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Study on Ca^<2+> sensitivity of reperfusion hypertrophied immature heart

Research Project

Project/Area Number 07457599
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

ITOI Toshiyuki  Kyoto Prefectural University of Medicine Lecturer, 医学部, 講師 (10264780)

Co-Investigator(Kenkyū-buntansha) ONOUCHI Zenshiro  Kyoto Prefectural University of Medicine Professor, 医学部, 教授 (20079875)
周藤 文明  京都府立医科大学, 医学部, 助手 (20271166)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsimmature heart / reperfusion injury / myocardial metabolism / isolated perfused heart / myocardial hypertrophy / 肥大心 / 再灌流 / カルシウムイオン
Research Abstract

We developed chlonic pressure overloaded or volume overloaded heart on immature rabbits as a in situ model of congenital heart desease. The isolated hypertrophied heats subjected to overload were perfused and studied the mechanism of reperfusion injury in immature herat. was stuied Firstly, we studied effects of extracellular calcium concentrations on functional recovery after global ischemia. Secondly, the relation between intracardiac pH and Ca^<2+> sensitivity of contractile proteins in reperfused immature hearts.
Results
1)Increase of Ca^<2+> from 1.75mM to 2.5mM at the initiation of reperfusion after 40 min of global ischemia improved mechanical function of isolated one-week old rabbit hearts.
2)Na^+/H^+ inhibiter (amirolide), reported to improve mechanical function of adult heart, showed no remarkable effect on immature reperfused hearts.
3)No significant effect has also seen in addition of Cl^-/HCO3^- inhibiter (SITS) on reperfused one-week old rabbit hearts.
4)Ca^<2+> sensitizer (pimobendan) improved dramatically contractile function of reperfused immature rabbit herats.
5)We modified perfusion system to allow that high energy phosphates signal can be detected at each 4 min in ^<32>P-NMR mounted 25mm coil.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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