| Project/Area Number |
07457588
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Tokai University |
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Principal Investigator |
TAMAOKI Norikazu Tokai University School of Medicine Professor, 医学部, 教授 (50055860)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Hitoshi Tokai University School of Medicine Assistant Professor, 医学部, 講師 (20191273)
NAKAMURA Masato Tokai University School of Medicine Assistant Professor, 医学部, 講師 (00164335)
UEYAMA Yoshito Tokai University School of Medicine Associate Professor, 医学部, 助教授 (30072408)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | brain tumor / multidrug resistance / MDR1 gene / blood brain barrier / molecular pathology / reversal of MDR / transgenic mouse / expreimental therapy / MDR / P糖蛋白質 / 膜能動輸送 / 化学療法 |
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| Research Abstract |
Human glioma usually shows intrinsic multidrug resistance due to the blood brain barrier (BBB), in which membrane-associated P-glycoprotein (PGp) encoded by human multidrug resistance gene MDR1 plays certain roles. We established severe combined immune deficient (SCID) mice harboring murine endothelin driven human MDR1 gene. Then, we are studying drug sensitivity to vincristine (VCR), doxorubicin (DOX) and nimustine (ACNU) in subcutaneously xenotransplanted human glioma. We examined the levels of MDR1 gene expression in the xenografts by reverse transcriptase-polymerase chain reaction, and the localization of PGp by immunohistochemistry. The localization of PGp was limited to the stromal vessels in the xenografts by immunohistochemistry. The in vivo chemosensitivity test, now going, will provide us some insights for PGp expressed on the stromal vessels as a major contributing factor to multidrug resistance in human glioma.
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