• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular analysis of abnormal fracture healing process

Research Project

Project/Area Number 07457585
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionOsaka University

Principal Investigator

NOMURA Shintaro  Osaka University Medical School Associate Professor, 医学部, 助教授 (80159087)

Co-Investigator(Kenkyū-buntansha) HIROTA Seiichi  Osaka University Medical School Assistant Professor, 医学部, 助手 (50218856)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsfracture lrealing / osteopontin / osteosarcoma / osteoblast / transcription factor / calcification / chondrocyte / scurvy / オステオカルシン / オステオネクチン / ビタミンC / 骨折 / 内軟骨性骨化 / 膜性骨化 / コラーゲン / 遺伝子発現 / 骨形成不全 / 仮骨 / 炎症 / 偽関節
Research Abstract

Impaired fracture healing process was investigated by molecular histological techniques such as in situ hybridization. ODS (Osteogenesis Disorder Shionogi) rat was used for Scurvy model. Membranous ossification process in fracture healing was strongly inhibited by the depletion of vitamin C.Particularly, no expression of osteopontin, extracellular matrix protein located in the bone matrix, was detected. Early stages of endochondral ossification was not strongly inhibited. The unmber of chondrocytes in vitamin C depleted rats was comparable to that of normal rat. However, only a few number of hypertrophic chondrocytes which express osteopontin nRNA was observed. The results suggest the possibility that due to the impaired expression of osteopontin, calcification process in the scurvy was strongly inhibited. Furthermore, transcriptional factors regulating the expression of osteopontin gene was identified in vivo and in vitro. CBFA1, PU.1, MITF were cooperatively interacted with 5'-flanking region of osteopontin gene. No expression of osteopontin gene was detected in the knockout mice of cbfa-1 gene. This knockout mice showed almost no calcification in the bone tissue. The result also suggested the involvement of osteopontin to the calcification process. The biological role of osteopontin for the bone remodeling was also investigated. Enhanced expression of osteopnotin gene was observed by mechanical stress of "press" in the osteocytes. The result indicated that the osteocytes acted as sensory cells respond to echanical stress by espressing osteopontin gene.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Hirota, S: "Expression of bone matrix protein messenger RNAs in human breast cancers" Lab.Invest. 72. 64-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohara, R: "Identification of cells expressing cot proto-oncogenemRNA" J.Cell Science. 108. 377-385 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hashimoto, M: "Expression of vascular endothelial growth factor and its receptor mRNA in angiosarcoma" Lab.Invest. 73. 859-863 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ushio, H: "Mechanism of eosinophilia in mice infected with larval Haemophysalis longicornis ticks" Immunology. 84. 469-475 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tokunaga, K: "Human osteosarcoma (OST) induces reactive bone formation in xenograft system" Bone. 19. 447-454 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasui, N: "Distraction osteogenesis in a rat model three different models of ossification" J.of Bone and Joint Surgery. 79. 824-830 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Hirota: "Expression of bone matrix protein messenger RNAs in human breast cancers : Possible involvement of osteopontin in development of calcifying foci." Lab.Invest.72. 64-69 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Hirota: "Possible role of osteopontin for deposition of calcium phosphate in human pilomatricomas." J.Invest.Dermatol.105. 138-142 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Ilic.: "Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice." Nature. 377. 539-544 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Nakane: "High incidence of UVB-or chemical carcinogen-induced skin tumorigenesis in mice lacking the xeroderma pigmentosum group A (XPA) gene." Nature. 377. 165-168 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Nakase: "Spliced expression of bone morhpogenetic protein-4 in the process of bone formation in embryogenesis and fracture repair." Wound Repair and Regeneration. 4. 82-86 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Tsukamoto: "150-kD Oxygen-regulated protein is expressed in human atherosclrotic plaques and allows mononuclear phagocytes to withstand cellular stress on exposure to hypoxia and modified low density lipoprotein." J.Clin.Invest.98. 1930-1941 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Komori: "Targeted disruption of CBFA1/PEBP2alphaA gene results in complete lack of bone formation due to the maturational arest of osteoblasts." Cell. 89. 755-764 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sugimoto: "Impared expression of non-collagenous bone matrix protein mRNAs during fracture healing in ascorbic-acid deficient rats." J.Bone and Mineral Research.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sato: "Expression of bone matrix proteins mRNA during distraction osteogenesis." J.Bone and Mineral Research.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Takebayashi: "Resessire phenotype disployed by dominart negative MITF mutant is a result of impared nuclear localization potential" Mol.Cell.Biol. 16. 1203-1211 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Kimura: "Expression of oxytocin receptor in human pregnant wyometrium" Eudocrinology. 137. 780-785 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Nakajima: "Identification of a cell types of odontoma-like clustere in microplthaluic (mi/mi) mice using in situ nybridization" Pathal.Int.46. 743-750 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Tokunaga: "Human ostlosarcoma (OST) induces mouse reactive bone formation in xenognaft system," Bone. 19. 447-454 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Nomura: "Cellular localtzation of thrsmbopoietiu mRNA in the liver by in situ hybridization" Exp HaemaTol. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Takemura: "Localization of bone morpluogenetic protein-4 messenger RNA in developing mouse cochea" Hearing Res.95. 26-32 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hirota,S: "Expression of bone matrix protein messenger RNA in lymor lrest cencers Possible invol vement of osteoportin in dovelepment of colcifying fore" Lab.Invest.72. 64-69 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Satake,M: "Exprossion of Runt domain coding PEBp2αgenes in cells during tleymic development." Mol.Cell.Biol.15. 1662-1670 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohara,R: "Identification of the cells expressing cot proto-oncogene mRNA" J.of Cell Sci.108. 97-103 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ito.A: "Enhanced expression of CD34 messenger RNA by developing endotlelial cells of mice" Lab.Iuvest. 72. 532-538 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hirota,T: "Characterization of c-kit-positive neuroris in the dorxilroot gaiglion of mouse" Dev.Brain Res.85. 201-211 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hirota,S.: "Dossible role of osteoportin for deposition of calcium plorplate in human pilomatricras" T.Inuest.Dermatol. 105. 138-142 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi