| Project/Area Number |
07457563
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Gifu University |
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Principal Investigator |
NOMA Akio Gifu University School of Medicine, Professor, 医学部, 教授 (30208384)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Kuniaki Gifu University School of Medicine, Assistant, 医学部, 助手 (80262765)
SHIMOKAWA Kuniyasu Gifu University School of Medicine, Assistant Professor, 医学部, 助教授 (70021376)
牧野 和彦 岐阜大学, 医学部, 助手 (80181618)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥5,800,000 (Direct Cost: ¥5,800,000)
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| Keywords | Lipoprotein (a), Lp (a) / Acute phase reactant / Immunohistochemistry / Tissue repair / Endothelial cell / Migration / Proliferation / Angiogenesis / 遊走及び増殖 / アポ(a)イソ型 |
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| Research Abstract |
Although we have reported transient increases of plasma lipoprotein (a) [Lp (a)] after acute myocardial infarction and surgical operations, the significance of these increases remains unclear. The purpose of the present study was to clarify the physiological roles of Lp (a) in tissue repair.
Many samples from inflamed tissues were examined by immunohistochemical techniques. The immunohistochemical features of granulation tissues were characterized by different stages of wound healing. In the first stage, immunoreactivities for anti-apo (a) and-apo B were weak and focal, whereas those for anti-plasminogen and-fibrinogen were strong and widespead on the tissue surface. In the second stage, granulation tissue were covered with loose fibrous connective tissue, and markedly positive staining for Lp (a) was observed closer to the surface, suggesting that Lp (a) may prevent external fibrinolysis. Lp (a) was also found in endothelial cells and the extracellular space of small vessels underlying the fibrous connective. tissues. In the last stage of healing, apo (a) and apo B were not detectable in completely organized tissues. These findings suggest that Lp (a) plays a role in the wound healing.
In in vitro study with human umbilical vein endothelial cell (HUVEC), the stimulatory effects of Lp (a) on the migration and proliferation of endothelial cells were observed. These effects were not affected with its isoforms. Anti-bFGF,added in the incubation medium treated with Lp (a), suppressed the Lp (a)-induced HUVEC migrations and proliferations, but the inhibitions were not complete. Pertussis toxin, that blocks the activity of bFGF,also suppressed Lp (a)-induced HUVEC migration. These findings suggest that Lp (a) stimulates the bFGF-mediated HUVEC migration and proliferation, and may promote the angiogenesis.
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