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Molecular mechanism of persistent infection of SSPE virus

Research Project

Project/Area Number 07457581
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionNiigata University

Principal Investigator

ABE Satoshi  Niigata University, Brain Res Inst, Research Associate, 脳研究所, 助手 (90202663)

Co-Investigator(Kenkyū-buntansha) KUMANISHI Toshiro  Niigata University, Brain Res Inst, Professor, 脳研究所, 教授 (40018601)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1996: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsSubacute screlosing panencephalitis / SSPE / Persistent infection / Gene mutation
Research Abstract

Subacute sclerosing panencephalitis (SSPE) is a chronic progressive disease of the central nervous system (CNS) caused by a persistent infrction of measles virus or a variant of measles virus. In this study, RT-PCR (Reverse transcription-coupled polymerase chain reaction) and cDNA cloning were used to examine the matrix (M) protein gene of measles virus in the brain and lymph node from a case of SSPE.In the examination of the brain, ten independent clones of the entire length of the coding region of the M protein gene were sequenced. The identical multiple mutations were revealed in 57 of 1008 nucleotides in comparison with Edmonston strain of measles virus. Ninety-three percent of these mutations was transition (U to C and A to G), so-called biased hypermutation. The nucleotide differences resulted in amino acid changes with considerable frequency. The M protein of all clones was found to terminate prematurely due to a nonsense mutation at codon 114 although the initiation codon was preserved. In the lymph node, twelve clones had identical mutations with brain clones and had 2 to 19 additional mutations. In 8 out of 12 clones, the termination point of the M protein was found to change due to the other nonsense mutation at codon 296. Another five major structural protein genes (NP,P,F,HA and L) were also detected in both brain and lymph node in our case. These findings suggest that in addition to the central nervous system, the lymph node may also have a role in the progression of SSPE.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Abe S: "SSPE : Detection of measles virus gene in brain and lymph node by RT-PCR" Brain Pathology. 7 (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 阿部 聰: "SSPE :中枢神経経およびリンパ節におけるウイルス遺伝子の検出" Neuropathology. 17 (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsumanuma I: "Infrequent mutation of Waf1/p21 gene, a CDK inhibitor gene, in brain tumors" Neurol Med Chir. 37. 150-157 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanuma N: "Pretreatment with T cell receptor peptides using a conventional immunization protocol dose not induce effective protection against autoimmune encephalitis" Cellular Immunology. 168. 85-90 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsumoto Y: "Characterization of CD4^- CD8^- T cell receptor αβ^+ T cells appearing in the subarachnoid space of rats with autoimmune encephalomyelitis" Eur J Immunology. 26. 1328-1334 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikeda K: "Comparison of the three mouse G-protein-activated K^+ (GIRK) channels and functional couplings of the opioid receptors with the GIRK1 channel" Ann NY Acad Sci. 801. 95-109 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Abe, S.: "SSPE : Detection of measles virus gene in brain and lymph node by RT-PCR." Brain Pathology. 7(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Abe, S.: "SSPE : Detection of viral genome in the central nervous system and lymph node. (in Japanese)" Neuropathology. 17(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsumanuma, I.: "Infrequent mutation of Waf1/p21 gene, a CDK inhibitor gene, in brain tumors." Neurol Med Chir. 37. 150-157 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanuma, N.: "Pretreatment with T cell receptor peptides using a conventional immunization protocol does not induce effective protection against autoimmune encephalitis." Cellular Immunology. 168. 85-90 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsumoto, Y.: "Characterization of CD4^- CD8^- T cell receptor alphabeta^+ cells appearing in the subarachnoid space of rats with autoimmune encephalomyelitis." Eur J Immunology. 26. 1328-1334 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ikeda, K.: "Comparison of the three mouse G-protein-activated K^+ (GIRK) channels and functional couplings of the opioid receptors with the GIRK1 channel" Ann NY Acad Sci. 801. 95-109 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Abe S: "SSPE : Detection of measles virus gene in brain and lymph node by RT-PCR" Brain Pathology. 7(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 阿部聰: "SSPE: 中枢神経系およびリンパ節におけるウイルス遺伝子の検出" Neuropathology. 17(in press). Tsumanuma I (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Infrequent mutation of Waf1/p21 gene,a CDK inhibitor gene,in brain tumors20GB03:Neurol Med Chir: 37. 1997. (150-157)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tanuma N: "Pretreatment with T cell receptor peptides using a conventional immunization protocol does not induce effective protection against autoimmune encephalitis" Cellular Immunology. 168. 85-90 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsumoto Y: "Characterization of CD4-CD8-T cell receptor αβ+T cells appearing in the subarachnoid space of rats with autoimmune encephalomyelitis" Eur J Immunology. 26. 1328-1334 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ikeda K: "Comparison of the three mouse G-protein-activated K^+(GIRK) channels and functional couplings of the opioid receptors with the GIRK1 channel" Ann NY Acad Sci. 801. 95-109 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 阿部 聰: "Intravascular malignant lymphomatosisにおける腫瘍細胞の解析" Clinical Neurology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 阿部 聰: "Intravascular malignant lymphomatosisにおける免疫グロブリン再構成遺伝子の解析" Neuropathology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 阿部 聰: "Intravascular malignant lymphomatosis:免疫グロブリン再構成遺伝子の解析" 新潟医学会雑誌. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanuma N: "Pretreatment with T cell receptor peptides using a conventional immunization protocol does not induce effective protection against autoimmune encephalitis" Cellular Immunology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 遠藤純男: "EBウイルス感染により得られた脳原発悪性リンパ腫由来細胞の特性" Neuropathology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宮下 光太郎: "Intravascular malignant lymphomatosisの末梢血による診断と治療効果判定の可能性" Clinical Neurology. (in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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