| Project/Area Number |
07457533
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KURIHARA Kenzo Hokkaido Univ., Fac.of Pharmaceut.Sci., 薬学部, 教授 (00016114)
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| Co-Investigator(Kenkyū-buntansha) |
SHOUJI Takayuki Hokkaido Univ., Fac.of Pharmaceut.Sci., 薬学部, 教務職員 (00241349)
KASHIWAYANAGI Makoto Hokkaido Univ., Fac.of Pharmaceut.Sci., 薬学部, 助手 (20169436)
MATSUOKA Ichiro Hokkaido Univ., Fac.of Pharmaceut.Sci., 薬学部, 助手 (40157269)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1995: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | phosphatidic acid / lipoproein / pheromone / urine / vomeronasal organ / cyclic AMP / IP3 / rat / ファスファチジン酸 / G蛋白質共役型受容体 / 味細胞 / 嗅細胞 / 情報変換機構 / グアニル酸シク-ラーゼ / クローニング / G-蛋白質共役型受容体 / カエル |
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| Research Abstract |
1) We examined the effects of lipoprotein composed of phosphatidic acid (PA) and beta-lactglobulin (LG) on bitterness of various chemicals by measuring nerve activities of frog glossopharyngeal nerve. PA-LG inhibited the bitterness of various drugs, but did not inhibit that of salts such as MgCl_2, CsCl and tetraethylammonium, suggesting that the receptor for bitter salts is different from other bitter compounds.
2) We also examined whether a single olfactory cell responds only to specific odorants. The results obtained showed that a single olfactory cell respond to many different species of odorants, suggesting that a single olfactory cell has many different receptors.
3) We prepared slice of rat vomeronasal epithelium and measured currents in response to cAMP and IP3. Injection of cAMP did not elicit current, but that of IP3 induced inward current, Female rat vomeronasal receptor cell responded to urine from male rat. This response was inhibited by inhibitors for production of IP3. These results suggest that IP3 may be a second messenger in reception of urinary pheromone in female rat vomeronasal receptor cell.
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