| Project/Area Number |
07457561
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
HOSAKA Seijin Tokyo Medical & Dental University, Professor, 医学部, 教授 (80013944)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Kenji Tokyo Medical & Dental University, assistant, 医学部, 助手 (20242183)
SHIBA Kiyoko Tokyo Medical & Dental University, assistant professor, 医学部, 助教授 (00013988)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | Capillary Electrophoresis / Urinary protein / 薬剤性腎症 / 尿タンパク / 薬剤性腎障害 / キヤピラリー電気泳動 / 早期発見 |
|---|
| Research Abstract |
This research project was carried out to detect renal impairment in early stage caused by drug administratin using urinary proteins profiles obtained by capillary electrophoresis.
The first year, we examined the basic conditions for quantitative analysis of urinary protein fractions by capillary electrophoresis and established the optimal conditions. We also established a new staining method sensitive enough to detect urine proteins bands of normal subjects without concentration. This method contributed markedly to this project in the following two years. During the second year, we found 44 peaks in normal urine by capillary electrophoresis and we identified them successively. In this year, we found that protein fractions analyzed by cellulose acetate membrane were useful for early detection of diabetic nephropathy and its prognosis. During the 3rd year, we analyzed the urine samples obtained from patients who were treated by administration of the drugs which often caused renal complications. The research study is not finished at this stage, but the results will be obtained within 1998.
From the results we obtained at present time, we believed that the capillary electrophoresis is highly useful not only for early detection of drug induced renal impairment but also for typing and prognosis of nepheropathy.
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