry with non-radioisotopic digoxigenin 11-UTP labeled cRNA prove for OPN and OSC in rat maxillary dent-alveolar unit containing molars and intact PDL following a single loading to produce a well synchronized wave of complete remodeling. From these experiments we found osteocytes respond to mechanical loading in vivo with expression of mRNA for OPN but not for OSC,to associate with subsequent bone resorption. On day 0 an intense positive signal of mRNA for OSC but not OPN was demonstrable at osteoblasts on the distal surface of the interradicular septum of buccal roots of the maxillary first molar of 7-week-old rat. After insertion of an elastic band between the first and second molars the PDL facing the distal surface of the interradicular septum was compressed and the periodontal space on day 1 became narrower than that on day … More 0. At the time the positive signal of OSC mRNA disappeared from cells covering the distal surface of interradicular septum under the compressive strain environment. On the contray, a positive signal of mRNA for OPN was intensely expressed at oseocytes within the matrix in the distal half of the interradicular septum but not in the mesial half, and around the apex of buccomesial root, where the change of consequent compressive strains following a tipping movement of the first molar must occur. Thses osteocytes had large and roudish nuclei, and their osteocyte lacunae became enlarged compared with those on day 0. On day 3, as the compression of PDL continued, lining cells disappeared from the bone surface facing PDL and bone resorption on the distal region of the interradicular septum actively occured. The undermining resorption areas of alveolar bone were prominently eveident, and there were many osteoclasts making Howship's lacunae and a plenty of mononuclear cells around resorbing bone surfaces, where bone matrix containing OPN mRNA expressing osteocytes had been present on day 1. A positive signal of OPN mRNA expressed intensely in osteocytes, multinucleated cells adjacent to Howship's lacunae, designated as osteoclasts, and mononuclear cells on day 3. On the contrary, gene expression for OSC could not be observalbe at these areas.
These results suggest that at the beginning of a remodeling cycle stimulated by mechanical force, the OPN in osteocytes promotes osteoclast attachment and activation (stage 1), leading to the resorption phase (stage 2) and that osteocytes play an important role for mechanosensor. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Ying Ding,Shunichi Soma,Teruko Takano-Yamamoto,et al: "Effects of Salvia Miltiorrhiza Bunge(SMB)on MC3T3-E1 cells." J.Osaka Univ.Dent Sch.35(1). 21-27 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山本照子: "歯の移動と歯周組織のリモデリング" 四国歯学会雑誌. 151-162 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Ohba,Tomoko Ohba,et al: "Inhibitory mechanisms of H^+-ATPase inhibitor bafilomycin A1 and carbonic anhydrase II inhibitor acetazolamide on experimental bone resorption" FEBS Lett.387. 175-178 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.MURATA,et al: "Fluoridation of Hydroxyapatite Powder by Ammonium Hexafluorosilicate" Caries Research. 30. 465-470 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunichi Soma,Susumu Matsumoto,Teruko Takano-Yamamoto: "Conditioned medium of streched calvarial bone cells enhances osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures" Arch.Oral.Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takasi Yamashiro Teruko Takano-Yamamoto: "Differential response of mandibular condyle and femur to estrogen deficiency in young rats" Journal of Dental Research. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 分担執筆 山本照子: "エストロゲン 骨形成と骨吸収及びそれらの調節因子,2" 広川書店, 16 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ying Ding, Shunichi Soma, Teruko Takano-Yamamoto, Susumu Matsumoto, Mamoru Sakuda: ""Effects of Salvia Miltiorrhiza Bunge (SMB) on MC3T3-E1 cells"" J.Osaka Univ.Dent.Schl.35. 21-27 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teruko Takano-Yamamoto: ""Tooth movement and periodontal tissue remodeling"" Shikoku Society of Dental Research. 8-2. 151-162 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuo Ohba, Tomoko Ohba, Koji Sumitani, Kahori Tagami-Kondoh, Kenji Hiura, Yoshiki Miki, Hisao Kakegawa, Teruko Takano-Yamamoto, Nobuhiko Katunuma: ""Inhibitory mechanisms of H^+-ATPase inhibitor bafilomycin A1 and carbonic anhydrase II inhibitor acetazolamide on experimental bone resorption"" FEBS Letters. 387. 175-178 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroko Murata, Kunio Ishikawa, Satoru Tenshin, Shinya Horiuchi, Masaichi Nakanishi, Kenzou Asaoka, Terushige Kawata, Teruko Takano-Yamamoto: ""Fluoridation of Hydroxyapatite Powder by Ammonium Hexafluorosilicate"" Caries Research. 30. 465-470 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shunichi Soma, Susumu Matsumoto, Teruko Takano-Yamamoto: ""Conditioned medium of stretched calvarial bone cells enhances osteoclast-like cell formation induced by parathyroid hormone in mouse bone marrow cultures."" Arch.Oral.Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takasi Yamashiro and Teruko Takano-Yamamoto: ""Differential response of mandibular condyle and femur to estrogen deficiency in young rats."" Journal of Dental Research. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Teruko Takano-Yamamoto: Estrogen "BONE SCIENCE" -bone formation and resorption and their regulatory factor-Vol.2.Hirokawa Publishing Co., 347-362 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ying Ding,Shunichi Soma,Teruko Takano Yamamoto,et al.: "Effects of Salvia Miltiorrhiza Bunge (SMB) on MC3T3-E1 cells." J.Osaka Univ.Dent Sch.35(1). 21-27 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山本照子: "歯の移動と歯周組織のリモデリング" 四国歯学会雑誌. 151-162 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuo Ohba,Tomoko Ohba,et al.: "Inhibitory mechanisms of H^+-ATPase inhibitor bafilomycin Al and cabonic anhydrase II inhibitor acetazolamide on experimental bone resorption" FEBS Lett.387. 175-178 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.MURATA,et al.: "Fluoridation of Hydroxyapatite Powder by Ammonium Hexafluorosilicate" Caries Research. 30. 465-470 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shunichi Soma,Susumu Matsumoto,Teruko Takano-Yamamoto: "Conditioned medium of strestched calvarial bone cells enhances osteoclast-like cell formation induced by parathyuroid hormone in mouse bone marrow cultures" Arch.Oral.Biol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takasi Yamashiro,Teruko Takano-Yamamoto: "Differential response of mandibular condyle and femur to estrogen deficiency in young rats" Journal of Dental Research. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 分担執筆山本照子: "エストロゲン 骨形成と骨吸収及びそれらの調節因子,2" 広川書店, 16 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 山本照子: "歯の移動と歯周組織のリモデリング" 四国歯学会雑誌. 8. 151-162 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoshiki Miki, et al.: "DIFFERENTIATION-DEPENDENT RESPONSE TO EXTRACELLULAR CALCIUM IN CULTURED RAT OSTEOCYTES" B.B.R.C.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] 柳浩成 et al.: "Effect of Retinoic Acid on Human Gingival Fibroblasts in collagen gel" Journal of Dental Research (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] H. MURATA, et al.: "Fluoridation of Hydroxyapatite Powder by Ammonium Hexafluorosilicate" Caries Research (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Kawakami, T. Takano-Yamamoto: "Local injection of 1.25-dihydroxy vitamin D3 enhanced bone formation on experimental tooth movement in rats" J. Dent Res. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Ding, S. Soma, T. Takano-Yamamoto et al.: "Effects of Salvia Miltiorrhiza Bunge (SMB) on MC3T3-E1 cells" J. Osaka Univ Dent Schl.35. 21-27 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 分担執筆 山本照子: "エストロゲン、骨形成と骨吸収及びそれらの調節因子 2巻" 広川書店, 16 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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